Search results for " DUP"
showing 10 items of 249 documents
Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes
2021
Nucleic acids are essential biomolecules in living systems and represent one of the main targets of chemists, biophysics, biologists, and nanotechnologists. New small molecules are continuously developed to target the duplex (ds) structure of DNA and, most recently, RNA to be used as therapeutics and/or biological tools. Stimuli-triggered systems can promote and hamper the interaction to biomolecules through external stimuli such as light and metal coordination. In this work, we report on the interaction with ds-DNA and ds-RNA of two aza-macrocycles able to coordinate Zn2+ metal ions and form binuclear complexes. The interaction of the aza-macrocycles and the Zn2+ metal complexes with duple…
Eight million years of maintained heterozygosity in chromosome homologs of cercopithecine monkeys
2020
In the Cercopithecini ancestor two chromosomes, homologous to human chromosomes 20 and 21, fused to form the Cercopithecini specific 20/21 association. In some individuals from the genus Cercopithecus, this association was shown to be polymorphic for the position of the centromere, suggesting centromere repositioning events. We set out to test this hypothesis by defining the evolutionary history of the 20/21 association in four Cercopithecini species from three different genera. The marker order of the various 20/21 associations was established using molecular cytogenetic techniques, including an array of more than 100 BACs. We discovered that five different forms of the 20/21 association w…
The ideal duplication
2021
AbstractIn this paper we present and study the ideal duplication, a new construction within the class of the relative ideals of a numerical semigroup S, that, under specific assumptions, produces a relative ideal of the numerical duplication $$S\bowtie ^b E$$ S ⋈ b E . We prove that every relative ideal of the numerical duplication can be uniquely written as the ideal duplication of two relative ideals of S; this allows us to better understand how the basic operations of the class of the relative ideals of $$S\bowtie ^b E$$ S ⋈ b E work. In particular, we characterize the ideals E such that $$S\bowtie ^b E$$ S ⋈ b E is nearly Gorenstein.
Computation of Yvon-Villarceau circles on Dupin cyclides and construction of circular edge right triangles on tori and Dupin cyclides
2014
Ring Dupin cyclides are non-spherical algebraic surfaces of degree four that can be defined as the image by inversion of a ring torus. They are interesting in geometric modeling because: (1) they have several families of circles embedded on them: parallel, meridian, and Yvon-Villarceau circles, and (2) they are characterized by one parametric equation and two equivalent implicit ones, allowing for better flexibility and easiness of use by adopting one representation or the other, according to the best suitability for a particular application. These facts motivate the construction of circular edge triangles lying on Dupin cyclides and exhibiting the aforementioned properties. Our first contr…
Blending pieces of Dupin cyclides for 3D modeling and reconstruction : study in the space of spheres
2013
The thesis deals with the blending of canal surfaces in geometric modeling using pieces of Dupin Cyclides. We try to solve a problem of reconstructing real parts manufactured and controlled by the CEA of Valduc. Using the space of spheres in which we can manipulate both points, spheres and canal surfaces, we simplify some problems. This space is represented by a 4-dimensional quadric in a 5-dimensional space, equipped with the Lorentz form, it is the Lorentz space. In the space of spheres, problems of blending canal surfaces by pieces of Dupin cyclides are simplified in linear problems. We give algorithms to make such blends using the space of spheres and after we come back to 3 dimensions …
The DNA-binding subunit p140 of replication factor C is upregulated in cycling cells and associates with G 1 phase cell cycle regulatory proteins
1999
The DNA-binding subunit of replication factor C (RFCp140) plays an important role in both DNA replication and DNA repair. The mechanisms regulating activation of RFCp140 thereby controlling replication and cellular proliferation are largely unknown. We analyzed protein expression of RFCp140 during cell cycle progression and investigated the association of RFCp140 with cell cycle regulatory proteins in cell lines of various tissue origin and in primary hematopoietic cells. Western and Northern blot analyses of RFCp140 from synchronized cells showed downregulation of RFCp140 when cells enter a G0-like quiescent state and upregulation of RFCp140 in cycling cells. Translocation from the cytopla…
Preservation of genetic and regulatory robustness in ancient gene duplicates of Saccharomyces cerevisiae
2014
[EN] Biological systems remain robust against certain genetic and environmental challenges. Robustness allows the exploration of ecological adaptations. It is unclear what factors contribute to increasing robustness. Gene duplication has been considered to increase genetic robustness through functional redundancy, accelerating the evolution of novel functions. However, recent findings have questioned the link between duplication and robustness. In particular, it remains elusive whether ancient duplicates still bear potential for innovation through preserved redundancy and robustness. Here we have investigated this question by evolving the yeast Saccharomyces cerevisiae for 2200 generations …
The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants
2008
We report the draft genome sequence of the model moss Physcomitrella patens and compare its features with those of flowering plants, from which it is separated by more than 400 million years, and unicellular aquatic algae. This comparison reveals genomic changes concomitant with the evolutionary movement to land, including a general increase in gene family complexity; loss of genes associated with aquatic environments (e.g., flagellar arms); acquisition of genes for tolerating terrestrial stresses (e.g., variation in temperature and water availability); and the development of the auxin and abscisic acid signaling pathways for coordinating multicellular growth and dehydration response. The …
Replication origins and pause sites in sea urchin mitochondrial DNA
1992
We have used a combination of one- and two-dimensional agarose gel electrophoresis, and solution hybridization to strand-specific probes, to map the replication origin of sea urchin mitochondrial DNA and to investigate the structure of replication intermediates. These assays are consistent with replication initiating unidirectionally from the D-loop region by D-loop expansion, as in vertebrates. A prominent site of initiation of lagging-strand synthesis lies at, or near to, the boundary between the genes for ATPase 6 and COIII, which is also close to a pause site for leading-strand synthesis. These findings suggest a role for pause sites in the regulation of mitochondrial transcription and …
Multiple roles for ISWI in transcription, chromosome organization and DNA replication.
2003
ISWI functions as the ATPase subunit of multiple chromatin-remodeling complexes. These complexes use the energy of ATP hydrolysis to slide nucleosomes and increase chromatin fluidity, thereby modulating the access of transcription factors and other regulatory proteins to DNA. Here we discuss recent progress toward understanding the biological functions of ISWI, with an emphasis on its roles in transcription, chromosome organization and DNA replication.