Search results for " Extraction"
showing 10 items of 1344 documents
Standardization of islet isolation outcome- A new automatic system to determine pancreatic islet viability
2011
Pancreatic islet transplantation is emerging as a therapeutic approach for patients affected by diabetes. This approach consists of a minimally invasive procedure replacing insulin-producing cells (pancreatic islets). The technique has been proven successful, but limitations have been identified. One of the major challenges of the procedure is the counting of the isolated pancreatic islets, which is currently jeopardized by subjectivity and inaccuracy. Determination of the accurate islet number is a crucial factor in determining the correlation between the isolation product and clinical outcome. In the proposed study, we have developed software capable of objectively evaluating islet number…
Optimisation of Conditions for Extraction of Biologically Active Compounds from Common Bryophytes in Latvia / Latvijâ Augoðu Briofîtu Ekstrakcijas Ap…
2015
Abstract Bryophytes are the second largest taxonomic group in the plant kingdom. They contain a high number of biologically active compounds. Studies of their composition are important for understanding evolutionary processes in the plant kingdom. The aim of this study was to assess bryophyte secondary metabolite extraction options and to increase the yields of polyphenols and substances determining the free radical scavenging activity of bryophyte extracts. Similar studies have been conducted using higher plants as model organisms, but not using bryophytes. Comparison of five extraction methods (conventional, Soxhlet extraction, treatment with microwaves, ultrasound, and supercritical CO2 …
A Curvature Based Method for Blind Mesh Visual Quality Assessment Using a General Regression Neural Network
2016
International audience; No-reference quality assessment is a challenging issue due to the non-existence of any information related to the reference and the unknown distortion type. The main goal is to design a computational method to objectively predict the human perceived quality of a distorted mesh and deal with the practical situation when the reference is not available. In this work, we design a no reference method that relies on the general regression neural network (GRNN). Our network is trained using the mean curvature which is an important perceptual feature representing the visual aspect of a 3D mesh. Relatively to the human subjective scores, the trained network successfully asses…
Pulsed electric field (PEF) as an efficient technology for food additives and nutraceuticals development
2020
Abstract The use of pulsed electric field (PEF) technology has been extensively investigated in the area of food pasteurization and gene transfection. In the recent years the technology has also shown promising application in the development of various plant- and microorganism-based added-value compounds. The main working principle of PEF is electroporation or electropermeabilization of cell membranes. The pretreatment of biological tissues with PEF improves mass transfer during solvent extraction of metabolites and thereby increases the extraction efficiency. Moreover, the application of PEF at lower intensity has shown to induce stress on the biological tissues that in turn improves the a…
High Resolution-magic Angle Spinning NMR Study of Olive Leaves
2013
In recent years HR-MAS 1H NMR spectroscopy has proven to be a useful tool for the rapid determination of the metabolic profile of several solid and semisolid foods, such as fruits and vegetables, cheese and meat. Olive leaves are today recognized as direct sources of bioactive compounds and natural antioxidants (flavonoids, secoiridoids) suitable as food additives, and their extracts form the basis of beauty care products and pharmaceutical supplements. Thus, olive leaves are emerging as a new and potentially important product for olive tree growing regions. In this report we present the application of 1H and 13C HR-MAS 1D and 2D NMR spectroscopy for the characterization and analysis of tri…
New Method of DNA Isolation from Two Food Additives Suitable for Authentication in Polymerase Chain Reaction Assays
2005
Locust bean gum and guar gum are galactomannans used as additives (E 410 and E 412, respectively) in the food industry as stabilizing agents. Analytical discrimination between the two additives in gums and foods is now feasible by molecular techniques. However, only complex and time-consuming DNA isolation protocols are available to date. We have developed simple improved protocols to obtain enough DNA suitable for PCR amplification from a few milligrams of commercial E 410 and E 412 additives (containing more than 75% polysaccharides). The suspension of additives in water or 10 mM Tris-HCl, pH 8.5, efficiently recovers DNA suitable for authentication in PCR assays. However, the Tris method…
An overview of the traditional and innovative approaches for pectin extraction from plant food wastes and by-products: Ultrasound-, microwaves-, and …
2018
Abstract Background A large amount of food wastes and by-products are produced from farm to plate. They represent valuable sources for the production of high-added value compounds such as pectin. Pectin is the methylated ester of polygalacturonic acid and presents a wide range of applications in pharmaceutical and cosmetic products as well as in food industry such as gelling agent in fruit-based products, stabilizer in fruit and milk beverages and fruit filling for bakery and confectionary products, among others. Therefore, pectin recovery is of great importance. Scope and Approach The commercially available pectin is almost exclusively derived from citrus peels or apple pomace, by-products…
Fatty Acids and Triacylglycerols Profiles from Sicilian (Cold Pressed vs. Soxhlet) Grape Seed Oils
2021
Among the Sicilian economic productive sectors, that of wine production has today a considerable economic value. However, with the growth of this sector, notable was the increase in the production of waste, which to date is not only an economic damage for companies, but also a threat to the environment. It is known that waste from wine production has properties (e.g., antioxidants) which have potential reuse at cosmetic, pharmaceutical and nutritional levels to obtain economically sustainable applications. A new goal is given by the recovery of added value compounds from agri-food wastes and by-products. Grape seed oil is a promising vegetable fat and cold pressing does not involve the use …
Development of a qPCR assay for specific quantification of Botrytis cinerea on grapes
2010
The aim of this study was to develop a system for rapid and accurate real-time quantitative PCR (qPCR) identification and quantification of Botrytis cinerea , one of the major pathogens present on grapes. The intergenic spacer (IGS) region of the nuclear ribosomal DNA was used to specifically detect and quantify B. cinerea . A standard curve was established to quantify this fungus. The qPCR reaction was based on the simultaneous detection of a specific IGS sequence and also contained an internal amplification control to compensate for variations in DNA extraction and the various compounds from grapes that inhibit PCR. In these conditions, the assay had high efficiency (97%), and the limit o…
Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR
2011
Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to quantify fungal abundance in soils by incorporating, into a real-time Q-PCR using the SYBRGreen (R) method, a primer set already used to study the genetic structure of soil fungal communities. To satisfy the real-time Q-PCR requirements to enhance the accuracy and reproducibility of the detection technique, this study focused on the 18S rRNA gene conserved regions. These regions are little affec…