Search results for " HSP70"

Adhesion and adaptive response of osteoblastlike cells (SAOS-2) grown on dfferent ti-6al-4v surfaces.

2004

Osteoblastic-like cells (SAOS-2) were seeded on three differently treated surfaces of Ti- 6Al-4V (T1: not treated; T2: chemically treated; T3: electrochemically treated). After 6h, the cells grown on T1 and T2 surfaces showed a typical poligonal morphology, they were strongly adherent to the surface and after 24h-48h they spread and formed a confluent monolayer. On the contrary, cells seeded on T3 showed heterogeneous morphology, decreased adhesion, loss of thin cellular expansions, and showed cellular shrinkage and apoptotic blebbing; only after 48h they were able to attach and form a monolayer after 4 days of seeding. Cell proliferation was significantly lower on T3 than T1 and T2 surface…

Adaptive response of osteoblasts grown on a titanium surface: morphology, cell proliferation and stress protein synthesis.

2005

Titanium is one of the most widely used biomaterials in orthopaedic, dental and trauma surgery. Superficial adhesion and cell proliferation represent the first steps of cell-biomaterial interactions. The efficacy of this early phase influences the subsequent ability to differentiate, and hence the knowledge of these cell activities is important for studying and improving the biocompatibility of biomaterials. The aim of this study was to test the adaptive ability of osteoblastic cells grown on titanium surfaces, including morphologic, proliferative and adaptative aspects.

HSP70 gene expression in Mytilus galloprovincialis hemocytes is triggered by moderate heat shock and Vibrio anguillarum, but not by V. splendidus or …

2005

Complete sequence of HSP70 cDNA from the mussel, Mytilus galloprovincialis was established before quantifying its expression following moderate heat shock or injection of heat-killed bacteria. HSP70 cDNA is comprised of 2378 bp including one ORF of 654 aa, with a predicted 70 bp 5'-UTR and a 343 bp 3'-UTR (GenBank, 18 Jan 05, AY861684). Alignment identity ranged from 89% for Crassostrea ariakensis to 72% for C. virginica. Curiously, HSP70 gene and cDNA sequences from M. galloprovincialis, deposited later (03 and 27 May), show only 73% identity with the present sequence. Meanwhile, characteristic motifs of the HSP70 family were located in conserved positions. Expression of HSP70 gene was qua…

Cellule staminali A6 di topo producono vescicole che contengono HSP70 e FGF-2

2006

Nickel, lead, and cadmium induce differential cellular responses in sea urchin embryos by activating the synthesis of different HSP70s.

2004

Treatment with heavy metals, such as nickel, lead or cadmium, elicits different cellular stress responses according to the metal used and the length of treatment. In Paracentrotus lividus embryos the inducible forms of HSP70 (HSP70/72) are different in molecular mass from the constitutively expressed HSP75, and they can be used as markers of cellular stress. Even a short treatment with each metal induces the synthesis of HSP70/72 which remain stable for at least 20 h and differ little in their isoelectric points. Continuous treatment from fertilization with nickel or lead produces late irregular pluteus embryos, with peak HSP70/72 synthesis at blastula followed by the arrest of synthesis by…

Induction of DNA damage and expression of heat shock protein HSP70 by polychlorinated biphenyls in the marine sponge Suberites domuncula Olivi

1999

The effects of different polychlorinated biphenyls (PCBs) including toxic coplanar non-ortho (PCB77) and non-planar mono-ortho (PCB118) and di-ortho (PCB153) congeners on the extent of DNA damage as well as on the expression of heat shock protein 70 (HSP70) were investigated in the marine sponge Suberites domuncula Olivi (Porifera; Demospongiae). A time-dependent increase in the number of DNA single-strand breaks, expressed as strand scission factor (SSF), was found after injection of a single dose of 25 mu g of PCB 118 or PCB 153 per gram wet mass of S. domuncula, using Fast Micromethod assay, which is based on the unwinding of DNA under alkaline conditions. The number of strand breaks ind…

Elevated cortisol modulates hsp70 and hsp90 gene expression and protein in sea bass head kidney and isolated leukocytes

2012

In fish, interactions between Hsps and cortisol are involved in stress modulated physiological processes including innate immune responses. Cortisol exerts a role in the regulation of Hsps synthesis. Fish head kidney is a lymphomieloid and endocrine organ releasing cortisol, and it is the central organ for immune-endocrine interactions. In sea bass, cortisol intraperitoneal injection and in vitro treatment of head kidney cells show that inducible Hsp70 and Hsp90 are modulated by this hormone. However, an inverse relationship between mRNA expression (real-time PCR) and Hsp70 and Hsp90 protein levels (densitometric band analysis) was found. Time-course assays indicate a cortisol-mediated regu…

Physical exercise induces rapid release of small extracellular vesicles into the circulation

2015

Cells secrete extracellular vesicles (EVs) by default and in response to diverse stimuli for the purpose of cell communication and tissue homeostasis. EVs are present in all body fluids including peripheral blood, and their appearance correlates with specific physiological and pathological conditions. Here, we show that physical activity is associated with the release of nano-sized EVs into the circulation. Healthy individuals were subjected to an incremental exercise protocol of cycling or running until exhaustion, and EVs were isolated from blood plasma samples taken before, immediately after and 90 min after exercise. Small EVs with the size of 100–130 nm, that carried proteins character…

Does Bacillus thuringiensis Affect the Stress and Immune Responses of Rhynchophorus ferrugineus Larvae, Females, and Males in the Same Way?

2022

Simple Summary Rhynchophorus ferrugineus is a destructive quarantine pest of palm trees, now widely distributed. Although broad-spectrum insecticides are often used to protect palm against R. ferrugineus, there is increasing concern about their effects on the environment and human health, especially where palm trees are located in urban areas. As an environmentally friendly entomopathogen, Bacillus thuringiensis (Bt) has been widely used to prevent other pest infestations. Although Bt products are the most sold bio-insecticides, there are still many interesting features to be investigated in the relationship of Bt and its hosts. We investigated the effect of Bt on larvae, females, and males…

Heat Shock Protein 70 Serum Levels Differ Significantly in Patients with Chronic Hepatitis, Liver Cirrhosis, and Hepatocellular Carcinoma

2014

Members of the heat shock protein 70 (HSP70) family play an important role in assisting protein folding, preventing protein aggregation and transport of proteins across membranes under physiological conditions. Following environmental (i.e., irradiation, chemotherapy), physiological (i.e., cell growth, differentiation), and pathophysiological (i.e., inflammation, tumorigenesis) stress, the synthesis of heat shock proteins (HSPs) is highly up-regulated, whereas protein synthesis in general is reduced. In contrast to normal cells, many tumor entities including hepatocellular carcinoma (HCC) overexpress HSP70, the major-stress-inducible member of the HSP70 family, present it on their cell surf…