Search results for " Biologia molecolare"
showing 10 items of 267 documents
The implication of MBL deficient haplotypes in acute coronary syndrome
2014
Hbox12: an old gene with a new function.
2013
ENGINEERING OF p65(-1)-/- KNOCK-IN MOUSE, A NEW ALTERNATIVE SPLICED ISOFORM OF p65, A PROTEIN OF NF-KB COMPLEX.
2009
“Caratterizzazione funzionale di p65(-1), nuova isoforma di p65 del complesso NF-kB e preparazione di un clone per l'ingegnerizzazione di un topo p65…
2014
NF-kB è un fattore di trascrizione eucariotico e ubiquitario che regola l’espressione di geni coinvolti in molteplici processi cellulari come la risposta immunitaria, la flogosi, l’apoptosi, la crescita cellulare e lo sviluppo embrionale. Le proteine appartenenti alla famiglia di NF-kB sono p65, c-Rel, RelB, p50 e p52. Tali proteine svolgono la funzione di fattori trascrizionali legando specifiche consensus del DNA (consensus kB) sotto forma di omo - eterodimeri. Ogni membro della famiglia di NF-kB è caratterizzato dalla presenza di un dominio, altamente conservato, il “Rel Homology Domain” (RHD). Nel RHD sono stati mappati i sottodomini di dimerizzazione, interazione con gli inibitori (IkB…
A novel putative interactor for the low density lipoprotein receptor cytoplasmic domain
2011
Familial hypercholesterolemia (FH) is a genetic disease mainly caused by mutations in the low density lipoprotein receptor (LDLLDLLDL-R) gene. However, FH-like phenotypes may also arise from mutations occurring in other genes, the products of which normally interact with the LDLLDLLDL receptor. Although several FH-associated proteins have been discovered, many FH-like phenotypes cannot be linked to mutations in already characterized genes, suggesting the existence of other genes still to be identified, the mutations of which may be directly linked to the FH disorder. In order to identify new putative LDLLDLLDLr interactors possibly involved in its internalization and/or sorting, the cytopla…
Long non-coding RNAs during embryogenesis of the sea urchin
2014
Intriguing studies suggest that long non-coding RNAs (lncRNAs) tend to act in cis with neighboring protein-coding genes. Based on this notion, we assessed whether lncRNAs originate from adjacent genomic regions of selected protein-coding genes, such as early-H2A histone and strim1, that play important functions during development of the sea urchin Paracentrotus lividus. Downregulation of H2A expression relies on the sns5 chromatin insulator placed at the 3'-end of H2A. Northern blot hybridization show that a lncRNA (of ~600 nt), not overlapping with the H2A mRNA, is transcribed from sns5, following H2A expression. The strim1 locus encodes the first echinoderm TRIM-containing factor which fu…
The Value of a Complete Blood Count (CBC) for Sepsis Diagnosis and Prognosis.
2021
Sepsis represents an important global health burden due to its high mortality and morbidity. The rapid detection of sepsis is crucial in order to prevent adverse outcomes and reduce mortality. However, the diagnosis of sepsis is still challenging and many efforts have been made to identify reliable biomarkers. Unfortunately, many investigated biomarkers have several limitations that do not support their introduction in clinical practice, such as moderate diagnostic and prognostic accuracy, long turn-around time, and high-costs. Complete blood count represents instead a precious test that provides a wealth of information on individual health status. It can guide clinicians to early-identify …
PGC-1α: a master gene that is hard to master
2012
Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) is a transcriptional coactivator that favorably affects mitochondrial function. This concept is supported by an increasing amount of data including studies in PGC-1α gene-deleted mice, suggesting that PGC-1α is a rescue factor capable of boosting cell metabolism and promoting cell survival. However, this view has now been called into question by a recent study showing that adeno-associated virus-mediated PGC-1α overexpression causes overt cell degeneration in dopaminergic neurons. How is this to be understood, and can these seemingly conflicting findings tell us something about the role of PGC-1α in cell stress and in cont…