Search results for " Cell"

Rigid Closed‐Cell PUR Foams Containing Polyols Derived from Renewable Resources: The Effect of Polymer Composition, Foam Density, and Organoclay Fill…

2017

Validation of HF radar sea surface currents in the Malta-Sicily Channel

2019

Abstract A network of High-Frequency radar (HFR) stations runs operationally in the Malta-Sicily Channel (MSC), Central Mediterranean Sea, providing sea surface current maps with high temporal (1 h) and spatial (3 × 3 km) resolutions since August 2012. Comparisons with surface drifter data and near-surface Acoustic Doppler Current Profiler (ADCP) observations, as well as radar site-to-site baseline analyses, provide quantitative assessments of HFR velocities accuracy. Twenty-two drifters were deployed within the HFR domain of coverage between December 2012 and October 2013. Additionally, six ADCP vertical current profiles were collected at selected positions during a dedicated field survey.…

The high overtone and combination levels of SF6 revisited at Doppler-limited resolution: A global effective rovibrational model for highly excited vi…

2017

Abstract Sulfur hexafluoride is an important prototypal molecule for modeling highly excited vibrational energy flow and multi quanta absorption processes in hexafluoride molecules of technological importance. It is also a strong greenhouse gas of anthropogenic origin. This heavy species, however, features many hot bands at room temperature (at which only 30% of the molecules lie in the ground vibrational state), especially those originating from the lowest, v 6 =1 vibrational state. Using a cryogenic long path cell with variable optical path length and temperatures regulated between 120 and 163 K, coupled to Synchrotron Radiation and a high resolution interferometer, Doppler-limited spectr…

Bodegas, lagares y almazaras en el territorio de Kelin (siglos V-III a. C.): el caso de la Rambla de la Alcantarilla (Requena, Valencia)

2016

Archaeological evidences date the expansion of grape vineyards in the territory of the Iberian Iron Age city of Kelin from the 5th to 3rd centuries BC; although the origins are documented in the 7th BC. One of the singularities of this territory is the existence of outdoors stone structures for the production of wine and oil. All are located in a specific area of the territory (la Alcantarilla and Los Morenos ravines) (Requena, Valencia), and are associated with archaeological materials that allow date them as Iberian. In this paper we approach the rural settlement pattern in la Alcantarilla ravine and we present in detail the archaeological sites of Rambla de la Alcantarilla and Solana de …

Improvement of osteogenic differentiation of human mesenchymal stem cells on composite poly l-lactic acid/nano-hydroxyapatite scaffolds for bone defe…

2020

Tissue engineering offers new approaches to repair bone defects, which cannot be repaired physiologically, developing scaffolds that mimic bone tissue architecture. Furthermore, biomechanical stimulation induced by bioreactor, provides biomechanical cues that regulate a wide range of cellular events especially required for cellular differentiation and function. The improvement of human mesenchymal stem cells (hMSCs) colonization in poly-L-lactic-acid (PLLA)/nano- hydroxyapatite (nHA) composite scaffold was evaluated in terms of cell proliferation (dsDNA content), bone differen- tiation (gene expression and protein synthesis) and ultrastructural analysis by comparing static (s3D) and dynamic…

Give me a sample of air and I will tell which species are found from your region : Molecular identification of fungi from airborne spore samples

2017

Fungi are a megadiverse group of organisms, they play major roles in ecosystem functioning and are important for human health, food production and nature conservation. Our knowledge on fungal diversity and fungal ecology is however still very limited, in part because surveying and identifying fungi is time demanding and requires expert knowledge. We present a method that allows anyone to generate a list of fungal species likely to occur in a region of interest, with minimal effort and without requiring taxonomical expertise. The method consists of using a cyclone sampler to acquire fungal spores directly from the air to an Eppendorf tube, and applying DNA barcoding with probabilistic specie…

C4-like photosynthesis and the effects of leaf senescence on C4-like physiology in Sesuvium sesuvioides (Aizoaceae).

2019

Sesuvium sesuvioides represents a young C4 lineage with C4-like metabolism: CO2 compensation points range between C4 and C3–C4 intermediate values, and Rubisco was detected in bundle sheath and mesophyll.

Ultraviolet radiation accelerates photodegradation under controlled conditions but slows the decomposition of senescent leaves from forest stands in …

2019

Depending on the environment, sunlight can positively or negatively affect litter decomposition, through the ensemble of direct and indirect processes constituting photodegradation. Which of these processes predominate depends on the ecosystem studied and on the spectral composition of sunlight received. To examine the relevance of photodegradation for litter decomposition in forest understoreys, we filtered ultraviolet radiation (UV) and blue light from leaves of Fagus sylvatica and Bettda pendula at two different stages of senescence in both a controlled-environment experiment and outdoors in four different forest stands (Picea abies, Pagus sylvatica, Acer platanoides, Betula pendula). Co…

Role of Bacillus thuringiensis Cry1A toxins domains in the binding to the ABCC2 receptor from Spodoptera exigua

2018

Abstract Cry proteins from Bacillus thuringiensis (Bt) have been used to control insect pests either as formulated sprays or as in Bt-crops. However, field-evolved resistance to Bt proteins is threatening the long-term use of Bt products. The SeABCC2 locus has been genetically linked to resistance to a Bt bioinsecticide (Xentari™) in Spodoptera exigua (a mutation producing a truncated form of the transporter lacking an ATP binding domain was found in the resistant insects). Here, we investigated the role of SeABCC2 in the mode of action of Cry1Aa, Cry1Ab, Cry1Ac, Cry1Ca, and two Cry1A-1Ca hybrids by expressing the receptor in Sf21 and HEK293T cell lines. Cell toxicity assays showed that Sf2…

Proliferation of aneuploid cells induced by CENP-E depletion is counteracted by the p14ARF tumor suppressor

2018

The spindle assembly checkpoint (SAC) is a cellular surveillance mechanism that ensures the fidelity of chromosomes segregation. Reduced expression of some of its components weakens the SAC and induces chromosome instability and aneuploidy, which are both well-known hallmarks of cancer cells. Centromere protein-E (CENP-E) is a crucial component of the SAC and its function is to facilitate kinetochore microtubule attachment required to achieve and maintain chromosome alignment. The present study investigates the possible role of p14ARF as a controller of aneuploid cells proliferation. We used RNA interference to induce aneuploidy by partial depletion of CENP-E in human primary fibroblasts (I…