Search results for " Cellular"

Generating Multi State Cellular Automata by using Chua’s ”Universal Neuron”

2007

Halloysite nanotubes-carbon dots hybrids multifunctional nanocarrier with positive cell target ability as a potential non-viral vector for oral gene …

2019

Abstract Hypothesis The use of non-viral vectors for gene therapy is hindered by their lower transfection efficiency and their lacking of self-track ability. Experiments This study aims to investigate the biological properties of halloysite nanotubes-carbon dots hybrid and its potential use as non-viral vector for oral gene therapy. The morphology and the chemical composition of the halloysite hybrid were investigated by means of high angle annular dark field scanning TEM and electron energy loss spectroscopy techniques, respectively. The cytotoxicity and the antioxidant activity were investigated by standard methods (MTS, DPPH and H2O2, respectively) using human cervical cancer HeLa cells …

Survival Strategy of Erwinia amylovora against Copper: Induction of the Viable-but-Nonculturable State

2006

Copper compounds, widely used to control plant-pathogenic bacteria, have traditionally been employed against fire blight, caused by Erwinia amylovora. However, recent studies have shown that some phytopathogenic bacteria enter into the viable-but-nonculturable (VBNC) state in the presence of copper. To determine whether copper kills E. amylovora or induces the VBNC state, a mineral medium without copper or supplemented with 0.005, 0.01, or 0.05 mM Cu2+ was inoculated with 107 CFU/ml of this bacterium and monitored over 9 months. Total and viable cell counts were determined by epifluorescence microscopy using the LIVE/DEAD kit and by flow cytometry with 5-cyano-2,3-ditolyl tetrazolium chlori…

Dynamic regulation of the cancer stem cell compartment by Cripto-1 in colorectal cancer.

2015

Stemness was recently depicted as a dynamic condition in normal and tumor cells. We found that the embryonic protein Cripto-1 (CR1) was expressed by normal stem cells at the bottom of colonic crypts and by cancer stem cells (CSCs) in colorectal tumor tissues. CR1-positive populations isolated from patient-derived tumor spheroids exhibited increased clonogenic capacity and expression of stem-cell-related genes. CR1 expression in tumor spheroids was variable over time, being subject to a complex regulation of the intracellular, surface and secreted protein, which was related to changes of the clonogenic capacity at the population level. CR1 silencing induced CSC growth arrest in vitro with a …

On closures of discrete sets

2018

The depth of a topological space $X$ ($g(X)$) is defined as the supremum of the cardinalities of closures of discrete subsets of $X$. Solving a problem of Mart\'inez-Ruiz, Ram\'irez-P\'aramo and Romero-Morales, we prove that the cardinal inequality $|X| \leq g(X)^{L(X) \cdot F(X)}$ holds for every Hausdorff space $X$, where $L(X)$ is the Lindel\"of number of $X$ and $F(X)$ is the supremum of the cardinalities of the free sequences in $X$.

Competition for resources modulates cell-mediated immunity and stress hormone level in nestling collared doves (Streptopelia decaocto)

2008

International audience; Competitive stress imposed by hatching asynchrony may affect developmental trajectories of offsprings by regulating resource allocation between growth and other fitness-related traits. For instance, the down-regulation of immunity is a commonly observed phenomenon under stressful conditions. However, physiological mechanisms that regulate resources allocation to growth and immune functions in response to competition for resources, as well as inter-sexual differences in physiological strategies, are still poorly investigated. To partially fill this gap, we first conducted a descriptive study on chicks of the collared dove (Streptopelia decaocto), a species producing t…

Parallelization of Cellular Automata for Surface Reactions

2002

We present a parallel implementation of cellular automata to simulate chemical reactions on surfaces. The scaling of the computer time with the number of processors for this parallel implementation is quite close to the ideal T/P, where T is the computer time used for one single processor and P the number of processors. Two examples are presented to test the algorithm, the simple A+B->0 model and a realistic model for CO oxidation on Pt(110). By using large parallel simulations, it is possible to derive scaling laws which allow us to extrapolate to even larger system sizes and faster diffusion coefficients allowing us to make direct comparisons with experiments.

Association between DRD2/DRD4 interaction and conduct disorder: a potential developmental pathway to alcohol dependence

2013

1 Department of Genetics, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil 2 King’s College London, Institute of Psychiatry, MRC Social Genetic and Developmental Psychiatry Centre, London, United Kingdom, UK 3 Department of Child and Adolescent Psychiatry and Psychotherapy, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany 4 Department of Cognitive Neuroscience, Donders Institute for Brain, Cognition and Behavior, Radboud University Medical Center, Nijmegen, The Netherlands 5 Psychology Department, National University of Singapore, Singapore, Singapore 6 Department of Psychiatry, Donders Institute for Brain, Cognition an…

Vers des indicateurs locaux de performance énergétique : les étiquettes énergétiques territoriales. Une première approche à partir de l'estimation de…

2009

Global warming compels to investigate the decrease of green house gas effect emissions and so energy consumption reduction. At local scale, it leads to reach concrete goals regarding energy efficiency, which could be approached through the definition of indicators and guidelines for decision-makers. From the example of the city of Besançon (France), this research aims to define an original indicator, useful for planners. Considering a standardized mobility behaviour of inhabitants, we propose elements of methodology to measure the relative energy consumption of each zone based on their relative distance to the nearest shops, services and employment zones. The results display "zonal energy l…

Test system for trifunctional antibodies in 3D MCTS culture.

2009

The aim of the present study was to assess the feasibility of a 3D tumor cell culture model, that is, multicellular tumor spheroids (MCTSs) as an adequate model for micrometastases and therefore as a pharmacological model for efficacy testing of trifunctional therapeutic antibodies. Unlike conventional monolayer cultures, spheroids allow researchers to study parameters, such as 3D cell shape, 3D cell arrangement and microenvironment, and penetration efficiency of defense cells that may largely influence the efficacy of antibody treatment in vivo. The authors established a long-term coculture of human MCTSs with peripheral blood mononuclear cells (PBMCs) to test the anticancer effect of the …