Search results for " DNA"
showing 10 items of 2475 documents
Investigation and control of a Norovirus outbreak of probable waterborne transmission through a municipal groundwater system
2014
During March 2011 an outbreak of gastroenteritis occurred in Santo Stefano di Quisquina, Agrigento, Sicily, Italy. Within two weeks 156 cases were identified among the 4,965 people living in the municipality. An epidemiological investigation was conducted to characterize the outbreak and target the control measures. A case was defined as a person developing diarrhea or vomiting during February 27–March 13, 2011. Stool specimens were collected from 12 cases. Norovirus (NoV) genotype GII.4 variant New Orleans 2009 was identified in stool samples from 11 of 12 cases tested (91.7%). Epidemiological investigations suggested a possible association with municipal drinking water consumption. Water …
Senās DNS analīžu pielietojums Latvijas teritorijas dzelzs laikmeta apbedījumu raksturojumā
2022
Darba mērķis: analizēt Latvijas teritorijā iegūto arheoloģisko osteoloģisko materiālu, veicot senās DNS analīzi, lai novērtētu endogēnās DNS saglabātības pakāpi un paplašinātu esošās zināšanas par Daugavas un Mēmeles krastus apdzīvojušo iedzīvotāju ģenētisko izcelsmi. Tika veikta mtDNS haplogrupu sastopamības izpēte senās DNS paraugiem, gan izmantojot publicētus rezultātus, gan veicot senās DNS analīzi dzelzs laikmeta paraugiem. Dzelzs laikmetā Latvijas teritorijā konstatējamas jaunas mtDNS haplogrupas, kas sakrīt ar līdzīgi datētām arī citur Baltijas jūras reģionā. Pētījumā izmantotās metodes ir efektīvas, tomēr nelielais mtDNS haplogrupu rezultātu skaits var tikt saistīts ar zemu senās DN…
Palaeogenetics and cultural heritage. Species determination and STR-genotyping from ancient DNA in art and artefacts
2000
Abstract In recent years, a few papers have addressed the palaeogenetic analysis of cultural, historical and archaeological artefacts. We provide an overview of the individual published articles and then describe the results we had in the framework of a palaeogenetic research project involving various historical and prehistoric finds from museums, archaeological excavations, and libraries. We show that ancient DNA can be isolated from most of the various biomaterials (leather, parchment, glue, binding media, crusted organic plant remains in containers). Short pieces of degraded DNA are used, on the one hand, to determine the organic remnant’s genus/species of origin, and on the other hand, …
Population differentiation of the European pond turtle (Emys orbicularis) in Poland inferred by the analysis of mitochondrial and microsatellite DNA …
2013
We investigated the genetic diversity of Polish populations of the European pond turtle (Emys orbicularis) using complete sequences of the mitochondrial cytochrome b gene and allelic variation at thirteen microsatellite loci. We collected data from 146 turtles from 28 locations covering most of the species’ range in Poland. Our results showed a low haplotype diversity and high levels of microsatellite diversity in all populations. We applied two Bayesian approaches using the multilocus data and determined relationships of mtDNA haplotypes by constructing a parsimony network. We observed relatively consistent results of the two Bayesian clustering methods and largely concordant differentiati…
A population genetics view of animal domestication
2012
The fundamental shift associated with the domestication of plants and animals allowed for a dramatic increase in human population sizes and the emergence of modern society. Despite its importance and the decades of research devoted to studying it, questions regarding the origins and processes of domestication remain. Here, we review recent theoretical advances and present a perspective that underscores the crucial role that population admixture has played in influencing the genomes of domestic animals over the past 10000 years. We then discuss novel approaches to generating and analysing genetic data, emphasising the importance of an explicit hypothesis-testing approach for the inference of…
Evidence from mtDNA RFLP analysis for the introduction of Fundulus heteroclitus to southwestern Spain
1996
Restriction analysis of mitochondrial DNA confirmed the taxonomic status of a southwestern Iberian Peninsula population of Fundulus heteroclitus and its probable origin in North America.
Ancient mitochondrial DNA analyses of Iberian sturgeons
2009
Summary Today’s European sturgeons are relics of erstwhile widely distributed populations, diminished mainly by overfishing and habitat changes over the centuries. While extinct European populations in the Baltic and North seas have been identified as Acipenser oxyrhinchus or A. sturio, a clear species determination on the Iberian Peninsula is still lacking. Plans to conserve existing populations and to re-introduce extinct wild populations in European rivers will benefit from information of historic population/genotype composition. In this study, we used techniques involving ancient DNA as well as morphological comparisons based on bony scutes to identify twelve samples from five archaeolo…
Response to Comment on "Ancient DNA from the First European Farmers in 7500-Year-Old Neolithic Sites"
2006
The discovery of mitochondrial type N1a in Central European Neolithic skeletons at a high frequency enabled us to answer the question of whether the modern population is maternally descended from the early farmers instead of addressing the traditional question of the origin of early European farmers.
Set Membership (In) Validation of nonlinear positive models for biological systems
2006
The complexity of biology needs quantitative tools in order to support and validate biologists intuition and traditional qualitative descriptions. In this paper, Nonlinear Positive models with constraints for biological systems are validated/invalidated in a worst-case deterministic setting. These models are usefull for the analysis of the DNA and RNA evolution and for the description of the population dynamics of viruses and bacteria. The conditional central estimate and the Uncertainty Intervals are determined in order to validate/invalidate the model. The effectiveness of the proposed procedure has been illustrated by means of simulation experiments.
Recombinant GM-CSF Induces in Vitro Differentiation of Dendritic Cells from Mouse Bone Marrow
1993
The unprecedented functional capacity of dendritic cells (DC) in sensitizing resting T cells and their role in triggering T dependent immune responses attract increasing interest in this unique accessory cell population. Like macrophages (Mph) DC have been described to originate in the bone marrow (BM) (1). While the cytokine-promoted in vitro differentiation of Mph from BM-cells is well established, a convincing in vitro culture system for propagating mouse DC from BM-cells has not yet been reported. This work demonstrates the differentiation of DC from mouse bone marrow cells by a short term in vitro culture system supplemented with rGM-CSF.