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showing 10 items of 10515 documents
Dual Antimicrobial and Antiproliferative Activity of TcPaSK Peptide Derived from a Tribolium castaneum Insect Defensin
2021
Antimicrobial peptides (AMPs) found in the innate immune system of a wide range of organisms might prove useful to fight infections, due to the reported slower development of resistance to AMPs. Increasing the cationicity and keeping moderate hydrophobicity of the AMPs have been described to improve antimicrobial activity. We previously found a peptide derived from the Tribolium castaneum insect defensin 3, exhibiting antrimicrobial activity against several human pathogens. Here, we analyzed the effect against Staphyloccocus aureus of an extended peptide (TcPaSK) containing two additional amino acids, lysine and asparagine, flanking the former peptide fragment in the original insect defensi…
S. Typhimurium virulence changes caused by exposure to different non-thermal preservation treatments using C. elegans
2017
The aims of this research study were: (i) to postulate Caenorhabditis elegans (C. elegans) as a useful organism to describe infection by Salmonella enterica serovar Typhimurium (S. Typhimurium), and (ii) to evaluate changes in virulence of S. Typhimurium when subjected repetitively to different antimicrobial treatments. Specifically, cauliflower by-product infusion, High Hydrostatic Pressure (HHP), and Pulsed Electric Fields (PEF). This study was carried out by feeding C. elegans with different microbial populations: E. coli OP50 (optimal conditions), untreated S. Typhimurium, S. Typhimurium treated once and three times with cauliflower by-product infusion, S. Typhimurium treated once and f…
Recovery Estimation of Dried Foodborne Pathogens Is Directly Related to Rehydration Kinetics.
2016
International audience; Drying is a common process which is used to preserve food products and technological microorganisms, but which is deleterious for the cells. The aim of this study is to differentiate the effects of drying alone from the effects of the successive and necessary rehydration. Rehydration of dried bacteria is a critical step already studied in starter culture but not for different kinetics and not for pathogens. In the present study, the influence of rehydration kinetics was investigated for three foodborne pathogens involved in neonatal diseases caused by the consumption of rehydrated milk powder: Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enteri…
SEA WATER TEMPERATURE RECORDS GATHERED DURING EXPERIMENTAL BOTTOM TRAWL SURVEYS IN THE STRAIT OF SICILY (MEDITERRANEAN SEA)
2009
Depth and sea water temperature values recorded by a Minilog system during several bottom trawl surveys carried out in the Strait of Sicily (Central Mediterranean Sea) in spring (MEDITS) and late summer (GRUND), were analyzed by using an ad hoc developed software (MiSeAT). Depth and temperature profiles were analyzed for each haul. Three sea water temperature (t°C) values were considered: the upper and lower water layers temperature, and the difference between them. Mean values by depth stratum and the corresponding standard deviations were estimated for the three parameters, then compared between the two seasons. Haul parameters recorded during the more heterogeneous GRUND surveys (due to …
Phylogeny and Mycotoxin Characterization of Alternaria Species Isolated from Wheat Grown in Tuscany, Italy
2018
Wheat, the main source of carbohydrates worldwide, can be attacked by a wide number of phytopathogenic fungi, included Alternaria species. Alternaria species commonly occur on wheat worldwide and produce several mycotoxins such as tenuazonic acid (TA), alternariol (AOH), alternariol-monomethyl ether (AME), and altenuene (ALT), provided of haemato-toxic, genotoxic, and mutagenic activities. The contamination by Alternaria species of wheat kernels, collected in Tuscany, Italy, from 2013 to 2016, was evaluated. Alternaria contamination was detected in 93 out of 100 field samples, with values ranging between 1 and 73% (mean of 18%). Selected strains were genetically characterized by multi-locus…
Proposal of an Architecture to Retrieve Information from Multiple Devices Using Matchmaking Algorithms
2007
Semantic Web services (SWS) propose to extend the traditional Web services technologies on the way to consolidate ontologies and semantics. This makes possible the selection, the integration and the dynamic invocation of services. In this way, services are able to dynamically adapt themselves to changes without human intervention. The main purpose of this paper is to present an algorithm for matching SWS. The algorithm uses the description of the services capabilities to match the semantic values. The traditional matching has been improving using ontologies witch constitutes a step further in the matching algorithms. To implement the algorithm an agent FIPA compliant architecture has been d…
HepatomiRNoma: The proposal of a new network of targets for diagnosis, prognosis and therapy in hepatocellular carcinoma
2015
Abstract: The diagnosis and treatment of hepatocellular carcinoma (HCC) underwent a huge advancement in the last years. Recently, microRNAs (miRNAs) have been also studied to provide a new tool for early diagnosis of high risk patients, for prognostic classification to identify those patients who benefit cancer treatment and for predictive definition to select the right targeted drug. In this review we revised all the available data obtained to explore the role of miRNAs in HCC. This analysis led to identification of miRNAs which could gain a diagnostic, prognostic or predictive role. The results of studies on miRNAs involved in HCC are initial and far from providing scientific evidences to…
Cholesterol Starvation and Hypoxia Activate the FVII Gene via the SREBP1-GILZ Pathway in Ovarian Cancer Cells to Produce Procoagulant Microvesicles
2019
AbstractInteraction between the transcription factors, hypoxia-inducible factor (HIF1α and HIF2α) and Sp1, mediates hypoxia-driven expression of FVII gene encoding coagulation factor VII (fVII) in ovarian clear cell carcinoma (CCC) cells. This mechanism is synergistically enhanced in response to serum starvation, a condition possibly associated with tumor hypoxia. This transcriptional response potentially results in venous thromboembolism, a common complication in cancer patients by producing procoagulant extracellular vesicles (EVs). However, which deficient serum factors are responsible for this characteristic transcriptional mechanism is unknown. Here, we report that cholesterol deficien…
A hybrid approach to semantic web services matchmaking
2008
AbstractDeploying the semantics embedded in web services is a mandatory step in the automation of discovery, invocation and composition activities. The semantic annotation is the “add-on” to cope with the actual interoperability limitations and to assure a valid support to the interpretation of services capabilities. Nevertheless many issues have to be reached to support semantics in the web services and to guarantee accurate functionality descriptions. Early efforts address automatic matchmaking tasks, in order to find eligible advertised services which appropriately meet the consumer’s demand. In the most of approaches, this activity is often entrusted to software agents, able to drive re…
Inhibitory Activity and Chemical Characterization ofDaucus carotasubsp.maximusEssential Oils
2017
The essential oils (EOs) of green seeds from Daucus carota subsp. maximus growing wild in Pantelleria Island (Sicily, Italy) were characterized. EOs were extracted by steam distillation, examined for their inhibitory properties against food-borne Gram-positive and Gram-negative bacteria and analyzed for the chemical composition by gas chromatography (GC) and mass spectrometry (MS). Undiluted EOs showed a large inhibition spectrum against Gram-positive strains and also vs. Acinetobacter spp. and Stenotrophomonas maltophilia. The minimum inhibition concentration (MIC) was in the range 1.25 â 2.50 μl/ml for the most sensitive strains. The chemical analysis indicated that D. carota subsp…