Search results for " Germplasm"
showing 10 items of 26 documents
Seed cryopreservation of Fraxinus angustifolia Vahl
2016
Fraxinus angustifolia is used for afforestation and for production of both valuable timber and manna , a substance with pharmacological applications. The aim of this research was to establish the optimized condition for cryopreservation of F. angustifolia seeds, in order to protect the germplasm of this species. Germination percentage and mean time of germination of non-treated seeds (control) and liquid nitrogen-treated seeds were established in water or in 10 -6 M gibberellic acid (GA 3 ). The seeds could be cryopreserved with 3% of moisture content (MC) and germinated easily in water (70.0 ± 5.0%), while seeds cryopreserved with 6% MC showed a physiological dormancy. This state could be …
Almond Germplasm in Bostanlyk Area (Uzbekistan): Preservation and Exploitation
2014
Almond germplasm has been studied throughout the world with high diversity identified in the Mediterranean Basin. Each production region is based on specific types of cultivars, frequently without a real commercial strategy. For this reason we still have a wide range of particularly interesting almond germplasm, especially for genetic improvement. In some parts of the world, local germplasm is still interesting for a restricted almond industry, usually related to a local or internal consumption. In this paper we provide details about the almond industry in the traditional area of Bostanlyk, Uzbekistan. Since 2007 a specific approach to evaluate the local germplasm has been applied; the most…
Assessment of genetic variation in SicilianHelichrysum(Asteraceae) and implication to germplasm conservation
2008
Some taxa of Sicilian Helichrysum related to sect. Stoeachadina have an uncertain systematic position; moreover, they are rare and endangered. In order to preserve these taxa in a genebank, a strategy to keep their genetic variation as high as possible is required. The high informative content of AFLP markers was used to assess genetic variations within and between populations and their relationships. The results show that all populations are genetically similar, even though there are two distinct groups: the first is the population referred to H. pendulum, and the second is represented by other populations belonging to several different specific and intraspecific taxa. The H. pendulum popu…
Study and characterization of polyphenol oxidase from eggplant (Solanum melongena L.)
2011
ABSTRACT: In this study the catecholase and cresolase activities of eggplant polyphenol oxidase (PPO) were investigated. Enzyme activity was determined by measuring the increase in absorbance using catechol as substrate and 3-methyl-2- benzothiazolinone hydrazone (MBTH) as coupled reagent. The effects of substrate specificity, heat inactivation, temperature, pH, and inhibitors were investigated to understand the enzymatic alteration of ready-to-eat preparations. Browning of vegetables was determined through a colorimeter. Decrease of lightness (L*) and increase of color difference values (ΔE*) were correlated with tissue browning. Antibrowning agents were tested on PPO under the same condit…
Molecular characterization of Sicilian lentil ecotypes using ISSR.
2013
APPROACHES TO STUDY BIODIVERSITY AND SALINITY TOLERANCE MECHANISMS IN MEDITERRANEAN FRUIT TREE SPECIES
Changes in global climate are going to increase the problem of drought and soil salinity in the Mediterranean area, where increases in temperature, drought and soil and water salinity are expected. Identification of plant genotypes with positive agronomic traits, such as salt tolerance, may reduce the effects of salinity and drought on productivity. Therefore, there is considerable effort being directed toward the development of salt-tolerant genotypes through plant breeding, with the aim to the introgression of salt-tolerance traits into new cultivars. The results presented in this thesis contributed to the develop of new tools to improve knowledge on the biodiversity and salinity toleranc…
EVALUATION OF SEED GERMINATION AND SEEDLING GROWTH OF SICILIAN AND INTERNATIONAL LOQUAT CULTIVARS
2015
Loquat (Eriobotrya japonica, Lindl) is commonly grafted on seedling rootstocks in Sicilian orchards. Seedling production is characterized by low germination percentage and velocity and slow plant growth. The aim of this study was to evaluate loquat seed germination of Sicilian and international cultivars. Seeds from 11 cultivars (Algerie, Bianco Dolce, BRT20, Claudia, Fiore, Golden Nugget, La Mantia, Magdal, Mercenò, Peluche, Tanaka) were collected from mature fruits in commercial orchards. Part of the seeds received a pretreatment with 1% HCl for 24 h. Germination data were collected at 30 days from sowing. Ninety days after germination, plants were transplanted into 2-L pots. The Sicilian…
INTRA-CULTIVAR DIVERSITY IN SOUTHERN ITALY OLIVE CULTIVARS DEPICTED BY MORPHOLOGICAL TRAITS AND SSR MARKERS
2014
In this study, SSR analysis was performed on a large number of putative clones of the main olive cultivars from two Italian regions, Sicily and Calabria, in order to study the genetic relatedness, and to detect any degree of genetic diversity, which can be used to unambiguously identify possible superior clones. These putative clones have been collected and observed in the last ten years, following an extensive investigation carried out in traditional olive growing areas, thanks to the reports of farmers, millers, technician, growers and nurseries. The set of microsatellite (SSR) markers used allowed the detection of the genetic diversity, resulting from somatic mutation, indicating the pre…
Intra-cultivar Diversity in sicilian and calabrian olive (olea europaea L.) Cultivars Depicted by Morphological Traits and SSR Markers
2012
In this study, SSR analysis was performed on a large number of putative clones of the main olive cultivars from two Italian regions, Sicily and Calabria, in order to study the genetic relatedness, and to detect any degree of genetic diversity, which can be used to unambiguously identify possible superior clones. These putative clones have been collected and observed in the last ten years, following an extensive investigation carried out in traditional olive growing areas, thanks to the reports of farmers, millers, technician, growers and nurseries. The set of microsatellite (SSR) markers used allowed the detection of the genetic diversity, resulting from somatic mutation, indicating the pre…