Search results for " LAB"

Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode

2008

We describe a method to obtain the brightness and number of molecules at each pixel of an image stack obtained with a laser scanning microscope. The method is based on intensity fluctuations due to the diffusion of molecules in a pixel. For a detector operating in the analog mode, the variance must be proportional to the intensity. Once this constant has been calibrated, we use the ratio between the variance and the intensity to derive the particle brightness. Then, from the ratio of the intensity to the brightness we obtain the average number of particles in the pixel. We show that the method works with molecules in solution and that the results are comparable to those obtained with fluctu…

Multi-channel search for squarks and gluinos in root s=7 TeV pp collisions with the ATLAS detector at the LHC

2013

A search for supersymmetric particles in final states with zero, one, and two leptons, with and without jets identified as originating from b-quarks, in 4.7 fb[superscript −1] of √s = 7 TeV pp collisions produced by the Large Hadron Collider and recorded by the ATLAS detector is presented. The search uses a set of variables carrying information on the event kinematics transverse and parallel to the beam line that are sensitive to several topologies expected in supersymmetry. Mutually exclusive final states are defined, allowing a combination of all channels to increase the search sensitivity. No deviation from the Standard Model expectation is observed. Upper limits at 95 % confidence level…

In Vivo and In Vitro Binding of Vip3Aa to Spodoptera frugiperda Midgut and Characterization of Binding Sites by 125 I Radiolabeling

2014

ABSTRACT Bacillus thuringiensis vegetative insecticidal proteins (Vip3A) have been recently introduced in important crops as a strategy to delay the emerging resistance to the existing Cry toxins. The mode of action of Vip3A proteins has been studied in Spodoptera frugiperda with the aim of characterizing their binding to the insect midgut. Immunofluorescence histological localization of Vip3Aa in the midgut of intoxicated larvae showed that Vip3Aa bound to the brush border membrane along the entire apical surface. The presence of fluorescence in the cytoplasm of epithelial cells seems to suggest internalization of Vip3Aa or a fragment of it. Successful radiolabeling and optimization of the…

Regular versus as-needed budesonide and formoterol combination treatment for moderate asthma: A non-inferiority, randomised, double-blind clinical tr…

2015

Summary Background Treatment guidelines for patients with moderate persistent asthma recommend regular therapy with a combination of an inhaled corticosteroid and a longacting β 2 agonist plus as-needed rapid-acting bronchodilators. We investigated whether symptom-driven budesonide and formoterol combination therapy administered as needed would be as effective as regular treatment with this combination plus as-needed symptom-driven terbutaline for patients with moderate asthma. Methods In this non-inferiority randomised clinical trial, we recruited adult patients (18–65 years of age) with stable moderate persistent asthma, according to 2006 Global Initiative for Asthma guidelines. Patients …

202C.S.E. analgesia in active labor: effects of the intrathecal administration of l-Bupivacaine or Ropivacaine on onset and side effects

2006

Influencia del género sobre el proceso de desarrollo del síndrome de quemarse por el trabajo (Burnout) en profesionales de enfermería

2002

The purpose of this study was to analyze if gender differences establish significant differences in the levels and process of the burnout syndrome. Results indicate that the men (N = 72) perceive significantly more despersonalization that t he women (N = 258). In the sample of men p ersonal accomplishment was not a significant antecedent of despersonalization, while in the sample of women emotional exhaustion was not a significant antecedent of inclination to be absent. As a c onclusion it i s recommended that managers of personnel i n h ealth sector should take into consideration the gender differences in the intervention on the burnout syndrome.

Bērna labāko interešu principa saturs

2016

Bērna labāko interešu princips ir viens no svarīgākajiem starptautiski atzītajiem instrumentiem bērnu tiesību īstenošanā un attīstīšanā. Bērns kā tiesību subjekts ir ierobežots savu tiesību autonomā realizācijā, kā dēļ tas ir atkarīgs no citu personu motivācijas izvērtēt tā tiesības objektīvi. Šī iemesla dēļ, bērna labāko interešu principa klātbūtne ikvienā jautājumā, kas skar bērnu, ir izšķiroša. Bakalaura darbā “Bērna labāko interešu principa saturs” tiek atklāta bērna labāko interešu principa nozīme bērnu tiesību realizēšanā, tā ietekmes spēks un tvērums. Darbā tiek konstatēti arī galvenie problēmjautājumi pricipa praktiskajā piemērošanā un no tā izrietošās sekas. Bakalaura darbā tiek ap…

Bērnu un vecāku personiskās tiesības un pienākumi

2022

Bakalaura darba tēma ir bērnu un vecāku personiskās tiesības un pienākumi. Darba autore šo tēmu izvēlējās, jo bērnu un vecāku tiesības un pienākumi ir savstarpēji saistīti, un mijiedarbojoties veido savstarpējas attiecības, kas ir viens no svarīgākajiem faktoriem bērna attīstībā un izaugsmē. Bakalaura darba mērķis ir izpētīt bērnu un vecāku personisko tiesību un pienākumu regulējumu starptautiskajos tiesību aktos un šo tiesību un pienākumu tvērumu Latvijas Republikas normatīvajos aktos. Bakalaura darbā sniegts izklāsts un analizētas bērnu pamattiesības un pamatbrīvības, raksturots aizgādības tiesību jēdziens un tā sastāvdaļas. Bakalaura darbā padziļināti analizēti bērnu un vecāku saskarsmes…

Cancer cell targeting by CAR-T cells: A matter of stemness

2022

Chimeric antigen receptor (CAR)-T cell therapy represents one of the most innovative immunotherapy approaches. The encouraging results achieved by CAR-T cell therapy in hematological disorders paved the way for the employment of CAR engineered T cells in different types of solid tumors. This adoptive cell therapy represents a selective and efficacious approach to eradicate tumors through the recognition of tumor-associated antigens (TAAs). Binding of engineered CAR-T cells to TAAs provokes the release of several cytokines, granzyme, and perforin that ultimately lead to cancer cells elimination and patient’s immune system boosting. Within the tumor mass a subpopulation of cancer cells, known…

Measurement of T Cell Activation After 16‐hr In Vitro Stimulation with Concanavalin A

2010

A flow cytometry assay that can be used to directly determine the proportion of activated T lymphocytes in human whole blood samples after stimulation with concanavalin A is presented here. Human whole blood is incubated with fluorescently labeled antibodies (against CD3, CD4, CD8, and CD69), erythrocytes are then lysed, and the samples are analyzed using a flow cytometer. The assay presented is able to differentiate between CD4+ and CD8+ T lymphocytes. Thus, it is possible to quantify both lymphocyte populations in parallel, as well as the respective proportions of activated T lymphocytes, all from one sample. An additional advantage of this assay is that it was developed to assay whole bl…