Search results for " LAB"

Anomalous alterations affecting microglia in the central nervous system of a fetus at 12 weeks of gestation: case report.

2003

We report here on the first documented case of profound alterations specifically affecting the microglial population within the nervous system during the fetal period. This case, derived at gestational week 12, was one amongst a series of second trimester brains currently being investigated with respect to microglial colonization of the human fetal brain. No significant pathological alterations could be identified upon gross macroscopy or following microscopic analysis of serial brain sections stained with cresyl fast violet (Nissl). By contrast, sections stained immunohistochemically to detect MHC class II (CR3/43) and CD68 (PG-M1) antigens revealed a marked pathological change in the morp…

Spatio-temporal expression of Prospero is finely tuned to allow the correct development and function of the nervous system in Drosophila melanogaster.

2007

0012-1606 (Print) Comparative Study Journal Article Research Support, Non-U.S. Gov't; Adaptive animal behaviors depend upon the precise development of the nervous system that underlies them. In Drosophila melanogaster, the pan-neural prospero gene (pros), is involved in various aspects of neurogenesis including cell cycle control, axonal outgrowth, neuronal and glial cell differentiation. As these results have been generally obtained with null pros mutants inducing embryonic lethality, the role of pros during later development remains poorly known. Using several pros-Voila (prosV) alleles, that induce multiple developmental and behavioral anomalies in the larva and in adult, we explored the…

A combined ex/in vivo assay to detect effects of exogenously added factors in neural stem cells.

2007

We describe a protocol developed/modified by our group for the ex vivo and in vivo assessment of the response to a soluble factor of murine neural stem cells from the adult sub-ventricular zone (SVZ). The procedure includes several experimental options that can be used either independently or in combination. Potential factor effects on self-renewal, survival and proliferation are assayed by means of neurosphere cultures, with the factor administered directly in vitro to the culture plates (Step 1) or infused in vivo immediately before tissue dissociation (Step 3). We also use bromodeoxiuridine (BrdU) retention to label slowly dividing cells in vivo and subsequently perform two different typ…

Supravital Uptake of Cationic Dyes by Mast Cell Granules: A Light and Electron Microscope Study

1994

Methylene blue and neutral red were selected for staining mast cell granules by supravital injections. A new technique was applied for embedding in paraffin and Araldite without dislocation or loss of dye. Stabilization and electron microscopic identification of the dyes were achieved by transforming them into electron-dense precipitates using phosphomolybdic acid dissolved in a paraformaldehyde-glutaraldehyde mixture to preserve the ultrastructure of the tissues. It was found that in general the intensity of the light microscopic staining correlated directly with the electron density. Closer study revealed that not all cytoplasmic granules exhibited the same strong affinity for the cationi…

The Measurement of Enzyme Activities in the Resting Human Polymorphonuclear Leukocyte — Critical Estimate of a Method

1993

As a system for study, the isolated human polymorphonuclear leukocyte combines the advantages of a quasi-non-invasive preparation with a nearly complete complement of enzymes of carbohydrate and energy metabolism. However, small sample volumes and, in some cases, very low enzyme activities make high demands on sample processing, storage, and performance of continuous measurements, if the enzyme activities are to be measured with acceptable reproducibility. In the presented study several aspects of homogenization, storage, and continuous measurement were scrutinized, to identify critical steps and consider ways of optimizing the method. Polymorphonuclear leukocytes were separated from the bl…

Metabolism of reduced pyridine nucleotides in ascites cell nuclei

1964

1. The conditions are described under which the fluorescence due to reduced pyridine nucleotides can be studied separately at nuclear and cytoplasmic sites of glass-grown ascites cells, by the use of a flow chamber in the microfluorimeter ofChance andLegallais.

Latvija. Pārskats par tautas attīstību 2017/2018: Sabiedriskā labuma radīšana un kolektīvo resursu nosargāšana Latvijā

2019

Meaning Extraction from Strappare Causatives in Italian

2021

The work targets a little-known causative construction of Italian whose causative verb is strappare ‘tear/extort/snatch’ (e.g. Ada strappò la confessione a Piero ‘Ada made Piero confess against his will’). In the active voice of this clause type, the subject, licensed by strappare, is invariably associated with the semantic role ‘Causer’ (as fare does in fare causatives, see [1]), whilst the post-verbal NP (e.g. confessione ‘confession’) is best analyzed as the predicate licensing the remaining syntactic function/s and the related semantic role/s. The NooJ grammar which the authors propose automatically extracts the meaning of strappare causatives by means of a novel type of semantic role. …

Morphological determination of the phototrophic community composition of biological soil crusts in coastal sand dunes in northern Germany

2022

This dataset comprises the microbial community composition of biological soil crusts in north-German sand dunes. For this we obtained enrichment cultures of phototrophic microorganisms, by placing fragments of biocrusts of the same Petri dishes as used for sequencing, in Petri dishes with Bold Basal (1N BBM) agarized medium (Bischoff and Bold 1963). Cultures were grown under standard laboratory conditions: with a 12-hour alteration of light and dark phases and irradiation of 25 μmol photons m-2 s-1 at a temperature 20 ± 5 ºС. Microscopic study of these raw cultures began in the third week of cultivation. Morphological examinations were performed using Olympus BX53 light microscope with Noma…

Shifting between cultural identities: The voice of preschool children

2018

 
 Aim. The purpose of this research is to capture the shift between two cultural identities, for a group of Romanian children enrolled in a German teaching preschool class, and to closely analyze the impact on cultural identity components.
 Methods. In this study 27 children, aged 3 to 6, were involved, together with their parents and two teachers. During one year of investigation, they were analyzed using participative observation, focus groups and in-depth interviews conducted in a public preschool.
 Results. The analysis reveals witch cultural identity components of this children undergo transformation, in what amount and with what impact considering the school environmen…