Search results for " Cult"
showing 10 items of 9649 documents
Protein phosphorylation is induced in tobacco cells by the elicitor cryptogein
1994
Changes in plasmalemma ion fluxes were observed when tobacco (Nicotiana tabacum) cells were treated with cryptogein, a proteinaceous elicitor from Phytophthora cryptogea. A strong alkalization of the culture medium, accompanied by a leakage of potassium, was induced within a few minutes of treatment. These effects reached a maximum after 30 to 40 min and lasted for several hours. This treatment also resulted in a rapid, but transient, production of activated oxygen species. All these physiological responses were fully sensitive to staurosporine, a known protein kinase inhibitor. Furthermore, a study of protein phosphorylation showed that cryptogein induced a staurosporine-sensitive phosphor…
Nuclear rDNA instability in in vitro-generated plants is amplified after sexual reproduction with conspecific wild individuals
2016
Using micropropagation through tissue culture has become the most used approach worldwide for mass production for the conservation of endangered species. However, the screening of somaclonal variations generated using in vitro culture is usually restricted to the first generation of micropropagated plants, when they have not yet been released in the field. Accordingly, the fate of genetically modified regenerants after sexual reproduction is usually not assessed and changes in the genetic structures of species are unknown. In this work, we assess the cytogenetic stability of two rDNA gene families in the offspring of experimental crosses between accessions generated after in vitro culture a…
Floral Color, Anthocyanin Synthesis Gene Expression and Control in Cape Erica Species
2019
Introduction: The Cape Floristic Region (CFR) is a biodiversity hotspot, recognized globally for its unusually high levels of endemism. The origins of this biodiversity are a long-standing topic of research. The largest “Cape clade,” Erica, radiated dramatically in the CFR, its ca. 690 species arising within 10–15 Ma. Notable between- and within-species flower color variation in Erica may have contributed to the origins of species diversity through its impact on pollinator efficiency and specificity. Methods: We investigate the expression and function of the genes of the anthocyanin biosynthesis pathway that controls floral color in 12 Erica species groups using RT-qPCR and UPLC-MS/MS. Resu…
Transcriptional responses of Medicago truncatula upon sulfur deficiency stress and arbuscular mycorrhizal symbiosis
2014
International audience; Sulfur plays an essential role in plants' growth and development and in their response to various abiotic and biotic stresses despite its leachability and its very low abundance in the only form that plant roots can uptake (sulfate). It is part of amino acids, glutathione (GSH), thiols of proteins and peptides, membrane sulfolipids, cell walls and secondary products, so reduced availability can drastically alter plant growth and development. The nutritional benefits of symbiotic interactions can help the plant in case of S deficiency. In particular the arbuscular mycorrhizal (AM) interaction improves N, P and S plant nutrition, but the mechanisms behind these exchang…
Long-term mineral fertiliser use and maize residue incorporation do not compensate for carbon and nutrient losses from a Ferralsol under continuous m…
2015
9 pages; International audience; It has been repeatedly argued that mineral fertiliser application combined with in situ retention of crop residue biomass can sustain long-term productivity of West African soils. Using 20-year experimental data from southern Togo, a biannual rainfall area, we analysed the effect of two rates of mineral NPK fertiliser application to maize–cotton rotation on the long-term dynamics of soil C and nutrient contents, as compared with two control treatments. Mineral fertiliser treatments consisted of application to both maize (first season) and cotton (second season) the research-recommended NPK rates (Fertiliser-RR) and 1.5 times these rates (Fertiliser-1.5 RR). …
Reducing the effect of beauvericin on neuroblastoma SH-SY5Y cell line by natural products
2020
Abstract In the present work, different natural compounds from coffee by-product extracts (coffee silverskin and spent coffee) rich in polyphenols, was investigated against beauvericin (BEA) induced-cytotoxicity on SH-SY5Y cells. Spent coffee arise as waste products through the production of instant coffee and coffee brewing; while the silverskin is a tegument which is removed and eliminated with toasting coffee grains. First of all, polyphenol extraction methods, measurement of total polyphenols content and its identification were carried out. Afterwards evaluating in vitro effects with MTT assay on SH-SY5Y cells of coffee by-product extracts and mycotoxins at different concentrations and …
Use of biochar as peat substitute for growing substrates of Euphorbia × lomi potted plants
2016
Biochar from conifers wood was used in soilless culture as growing substrate alternative to peat for ornamental crops. Potted plants of Euphorbia × lomi Rauh cv. ‘Ilaria’ were grown with different mixtures (v:v) of brown peat and biochar in order to evaluate main physical and chemical characteristics of this biomaterial as well as its effect on plant growth, ornamental characteristics and nutrients uptake. Biochar addition to peat increased pH, EC and K content of the growing substrates, as well as air content and bulk density. Biochar content of substrates significantly affected plant growth and biomass partitioning: higher number of shoots and leaves, leaf area and leaf dry weight were re…
The role of noise on the steady state distributions of phytoplankton populations
2016
The spatio-temporal behaviour of total chlorophyll concentration is investigated in the middle of the Tyrrhenian Sea by using a stochastic approach. The study is based on a reaction-diffusion-taxis model, which is used to analyse the dynamics of five phytoplankton groups, responsible for about 80% of the total chlorophyll a inside the euphotic zone of the water column. The analysis is performed by considering: (i) the intraspecific competition of the phytoplanktonic groups for limiting factors, i.e. light intensity and nutrient concentration, (ii) the seasonal changes of environmental variables, and (iii) the random fluctuations of the components of the velocity field and temperature. Speci…
The WtmsDW Locus on Wheat Chromosome 2B Controls Major Natural Variation for Floret Sterility Responses to Heat Stress at Booting Stage
2021
Heat stress at booting stage causes significant losses to floret fertility (grain set) and hence yield in wheat (Triticum aestivum L.); however, there is a lack of well-characterized sources of tolerance to this type of stress. Here, we describe the genetic analysis of booting stage heat tolerance in a cross between the Australian cultivars Drysdale (intolerant) and Waagan (tolerant), leading to the definition of a major-effect tolerance locus on the short arm of chromosome 2B, Wheat thermosensitive male sterile Drysdale/Waagan (WtmsDW). WtmsDW offsets between 44 and 65% of the losses in grain set due to heat, suggesting that it offers significant value for marker-assisted tolerance breedin…
Fruit and Leaf Sensing for Continuous Detection of Nectarine Water Status
2019
Continuous assessment of plant water status indicators provides the most precise information for irrigation management and automation, as plants represent an interface between soil and atmosphere. This study investigated the relationship of plant water status to continuous fruit diameter (FD) and inverse leaf turgor pressure rates (pp) in nectarine trees [Prunus persica (L.) Batsch] throughout fruit development. The influence of deficit irrigation treatments on stem (Ψstem) and leaf water potential, leaf relative water content, leaf stomatal conductance, and fruit growth was studied across the stages of double-sigmoidal fruit development in ‘September Bright’ nectarines. Fruit relative grow…