Search results for " Gl"

Human Papillomavirus Types 16, 18, and 31 Share Similar Endocytic Requirements for Entry

2013

ABSTRACT Human papillomavirus type 18 (HPV18), one of the HPVs with malignant potential, enters cells by an unknown endocytic mechanism. The key cellular requirements for HPV18 endocytosis were tested in comparison to those for HPV16 and -31 endocytoses. HPV18 (like HPV16 and -31) entry was independent of clathrin, caveolin, dynamin, and lipid rafts but required actin polymerization and tetraspanin CD151, and the viruses were routed to the same LAMP-1-positive compartment. Hence, the viruses shared similar cellular requirements for endocytic entry.

Colonization of adrenal glands and ovaries of mice by variants of HSV 1 and 2

1991

The herpes simplex virus (HSV)-infected mouse model was used to correlate histopathological lesions in adrenal glands and ovaries with the localisation of viral nucleic acids and viral antigens, employing in situ hybridization and immunohistochemistry. In the adrenals, the lesions were mainly restricted to the zona fasciculata and the zona reticularis, sometimes extending to the medulla. In the ovaries, lesions were detected in follicles and in the stroma. During the course of infection, HSV nucleic acids could be detected earlier than HSV proteins. Next to the center of necrotic foci mainly HSV proteins were detected, whereas peripheral cells were found to contain viral nucleic acids. In s…

Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16—Involvement of Tetraspanin-Enriched Microdomains (TEMs)

2008

BACKGROUND: Infectious entry of human papillomaviruses into their host cells is an important step in the viral life cycle. For cell binding these viruses use proteoglycans as initial attachment sites. Subsequent transfer to a secondary receptor molecule seems to be involved in virus uptake. Depending on the papillomavirus subtype, it has been reported that entry occurs by clathrin- or caveolin-mediated mechanisms. Regarding human papillomavirus type 16 (HPV16), the primary etiologic agent for development of cervical cancer, clathrin-mediated endocytosis was described as infectious entry pathway. METHODOLOGY/PRINCIPAL FINDINGS: Using immunofluorescence and infection studies we show in contra…

Tumor targeting of baculovirus displaying a lymphatic homing peptide.

2008

Background Tumor-associated cells and vasculature express attractive molecular markers for site-specific vector targeting. To attain tumor-selective tropism, we recently developed a baculovirus vector displaying the lymphatic homing peptide LyP-1, originally identified by ex vivo/in vivo screening of phage display libraries, on the viral envelope by fusion to the transmembrane anchor of vesicular stomatitis virus G-protein. Methods In the present study, we explored the specificity and kinetics of viral binding and internalization as well as in vivo tumor homing of the LyP-1 displaying virus to elucidate the applicability of baculovirus for targeted therapies. Results We demonstrated that th…

Ultrasound-guided viscosupplementation in glenohumeral microinstability in sports athletes

2014

(A,B) In vivo GCaMP6f signals recorded in layers M1, M5 and M9/10 of Mi1 (A) and Tm3 (B) neurons, before (blue, green) and after (gray, red) applicat…

2019

Sensory systems sequentially extract increasingly complex features. ON and OFF pathways, for example, encode increases or decreases of a stimulus from a common input. This ON/OFF pathway split is thought to occur at individual synaptic connections through a sign-inverting synapse in one of the pathways. Here, we show that ON selectivity is a multisynaptic process in the Drosophila visual system. A pharmacogenetics approach demonstrates that both glutamatergic inhibition through GluClα and GABAergic inhibition through Rdl mediate ON responses. Although neurons postsynaptic to the glutamatergic ON pathway input L1 lose all responses in GluClα mutants, they are resistant to a cell-type-specifi…

Vispārināto lineāro un vispārināto aditīvo modeļu salīdzinājums un pielietojumi

2019

Šajā darbā aprakstītas divas matemātiskās modelēšanas metodes, tās ir, vispārinātais lineārais modelis (generalized linear model (GLM)) un vispārinātais aditīvais modelis (generalized additive model (GAM)), un metodes, ar kurām tie ir saistīti. Darbā ir aprakstītas novērtēšanas metodes un kritēriji, kas tiek izmantoti šādu modeļu analizēšanai un novērtēšanai. Šī darba praktiskā daļa ir veikta programmā R, kur ir salīdzināti vispārinātais lineārais un vispārinātais aditīvais modelis ar mērķi izprast modeļu atšķirības un atšķirt gadījumus, kad labāk izvēlēties vienu vai otru modelēšanas metodi. Veicot darba praktisko daļu, tiek secināts, ka datiem, kuru izkliede nav vienkārša atkarībā no skai…

Motion perception in early glaucoma

2013

Purpose: The aim of our study was to underline the changes in the movement perception for early glaucoma. Our working hypothesis consisted in inquiring if the impairment of the magnocellular pathway may modify the movement perception capabilities in the visual field, more particularly in its peripheral area.Methods: We included 14 healthy subjects and 14 patients with early primary open angle glaucoma. A moving target was presented on a semicircular screen (1.8 m diameter); participants were asked to localize the Ending Point (EP) of each movement. Each stimulus consisted in a white dot (0,72° of diameter) moving horizontally with the imposed velocity profile. Two different laws of motion w…

Darbības glezniecības - radoša eksperimenta pielietošana vizuālās mākslas stundās

2018

Diplomdarba tēma ir “Darbības glezniecības – radošā eksperimenta pielietošana vizuālās mākslas stundās”. Diplomdarba mērķis ir izpētīt, vai vizuālās mākslas stundās 5. klasē var pielietot darbības glezniecību - radošo eksperimentu. Darbs sastāv no 3 daļām. Darba pirmajā daļā apskatīti darbības mākslas rašanās vēsturiskie apstākļi un attīstība, populārākie mākslinieki, viņu daiļrade, kā arī to mākslinieku darbība, kuru iedvesmas avots ir darbības glezniecība šodien. Otrajā daļā autors izstrādājis uzdevumu kopas izveides priekšnosacījumus. Trešajā daļā ir aprakstīta mācību uzdevumu kopas izveide, atspoguļota autora pedagoģiskā izmēģinājumdarbība, aprobēts izstrādātais mācību saturs un analizē…

Complex plant quality—microbiota–population interactions modulate the response of a specialist herbivore to the defence of its host plant

2022

Many specialist herbivores have evolved strategies to cope with plant defences, with gut microbiota potentially participating to such adaptations. In this study, we assessed whether the history of plant use (population origin) and microbiota may interact with plant defence adaptation. We tested whether microbiota enhance the performance of Melitaea cinxia larvae on their host plant, Plantago lanceolata and increase their ability to cope the defensive compounds, iridoid glycosides (IGs). The gut microbiota were significantly affected by both larval population origin and host plant IG level. Contrary to our prediction, impoverishing the microbiota with antibiotic treatment did not reduce larv…