Search results for " Basic"

showing 10 items of 10515 documents

Polymorphonuclear leukocyte integrins in deep venous thrombosis

2005

The polymorphonuclear leukocytes (PMN) have a role in the pathophysiology of deep venous thrombosis (DVT). We examined the phenotypical expression of PMN beta2-integrins (CD l l a, CDl l b, CD 11c) in a group of 19 subjects with leg DVT. PMN cells were incubated with fluorescent monoclonal antibodies against CD11a, CD11b, CD11c, and the evaluation was made by flow cytofluorimetry. The same integrins were determined after in vitro activation with 4-phorbol 12-myristate 13-acetate (PMA) and N-formylmethionyl-leucyl-phenylalanine (fMLP). In DVT subjects, at baseline, the phenotypical expression of CD11b was decreased and that of CD11c increased when compared with normal controls. In normal su…

Male0301 basic medicineIntegrinsNeutrophilsmedicine.drug_classIntegrinCD11a030204 cardiovascular system & hematologyMonoclonal antibody03 medical and health scienceschemistry.chemical_compound0302 clinical medicinepolymorphonuclear leukocyte integrinmedicineHumansdeep venous thrombosiVenous ThrombosisbiologyCell adhesion moleculebusiness.industryhemic and immune systemsHematologyGeneral MedicineMiddle AgedN-Formylmethionine leucyl-phenylalaninemedicine.diseasePathophysiologyN-Formylmethionine Leucyl-PhenylalanineVenous thrombosis030104 developmental biologychemistryIntegrin alpha MImmunologybiology.proteinTetradecanoylphorbol AcetateFemalebusinesspolymorphonuclear leukocyte activation
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Canakinumab for the Treatment of Autoinflammatory Recurrent Fever Syndromes.

2018

BACKGROUND: Familial Mediterranean fever, mevalonate kinase deficiency (also known as the hyperimmunoglobulinemia D syndrome), and the tumor necrosis factor receptor-associated periodic syndrome (TRAPS) are monogenic autoinflammatory diseases characterized by recurrent fever flares. METHODS: We randomly assigned patients with genetically confirmed colchicine-resistant familial Mediterranean fever, mevalonate kinase deficiency, or TRAPS at the time of a flare to receive 150 mg of canakinumab subcutaneously or placebo every 4 weeks. Patients who did not have a resolution of their flare received an add-on injection of 150 mg of canakinumab. The primary outcome was complete response (resolution…

Male0301 basic medicineInterleukin-1betaFamilial Mediterranean fever0302 clinical medicineMonoclonalChildMedicine(all)Mevalonate kinase deficiencySubcutaneousMedicine (all)Interleukin-1betaAntibodies MonoclonalGeneral MedicineFamilial Mediterranean FeverRecurrent feverChild PreschoolFemaleTumor necrosis factor alphaDrugInflammatory diseases Radboud Institute for Molecular Life Sciences [Radboudumc 5]medicine.drugAdultAdolescentFeverInjections SubcutaneousHereditary Autoinflammatory DiseasesAntibodies Monoclonal HumanizedAdolescent; Adult; Antibodies Monoclonal/administration & dosage; Antibodies Monoclonal/adverse effects; Antibodies Monoclonal/therapeutic use; Child; Child Preschool; Dose-Response Relationship Drug; Double-Blind Method; Familial Mediterranean Fever/drug therapy; Female; Fever/drug therapy; Hereditary Autoinflammatory Diseases/drug therapy; Humans; Injections Subcutaneous; Interleukin-1beta/antagonists & inhibitors; Male; Mevalonate Kinase Deficiency/drug therapy; Young AdultAntibodiesInjectionsDose-Response RelationshipYoung Adult03 medical and health sciencesAll institutes and research themes of the Radboud University Medical CenterDouble-Blind MethodGeneral & Internal MedicinemedicineHumansPreschoolAdolescent; Adult; Antibodies Monoclonal; Child; Child Preschool; Dose-Response Relationship Drug; Double-Blind Method; Familial Mediterranean Fever; Female; Fever; Hereditary Autoinflammatory Diseases; Humans; Injections Subcutaneous; Interleukin-1beta; Male; Mevalonate Kinase Deficiency; Young Adult; Medicine (all)030203 arthritis & rheumatologyDose-Response Relationship Drugbusiness.industryHereditary Autoinflammatory DiseasesHyperimmunoglobulinemia Dmedicine.diseaseCanakinumab030104 developmental biologyImmunologyMevalonate Kinase Deficiencybusiness
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Perineuronal Nets Regulate the Inhibitory Perisomatic Input onto Parvalbumin Interneurons and γ Activity in the Prefrontal Cortex

2020

Parvalbumin-expressing (PV+) interneurons play a key role in the maturation and synchronization of cortical circuitry and alterations in these inhibitory neurons, especially in the medial prefrontal cortex (mPFC), have been found in different psychiatric disorders. The formation of perineuronal nets (PNNs) around many of these interneurons at the end of the critical periods reduces their plasticity and sets their connectivity. Consequently, the presence of PNNs must have an important impact on the synaptic input and the physiology of PV+ cells. In the present study, we have found that in adult male mice, prefrontocortical PV+ cells surrounded by PNNs show higher density of perisomatic excit…

Male0301 basic medicineInterneuronPrefrontal CortexInhibitory postsynaptic potentialMice03 medical and health sciences0302 clinical medicineInterneuronsBasket cellmedicineExtracellularAnimalsGamma RhythmPrefrontal cortexResearch ArticlesNeuronal PlasticitybiologyChemistryGeneral NeurosciencePerineuronal netExtracellular MatrixMice Inbred C57BLParvalbumins030104 developmental biologymedicine.anatomical_structurenervous systembiology.proteinExcitatory postsynaptic potentialNeuroscience030217 neurology & neurosurgeryParvalbuminThe Journal of Neuroscience
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Effect of Riboflavin/UVA Collagen Cross-linking on Central Cornea, Limbus and Intraocular Pressure. Experimental Study in Rabbit Eyes

2016

The Purpose of present study was to investigate the effect of riboflavin/ultraviolet-A-induced collagen cross-linking (CXL) on central cornea, limbus and intraocular pressure (IOP). This was an animal experimental study. The right corneas of 10 rabbits were ultraviolet-A irradiated (3 mW/cm2 for 30 minutes) after de-epithelialization and instillation of 0.1% riboflavin / 20% Dextran drops. Left corneas served as controls. Samples were examined histologically one month postoperatively. Before and after treatment, IOP measurements were recorded bilaterally. At central cornea of eyes underwent CXL keratocyte repopulation, normal arrangement of collagen fibres and a statistically significant ch…

Male0301 basic medicineIntraocular pressuremedicine.medical_specialtyCollagen cross linkinggenetic structuresUltraviolet RaysIntraocular pressureRiboflavinlcsh:MedicineRiboflavinLimbus CorneaeCornea03 medical and health sciences0302 clinical medicineCollagen fibresOphthalmologyCorneamedicineAnimalsLimbusPhotosensitizing Agentsbusiness.industrylcsh:RSignificant differenceCorneal CrosslinkingGeneral Medicineeye diseasesCross-Linking Reagents030104 developmental biologymedicine.anatomical_structure030221 ophthalmology & optometryCollagenRabbitssense organsCentral corneabusinessAfter treatmentActa Medica (Hradec Kralove, Czech Republic)
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Interference with purinergic signalling

2016

Objective: The association of abacavir (ABC), a guanosine analogue, with cardiovascular toxicity is a long-lasting matter of controversy engendered by the lack of a mechanism of action. Clinical data point to an acute mechanism of vascular inflammation. Previous studies have shown that ABC induces leukocyte-endothelial cell interactions, an indicator of vascular inflammation. These effects are reproduced by another purine analogue, didanosine, but not by pyrimidine or acyclic nucleotide analogues, hinting at an interference with the purinergic system. The aim of the present study was to assess the role of ATP-receptors in leukocyte accumulation induced by ABC. Design and methods: Clinical c…

Male0301 basic medicineIntravital MicroscopyAnti-HIV AgentsImmunologyMacrophage-1 AntigenLeukocyte RollingPharmacologyleukocyte-endothelium interactionsP2X7 receptors03 medical and health sciences0302 clinical medicineIn vivoCell AdhesionLeukocytesmedicineAnimalsHumansImmunology and Allergypurinergic030212 general & internal medicineCell adhesionReceptorCells CulturedMice KnockoutChemistryabacavirPurinergic receptorEndothelial CellsHIVPurinergic signallingDideoxynucleosidescardiovascular diseasesMice Inbred C57BLATP030104 developmental biologyInfectious DiseasesMechanism of actionKnockout mouseReceptors Purinergic P2X7medicine.symptomAIDS
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Nose-to-brain delivery of insulin enhanced by a nanogel carrier.

2018

Recent evidences suggest that insulin delivery to the brain can be an important pharmacological therapy for some neurodegenerative pathologies, including Alzheimer disease (AD). Due to the presence of the Blood Brain Barrier, a suitable carrier and an appropriate route of administration are required to increase the efficacy and safety of the treatment. Here, poly(N-vinyl pyrrolidone)-based nanogels (NG), synthetized by e-beam irradiation, alone and with covalently attached insulin (NG-In) were characterized for biocompatibility and brain delivery features in a mouse model. Preliminarily, the biodistribution of the "empty" nanocarrier after intraperitoneal (i.p.) injection was investigated b…

Male0301 basic medicineIonizing radiationBiodistributionmedicine.medical_treatmentPharmaceutical SciencePharmacologyBrain delivery; Insulin; Intranasal inoculation; Ionizing radiations; Nanogel; Nanogel biocompatibility and clearanceBlood–brain barrierNanogel biocompatibility and clearance03 medical and health sciencesRoute of administrationNanogel0302 clinical medicinemedicineAnimalsHypoglycemic AgentsInsulinProtein kinase BAdministration IntranasalBrain deliveryDrug CarriersChemistryInsulinBrainPovidoneIntranasal inoculationMice Inbred C57BLNasal Mucosa030104 developmental biologymedicine.anatomical_structureAcrylatesNasal administrationSettore CHIM/07 - Fondamenti Chimici Delle TecnologieNanocarriersGels030217 neurology & neurosurgeryNanogel Ionizing radiationNanogel
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Helminth communities of herons (Aves: Ardeidae) in southern Italy

2016

The helminth communities of nine species of herons from southern Italy were studied and compared. Of 24 taxa found including seven digeneans, seven nematodes, six cestodes and four acanthocephalans, only five taxa were found in more than one heron species, and five of the 21 taxa that could be identified to species level were classified as 'heron specialists'. The total number of helminth species per heron species ranged from 1 in Botaurus stellaris to 9 in Ixobrychus minutus with infection levels generally low. A statistical comparison was carried out for herons with a sample size >. 5. At the infracommunity level, only I. minutus clearly differed from other heron species. Diversity parame…

Male0301 basic medicineIxobrychus minutusBotaurusZoologyBirds03 medical and health sciencesBirdAbundance (ecology)Helminthsbiology.animalparasitic diseasesHelminthAnimalsHelminthsbiologyBird DiseasesHost (biology)Ecology030108 mycology & parasitologybiology.organism_classificationInfectious DiseasesTaxonItalyHost specificityFemaleParasitologySpecies richnessHelminthiasis AnimalHeronParasitic worms
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High-elevational occurrence of two tick species, Ixodes ricinus and I. trianguliceps, at their northern distribution range

2021

Abstract Background During the last decades a northward and upward range shift has been observed among many organisms across different taxa. In the northern hemisphere, ticks have been observed to have increased their latitudinal and altitudinal range limit. However, the elevational expansion at its northern distribution range remains largely unstudied. In this study we investigated the altitudinal distribution of the exophilic Ixodes ricinus and endophilic I. trianguliceps on two mountain slopes in Norway by assessing larval infestation rates on bank voles (Myodes glareolus). Methods During 2017 and 2018, 1325 bank voles were captured during the spring, summer and autumn at ten trapping st…

Male0301 basic medicineIxodes ricinusRange (biology)Ixodes ricinus030231 tropical medicineIxodes triangulicepsDistributionTickmedicine.disease_causelcsh:Infectious and parasitic diseases03 medical and health sciencesTicks0302 clinical medicineAltitudeRange shiftIxodes triangulicepsparasitic diseasesMyodes glareolusInfestationmedicineAnimalsVDP::Medisinske Fag: 700lcsh:RC109-216IxodesbiologyArvicolinaeNorwayEcologyResearchAltitudeBank voleRicinusbiology.organism_classificationTick InfestationsBank vole030104 developmental biologyInfectious DiseasesTick-Borne DiseasesFemaleParasitologySeasonsAnimal DistributionParasites & Vectors
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Ranking Series of Cancer-Related Gene Expression Data by Means of the Superposing Significant Interaction Rules Method

2020

The Superposing Significant Interaction Rules (SSIR) method is a combinatorial procedure that deals with symbolic descriptors of samples. It is able to rank the series of samples when those items are classified into two classes. The method selects preferential descriptors and, with them, generates rules that make up the rank by means of a simple voting procedure. Here, two application examples are provided. In both cases, binary or multilevel strings encoding gene expressions are considered as descriptors. It is shown how the SSIR procedure is useful for ranking the series of patient transcription data to diagnose two types of cancer (leukemia and prostate cancer) obtaining Area Under Recei…

Male0301 basic medicineKey genesComputer sciencelcsh:QR1-502Binary numberBiochemistrylcsh:MicrobiologyArticlePattern Recognition AutomatedStructure-Activity Relationship03 medical and health sciencesBig data0302 clinical medicinerankingData MiningHumanscancergene expressionsRelated geneCàncerMolecular BiologyOligonucleotide Array Sequence AnalysisCancerPròstata -- CàncerLeukemiaReceiver operating characteristicbusiness.industryGene Expression ProfilingleukemiaProstatic NeoplasmsLeucèmiaDades massivesPattern recognitionprostate cancerExpressió gènicaSSIR method030104 developmental biologyROC Curvemultilevel fingerprintsExpression dataData Interpretation Statistical030220 oncology & carcinogenesisProstate -- CancerArtificial intelligenceGene expressionbusinessAlgorithms
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Profiles of multidrug-resistant organisms among patients with bacteremia in intensive care units: an international ID-IRI survey

2021

Evaluating trends in antibiotic resistance is a requisite. The study aimed to analyze the profile of multidrug-resistant organisms (MDROs) among hospitalized patients with bacteremia in intensive care units (ICUs) in a large geographical area. This is a 1-month cross-sectional survey for blood-borne pathogens in 57 ICUs from 24 countries with different income levels: lower-middle-income (LMI), upper-middle-income (UMI), and high-income (HI) countries. Multidrug-resistant (MDR), extensively drug-resistant (XDR), or pan-drug-resistant isolates were searched. Logistic regression analysis determined resistance predictors among MDROs. Community-acquired infections were comparable to hospital-acq…

Male0301 basic medicineKlebsiella pneumoniaeDrug ResistanceInfection controlMultidrug resistance0302 clinical medicineMedical microbiologyDrug Resistance Multiple Bacterial80 and overInfection controlMedicine030212 general & internal medicineChildAged 80 and overCross InfectionbiologyMicrobial Sensitivity TestBacterialBacterial InfectionsGeneral MedicineMiddle AgedSciences bio-médicales et agricolesCountriesAnti-Bacterial AgentsAcinetobacter baumanniiEuropeIntensive Care UnitsInfectious DiseasesChild PreschoolFemaleMultipleMicrobiology (medical)Adultmedicine.medical_specialtyAdolescent030106 microbiologyIntensive Care UnitPan-drug resistanceMicrobial Sensitivity TestsInfectionsBacterial InfectionYoung Adult03 medical and health sciencesAntibiotic resistanceIntensive careInternal medicineAnti-Bacterial AgentHumansStewardshipXDRPreschoolAgedCross-Sectional StudieBacteriabusiness.industryInfantbiology.organism_classificationmedicine.diseaseMultiple drug resistanceCross-Sectional StudiesBacteremiabusinessLow- and upper-middle and high income
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