6533b855fe1ef96bd12aff5a

RESEARCH PRODUCT

Reducing the effect of beauvericin on neuroblastoma SH-SY5Y cell line by natural products

Gaia De SimoneCristina JuanGianni SagratiniAna Juan-garcíaJordi MañesGiovanni Caprioli

subject

0106 biological sciencesSH-SY5YToxicologyCoffee01 natural sciencesAntioxidantsNeuroblastoma03 medical and health scienceschemistry.chemical_compoundChlorogenic acidCell Line TumorDepsipeptidesHumansMTT assayFood scienceMycotoxinBiological Products0303 health sciencesPlant ExtractsChemistrybusiness.industry010604 marine biology & hydrobiology030302 biochemistry & molecular biologyfood and beveragesMycotoxinsBeauvericinCell culturePolyphenolBrewingbusiness

description

Abstract In the present work, different natural compounds from coffee by-product extracts (coffee silverskin and spent coffee) rich in polyphenols, was investigated against beauvericin (BEA) induced-cytotoxicity on SH-SY5Y cells. Spent coffee arise as waste products through the production of instant coffee and coffee brewing; while the silverskin is a tegument which is removed and eliminated with toasting coffee grains. First of all, polyphenol extraction methods, measurement of total polyphenols content and its identification were carried out. Afterwards evaluating in vitro effects with MTT assay on SH-SY5Y cells of coffee by-product extracts and mycotoxins at different concentrations and exposure times was performed. TPC in silverskin coffee by-product extracts was >10 times higher than in spent coffee by-product extracts. Chlorogenic acid was the majority polyphenol detected. Viability for BEA reached IC50 values at 72h (2.5 μM); boiling water silverskin coffee extract reached the highest viability also in pre-treatment BEA exposure and compared with MeOH and MeOH:H2O (v/v, 50:50) extracts. These results in SH-SY5Y cells highlight the use of such residues as supplements or bioactive compounds in the future.

yearjournalcountryeditionlanguage
2020-08-27Toxicon
https://doi.org/10.1016/j.toxicon.2020.10.017