Search results for " Interference"
showing 10 items of 340 documents
RNAi mediated acute depletion of Retinoblastoma protein (pRb) promotes aneuploidy in human primary cells via micronuclei formation
2009
BACKGROUND: Changes in chromosome number or structure as well as supernumerary centrosomes and multipolar mitoses are commonly observed in human tumors. Thus, centrosome amplification and mitotic checkpoint dysfunctions are believed possible causes of chromosomal instability. The Retinoblastoma tumor suppressor (RB) participates in the regulation of synchrony between DNA synthesis and centrosome duplication and it is involved in transcription regulation of some mitotic genes. Primary human fibroblasts were transfected transiently with short interfering RNA (siRNA) specific for human pRb to investigate the effects of pRb acute loss on chromosomal stability. RESULTS: Acutely pRb-depleted fibr…
SNAT2 silencing prevents the osmotic induction of transport system A and hinders cell recovery from hypertonic stress.
2005
AbstractUnder hypertonic conditions the induction of SLC38A2/SNAT2 leads to the stimulation of transport system A and to the increase in the cell content of amino acids. In hypertonically stressed human fibroblasts transfection with two siRNAs for SNAT2 suppressed the increase in SNAT2 mRNA and the stimulation of system A transport activity. Under the same condition, the expansion of the intracellular amino acid pool was significantly lowered and cell volume recovery markedly delayed. It is concluded that the up-regulation of SNAT2 is essential for the rapid restoration of cell volume after hypertonic stress.
Tribolium castaneum immune defense genes are differentially expressed in response to Bacillus thuringiensis toxins sharing common receptor molecules …
2015
In Tribolium castaneum larvae we have demonstrated by RNA interference knockdown that the Bacillus thuringiensis Cry3Ba toxin receptors Cadherin-like and Sodium solute symporter proteins are also functional receptors of the less active Cry3Aa toxin. Differences in susceptibility to B. thuringiensis infection might not only rely on toxin-receptor interaction but also on host defense mechanisms. We compared the expression of the immune related genes encoding Apolipophorin-III and two antimicrobial peptides, Defensin3 and Defensin2 after B. thuringiensis challenge. All three genes were up-regulated following Cry3Ba spore-crystal intoxication whereas only Defensins gene expression was induced u…
Structure Learning in Nested Effects Models
2007
Nested Effects Models (NEMs) are a class of graphical models introduced to analyze the results of gene perturbation screens. NEMs explore noisy subset relations between the high-dimensional outputs of phenotyping studies, e.g., the effects showing in gene expression profiles or as morphological features of the perturbed cell. In this paper we expand the statistical basis of NEMs in four directions. First, we derive a new formula for the likelihood function of a NEM, which generalizes previous results for binary data. Second, we prove model identifiability under mild assumptions. Third, we show that the new formulation of the likelihood allows efficiency in traversing model space. Fourth, we…
Integrated photonic routers driven by surface acoustic waves
2016
Durante la segunda mitad del siglo XX se produjo un desarrollo excepcional de la capacidad de transmitir y procesar información electrónicamente. Esto fue debido en parte a la capacidad de integrar en un único substrato una gran cantidad de transistores, que son las unidades fundamentales de procesamiento de datos. Este comportamiento quedó resumido en la ley de Moore, que predecía que el número de transistores integrados se duplicaría cada 24 meses. Esta tendencia, que se ha mantenido hasta la actualidad con transistores de tamaño nanométrico, no puede continuar indefinidamente. Uno de los problemas fundamentales reside en la capacidad limitada para disipar el calor que generan los disposi…
EMI Filter Re-Design in a SMPS with Inductor in Saturation
2021
This paper analyzes the design issues of the input EMI (Electro-Magnetic Interference) filter's in a switching mode power supply where the power inductor is operated in saturation. Starting with a SMPS equipped with linear inductor, the input filter is firstly designed to comply with Standards. Then, a new inductor with a smaller core size is employed in the same SMPS to exploit saturation. The EMI filter is re-designed, taking into account the increase of EMI introduced by the non-linear operation of the inductor. Finally, the reduction of the inductor's size and cost is compared with the increased size and cost of the EMI filter.
Diacylglycerol-containing oleic acid induces increases in [Ca(2+)](i) via TRPC3/6 channels in human T-cells.
2011
Though most of the studies have focused on the effects of free fatty acids on T-cell activation, fatty acids incorporated into plasma membrane phospholipids may also affect cell signaling via diacylglycerol (DAG), generally produced by phospholipid hydrolysis. In the present study, we have synthesized a DAG-containing oleic acid and studied its implication in the modulation of calcium signaling in human Jurkat T-cells. 1-palmitoyl-2-oleoyl-sn-glycerol (POG) induced a dose-dependent increase in [Ca(2+)](i). This effect was due to the presence of oleic acid at the sn-2 position as no differences were observed between POG and 1-stearoly-2-oleoyl-sn-glycerol (SOG). However, the substitution of …
COD and TPH analysis in slops experimental treatment plants: analytical problems.
2015
COD AND TPH ANALYSIS IN SLOPS EXPERIMENTAL TREATMENT PLANTS: ANALYTICAL PROBLEMS. In the last years, the persistence and accumulation of xenobiotic compounds in the environment created many disposal problems of oily wastewater generated by ships, mainly in engine-rooms (bilge waters) and by cleaning of tanker (slops). The high salinity levels (up to 25.000 mgL-1) and the pollutants concentration, both limit the chances of discharge into the sewer systems and address the disposal of these wastewaters to the sea. For these reasons it is necessary to treat such wastewater efficiently before discharging [1]. As a part of a broader project concerning slops treatments, this work addresses issues …
Exploiting programmable architectures for WiFi/ZigBee inter-technology cooperation
2014
The increasing complexity of wireless standards has shown that protocols cannot be designed once for all possible deployments, especially when unpredictable and mutating interference situations are present due to the coexistence of heterogeneous technologies. As such, flexibility and (re)programmability of wireless devices is crucial in the emerging scenarios of technology proliferation and unpredictable interference conditions. In this paper, we focus on the possibility to improve coexistence performance of WiFi and ZigBee networks by exploiting novel programmable architectures of wireless devices able to support run-time modifications of medium access operations. Differently from software…
An Improved Receiver Architecture for Cyclic-Prefixed OFDM
2009
A novel Orthogonal Frequency Division Multiplexing receiver architecture to be employed with standard (e.g. Wireless LAN) transmitters is proposed. It features enhanced error-rate performance with flexible computational complexity and robustness to imperfect channel estimation. It is based on exploitation of the redundancy available in the cyclic prefix after cancellation of interference from the previous block. In order to show the effectiveness of our proposal, a number of comparisons to the standard per-subcarrier receiver and a previously existing method are reported.