Search results for " LAB"

Serotoninergic innervation of nonprincipal cells in the cerebral cortex of the lizard Podarcis hispanica.

2004

The mechanism of serotoninergic transmission in the neo- and archicortex of mammals kis complex, including both synaptic and nonsynaptic components, direct actions on principal cells, and indirect effects mediated by GABAergic interneurons. Here we studied the termination pattern and synaptic organization of the serotoninergic afferents in the cerebral cortex of the lizard, Podarcis hispanica, which is considered to correspond in part to the mammalian hippocampal formation, with the aim of unraveling basic, phylogenetically preserved rules in the connectivity of this pathway. We demonstrate that serotoninergic afferents, visualized by immunostaining for serotonin itself, establish multiple …

Frequency of exposure of endangered Caspian seals to Canine distemper virus, Leptospira interrogans, and Toxoplasma gondii

2017

Canine distemper virus (CDV), Leptospira interrogans, and Toxoplasma gondii are potentially lethal pathogens associated with decline in marine mammal populations. The Caspian Sea is home for the endangered Caspian seal (Pusa caspica). In the late 1990s and early 2000s, CDV caused a series of mortality events involving at least several thousand Caspian seals. To assess current infection status in Caspian seals, we surveyed for antibodies to three pathogens with potential to cause mortality in marine mammals. During 2015-2017, we tested serum samples from 36, apparently healthy, Caspian seals, accidentally caught in fishing nets in the Caspian Sea off Northern Iran, for antibodies to CDV, L. …

Isolation and characterization of a fish F-type lectin from gilt head bream (Sparus aurata) serum.

2007

A novel fucose-binding lectin, designated SauFBP32, was purified by affinity chromatography on fucose-agarose, from the serum of the gilt head bream Sparus aurata. Electrophoretic mobility of the subunit revealed apparent molecular weights of 35 and 30 kDa under reducing and non-reducing conditions, respectively. Size exclusion analysis suggests that the native lectin is a monomer under the selected experimental conditions. Agglutinating activity towards rabbit erythrocytes was not significantly modified by addition of calcium or EDTA; activity was optimal at 37 degrees C, retained partial activity by treatment at 70 degrees C, and was fully inactivated at 90 degrees C. On western blot anal…

A serum fucolectin isolated and characterized from sea bass Dicentrarchus labrax

2001

A lectin specific for fucose and galactose was isolated by affinity chromatography on Sepharose CL-6B from the serum of Dicentrarchus labrax. The hemagglutinating activity against rabbit erythrocytes was calcium-independent, and reached its maximum at 37 degrees C. Two protein components were found in the hemagglutinating fractions eluted from the Sepharose column. Only the 34 kDa component (DLL2) eluted from the polyacrylamide gels (SDS-PAGE) showed agglutinating activity against rabbit erythrocytes. SDS-PAGE, in non-reducing conditions, revealed a single 66 kDa protein that reacted with antibodies to the 34 kDa component. Therefore, a dimeric structure stabilized by disulfide bonds can be…

El Trabajo Emocional desde una perspectiva clarificadora, tras treinta años de investigación

2014

El presente trabajo tiene como objetivo el análisis del concepto del Trabajo Emocional. Específicamente, a través de un enfoque integrador, se pretende clarificar a qué hace referencia el Trabajo Emocional y diferenciarlo de conceptos con los que comúnmente se relaciona en la literatura existente. Además, se analiza una de las últimas propuestas sobre cómo se podría producir el Trabajo Emocional aplicado al contexto de las organizaciones de servicios. Por último, se analizan los antecedentes que la literatura señala como aspectos potenciales para la aparición del trabajo emocional y las consecuencias que éste puede ocasionar en los resultados organizacionales, como es la calidad de servicio…

An Integrated Multi-service Software Simulation Platform: SIMPSONS Architecture

2005

This paper describes SIMPSONS (SIp and MPls Simulations On NS-2), which is a software platform for simulating multiple network scenarios, such as Telephony Over IP (TOIP), and multi-services networks extending the functionalities of Network Simulator NS-2. The innovative aspects of SIMPSONS is the complete integration of control protocols and traffic engineering mechanisms inside the same simulation tool. In fact, next generation networks must meet basically two fundamental requirements: support of Quality of Service (QoS) and Traffic Engineering (TE) functionalities. SIMPSONS is able to simulate DiffServ, MPLS, OSPF and SIP and their interaction in everything TOIP scenario. So with this po…

Wine label design as a strategic tool to attract consumers. A marketing study to know wine product positioning

2011

Yield and profitability of modified spanish bush and Y-trellis training systems for peach

2015

Peach trees trained to modified Spanish bush (MSB) and Y-trellis (Y) were evaluated and compared in the Mediterranean settings of Southern Italy. The observations included two peach (Rich May and Summer Rich) and two nectarine (Big Bang and Nectaross) cultivars. In the MSB system, trees were spaced at 4.5 x 2.5 m (888 trees/ha), whereas in the Y system, trees were spaced at 5.5 x 2 m (909 trees/ha). Costs at planting, yield per tree, fruit size grade, unit price of sold peaches for each size grade, amount and cost of materials and labor for cultural management, and grower's profit were quantified throughout all six years from planting and at full crop (year 4-6). Fixed costs of the MSB syst…

Dinamica del C labile in suoli forestali simulando l'impatto da incendio ed il successivo spegnimento con acqua marina

2009

Obiettivo del presente lavoro è stato quello di valutare, in diversi suoli forestali ed a condizioni controllate di laboratorio, l’impatto del riscaldamento da incendio e del successivo spegnimento con acqua marina sulla dinamica del C estraibile e sulla cinetica di respirazione. A tal fine, in un’area pedoclimatica omogenea, sono stati selezionati 4 suoli sotto diverse essenze forestali: Eucalyptus camaldulensis (EC), E. occidentalis (EO), Pinus halepensis (PH) e Cupressus sempervirens (CS). I campioni di suolo sono stati riscaldati a 25 (controllo), 160 e 380 °C per 30 minuti ed umidificati al 50% della capacità idrica di campo con acqua marina o distillata (controllo), ottenendo così 6 d…

A serum fucose-binding lectin (D1FBL) from adult Didentrarchus labrax is expressed in larva and juvenile tissue and contained in eggs

2010

The purification, cloning, sequencing, molecular properties and expression of a fucose-binding lectin from the serum of Dicentrarchus labrax (DlFBL) have been previously reported. We now describe the distribution and expression of DlFBL during fish ontogeny. Immunohistochemistry and in situ hybridization assays were carried out at various developmental stages (from 10 days posthatching larvae to juveniles). Another fucose-binding lectin, similar to DlFBL in biochemical, immunochemical and agglutinating properties, was extracted and purified from eggs and appeared to be localized in the embryo yolk sack residual. DlFBL was found in columnar and goblet cells of the intestinal epithelium of la…