Search results for " LAB"

showing 10 items of 2393 documents

Immune characterization of the HBHA-specific response in Mycobacterium tuberculosis-infected patients with or without HIV infection.

2017

Introduction RD1-based Interferon-γ Release Assays (IGRAs) cannot distinguish latent from active tuberculosis (TB) disease. Conversely, a positive response to heparin-binding haemagglutinin (HBHA)-based IGRAs, among TB-infected subjects, correlates with Mycobacterium tuberculosis (Mtb) containment and low risk of TB progression. The aim of this study was to characterize HBHA-immune responses in HIV-infected and uninfected subjects with active TB or latent TB infection (LTBI). Methods 49 subjects were prospectively enrolled: 22 HIV-uninfected (13 TB, 9 LTBI) and 27 HIV-infected (12 HIV-TB, 15 HIV-LTBI). Whole blood and peripheral blood mononuclear cells were stimulated with HBHA and RD1 anti…

Genetics and Molecular Biology (all)0301 basic medicineBacterial DiseasesRNA virusesCD4-Positive T-LymphocytesMalePhysiologylcsh:MedicineHIV InfectionsCD8-Positive T-LymphocytesPathology and Laboratory MedicineBiochemistryMemory T cellsWhite Blood CellsImmunodeficiency VirusesInterferonAnimal CellsImmune PhysiologyLectinsMedicine and Health SciencesProspective Studieslcsh:ScienceInnate Immune SystemMultidisciplinarybiologyT CellsInterleukinvirus diseasesMiddle AgedFlow Cytometry3. Good healthActinobacteriaInfectious DiseasesMedical MicrobiologyViral PathogensVirusesDisease ProgressionCytokinesFemaleCellular TypesPathogensmedicine.drugResearch ArticleAdultTuberculosisImmune CellsImmunologyCytotoxic T cellsEnzyme-Linked Immunosorbent AssayPeripheral blood mononuclear cellMicrobiologySettore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICAMycobacterium tuberculosis03 medical and health sciencesInterferon-gammaImmune systemAntigenRetrovirusesmedicineTuberculosisHumansMicrobial PathogensAdult; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cytokines; Disease Progression; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; HIV Infections; Humans; Interferon-gamma; Lectins; Male; Middle Aged; Prospective Studies; Tuberculosis; Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)Blood CellsBacteriaLentiviruslcsh:ROrganismsBiology and Life SciencesProteinsHIVCell BiologyMolecular Developmentbiology.organism_classificationmedicine.diseaseTropical DiseasesVirology030104 developmental biologyAgricultural and Biological Sciences (all)Immune SystemImmunologylcsh:QInterferonsCD8Mycobacterium TuberculosisDevelopmental BiologyPLoS ONE
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Sex difference in the interaction of alcohol intake, hepatitis B virus, and hepatitis C virus on the risk of cirrhosis

2017

Background The joint effect of the interaction of alcohol intake, hepatitis B virus (HBV) and hepatitis C virus (HCV) on the risk of cirrhosis is still unexplored because a large sample size is required for this investigation. Objective Evaluation of interaction of HBV, HCV and alcohol abuse on the risk of cirrhosis. Design We analysed 12,262 consecutive patients with chronic liver disease of various aetiologies referring to 95 Italian liver units in 2001 or 2014. To evaluate the interaction between alcohol abuse, HBV infection, and HCV infection, patients unexposed to either factors were used as reference category. Adjustment for BMI and age was done by multiple logistic regression analysi…

Genetics and Molecular Biology (all)0301 basic medicineLiver CirrhosisRNA virusesMaleChronic HepatitisCirrhosislcsh:MedicineAlcohol abuseHepacivirusSex FactorChronic liver diseasemedicine.disease_causeBiochemistryGastroenterologyChronic Liver Disease0302 clinical medicineRisk FactorsMedicine and Health Scienceslcsh:SciencePathology and laboratory medicineMultidisciplinaryAlcohol ConsumptionHepatitis C virusLiver DiseasesFatty livervirus diseasesHepatitis CHepatitis BMedical microbiologyMiddle AgedHepatitis BHepatitis CCirrhosisOncologyVirusesCoinfection030211 gastroenterology & hepatologyFemalePathogensResearch ArticleHumanAdultmedicine.medical_specialtyHepatitis B virusAlcohol DrinkingLiver CirrhosiGastroenterology and HepatologyMicrobiologyCarcinomas03 medical and health sciencesSex FactorsInternal medicineGastrointestinal TumorsmedicineHumansNutritionAgedHepatitis B virusBiochemistry Genetics and Molecular Biology (all)Flavivirusesbusiness.industryRisk Factorlcsh:ROrganismsViral pathogensBiology and Life SciencesCancers and NeoplasmsHepatocellular Carcinomamedicine.diseaseVirologydigestive system diseasesHepatitis virusesAdult; Aged; Alcohol Drinking; Female; Hepatitis B; Hepatitis C; Humans; Liver Cirrhosis; Male; Middle Aged; Risk Factors; Sex Factors; Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)DietMicrobial pathogensFatty Liver030104 developmental biologyAgricultural and Biological Sciences (all)lcsh:Qbusiness
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Impact of Safety-Related Dose Reductions or Discontinuations on Sustained Virologic Response in HCV-Infected Patients: Results from the GUARD-C Cohor…

2015

Background: Despite the introduction of direct-acting antiviral agents for chronic hepatitis C virus (HCV) infection, peginterferon alfa/ribavirin remains relevant in many resource-constrained settings. The non-randomized GUARD-C cohort investigated baseline predictors of safety-related dose reductions or discontinuations (sr-RD) and their impact on sustained virologic response (SVR) in patients receiving peginterferon alfa/ribavirin in routine practice. Methods: A total of 3181 HCV-mono-infected treatment-naive patients were assigned to 24 or 48 weeks of peginterferon alfa/ribavirin by their physician. Patients were categorized by time-to-first sr-RD (Week 4/12). Detailed analyses of the i…

Genetics and Molecular Biology (all)MaleChronic HepatitisHepacivirusRibavirin/adverse effectsAsthenia/chemically inducedHepacivirusPolyethylene GlycolBiochemistryPolyethylene GlycolsBody Mass IndexChronic Liver Disease0302 clinical medicineNeutropenia/chemically inducedInterferon-alpha/adverse effectsMedicineChroniclcsh:ScienceLiver Diseasesvirus diseasesAntiviral Agents/adverse effectsCohortScience & Technology - Other Topics030211 gastroenterology & hepatologyDrug Therapy CombinationCohort studyHumanmedicine.medical_specialtyAlpha interferonGastroenterology and HepatologyAntiviral AgentsMicrobiologyDose-Response Relationship03 medical and health sciencesPharmacotherapyHepatitis C Chronic/drug therapyDose Prediction MethodsDrug TherapyAnemia/chemically inducedHumansHemoglobinAgedMedicine and health sciencesBiochemistry Genetics and Molecular Biology (all)HepaciviruScience & TechnologyDose-Response Relationship DrugFlaviviruseslcsh:ROrganismsBiology and Life SciencesProteinsmedicine.diseasedigestive system diseaseschemistryAgricultural and Biological Sciences (all)Withholding TreatmentAstheniaImmunologyProportional Hazards Modellcsh:QHuman medicineRNA virusesPhysiologylcsh:MedicinePeginterferon-alfaPolyethylene Glycols/adverse effectsAdult; Aged; Anemia; Antiviral Agents; Asthenia; Cohort Studies; Dose-Response Relationship Drug; Drug Therapy Combination; Female; Hepacivirus; Hepatitis C Chronic; Host-Pathogen Interactions; Humans; Interferon-alpha; Male; Middle Aged; Neutropenia; Outcome Assessment (Health Care); Polyethylene Glycols; Proportional Hazards Models; RNA Viral; Recombinant Proteins; Ribavirin; Withholding Treatment; Agricultural and Biological Sciences (all); Biochemistry Genetics and Molecular Biology (all); Medicine (all)Cohort Studieschemistry.chemical_compoundOutcome Assessment Health CareMedicine and Health Sciences030212 general & internal medicineViralPathology and laboratory medicineMultidisciplinarybiologyHepatitis C virusPharmaceuticsMedicine (all)AnemiaHepatitis CHematologyRecombinant ProteinOutcome Assessment (Health Care)/methodsMiddle AgedMedical microbiologyHepatitis CRecombinant ProteinsHost-Pathogen InteractionMultidisciplinary SciencesPhysiological ParametersResearch DesignCombinationHost-Pathogen InteractionsVirusesRNA ViralFemaleDrugPathogensHost-Pathogen Interactions/drug effectsResearch ArticleAdultNeutropeniaClinical Research DesignResearch and Analysis MethodsOutcome Assessment (Health Care)Internal medicineRibavirinRecombinant Proteins/adverse effectsRNA Viral/bloodAdult; Aged; Anemia; Antiviral Agents; Asthenia; Cohort Studies; Dose-Response Relationship Drug; Drug Therapy Combination; Female; Hepacivirus; Hepatitis C Chronic; Host-Pathogen Interactions; Humans; Interferon-alpha; Male; Middle Aged; Neutropenia; Outcome Assessment (Health Care); Polyethylene Glycols; Proportional Hazards Models; RNA Viral; Recombinant Proteins; Ribavirin; Withholding TreatmentAdult; Aged; Anemia; Antiviral Agents; Asthenia; Cohort Studies; Dose-Response Relationship Drug; Drug Therapy Combination; Female; Hepacivirus; Hepatitis C Chronic; Host-Pathogen Interactions; Humans; Interferon-alpha; Male; Middle Aged; Neutropenia; Outcome Assessment (Health Care); Polyethylene Glycols; Proportional Hazards Models; RNA Viral; Recombinant Proteins; Ribavirin; Withholding Treatment; Medicine (all); Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)Proportional Hazards ModelsAntiviral Agentbusiness.industryRibavirinBody WeightHepacivirus/drug effectsViral pathogensInterferon-alphaHepatitis C Chronicbiology.organism_classificationHepatitis virusesMicrobial pathogensRNAAdverse EventsCohort StudiebusinessPloS one
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Mediterranean diet adherence and synergy with acute myocardial infarction and its determinants: A multicenter case-control study in Italy

2018

Background Cardiovascular diseases are the leading causes of mortality and morbidity in Western countries. The possible synergistic effect of poor adherence to a Mediterranean diet (MD) and other risk factors for acute myocardial infarction (AMI) such as hypertension, cholesterol, ever smoker, BMI> 25, diabetes, has not been deeply studied. Design Case-control study. Methods Patients with first AMI and controls from four tertiary referral Italian centers were screened for enrolment. Dietary information was collected through a questionnaire and a MD adherence score was calculated. Physical activity and smoking habits were also registered. The Synergy Index was calculated according to Rothman…

Genetics and Molecular Biology (all)MaleMultivariate analysisMediterranean dietTertiary Care CenterMyocardial Infarctionlcsh:MedicineBlood PressureMediterranean030204 cardiovascular system & hematologyPathology and Laboratory MedicineDiet MediterraneanVascular MedicineBiochemistryGeographical locationsTertiary Care CentersHabitschemistry.chemical_compoundEndocrinology0302 clinical medicineRisk FactorsSurveys and QuestionnairesMedicine and Health SciencesSmoking HabitsSurveys and Questionnaire030212 general & internal medicineMyocardial infarctionlcsh:ScienceMultidisciplinarySmokingMiddle AgedEuropeHyperlipidemiaItalyResearch DesignSettore MED/42HypertensionFemaleCase-Control StudieHumanResearch Articlemedicine.medical_specialtyAged; Case-Control Studies; Feeding Behavior; Female; Humans; Hypertension; Italy; Male; Middle Aged; Myocardial Infarction; Risk Factors; Smoking; Surveys and Questionnaires; Tertiary Care Centers; Diet Mediterranean; Patient ComplianceReferralEndocrine DisordersSmoking habitHypercholesterolemiaCardiologyResearch and Analysis Methods03 medical and health sciencesSigns and SymptomsDiagnostic MedicineDiabetes mellitusInternal medicineDiabetes MellitusAged; Case-Control Studies; Feeding Behavior; Female; Humans; Hypertension; Italy; Male; Middle Aged; Myocardial Infarction; Risk Factors; Smoking; Surveys and Questionnaires; Tertiary Care Centers; Diet Mediterranean; Patient Compliance; Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)medicineHumansEuropean UnionNutritionAgedBehaviorCholesterolbusiness.industryRisk Factorlcsh:RCase-control studyBiology and Life SciencesFeeding Behaviormedicine.diseaseDietAgricultural and Biological Sciences (all)chemistryMetabolic DisordersCase-Control StudiesPatient Compliancelcsh:QPeople and placesbusinessPLOS ONE
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Factors influencing psychological well-being in patients with Parkinson's disease.

2017

Background Both motor and non-motor symptoms could contribute to significant deterioration of psychological well-being in patients with Parkinson's disease (PD). However, its assessment has been only indirectly evaluated using tools based on health-related quality of life (HRQoL), such as the PDQ-39 scale. Objectives To evaluate psychological well-being in PD using a specific tool of assessment, the Psychological Well-being Scale (PWS), and its clinical correlates. Methods This article reports data of patients' perception of health state, as measured by means of the PWS, from an epidemiological, cross-sectional study conducted in Italian PD patients (FORTE Study). We tested possible relatio…

Genetics and Molecular Biology (all)MaleParkinson's diseaseEmotionsPoison controlSocial Scienceslcsh:MedicinePathology and Laboratory MedicineBiochemistrySeverity of Illness IndexAged; Depression; Fatigue; Female; Humans; Italy; Male; Middle Aged; Parkinson Disease; Psychiatric Status Rating Scales; Quality of Life; Severity of Illness Index; Surveys and Questionnaires; Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)0302 clinical medicineMathematical and Statistical TechniquesQuality of lifeSurveys and QuestionnairesMedicine and Health SciencesMedicinePsychologySurveys and Questionnaire030212 general & internal medicinelcsh:ScienceDepression (differential diagnoses)FatigueMultidisciplinaryMovement DisordersDepressionNeurodegenerative DiseasesParkinson DiseaseMiddle AgedPsychiatric Status Rating ScalehumanitiesNeurologyItalyPhysical SciencesFemaleStatistics (Mathematics)Clinical psychologyResearch ArticleHumanResearch and Analysis Methods03 medical and health sciencesSigns and SymptomsDiagnostic MedicineSeverity of illnessInjury preventionMental Health and PsychiatryHumansStatistical MethodsAgedPsychiatric Status Rating Scalesparkinson's disease quality of lifeAnalysis of VarianceBiochemistry Genetics and Molecular Biology (all)business.industryMood Disorderslcsh:RBeck Depression InventoryBiology and Life Sciencesmedicine.diseaseHealth CareAgricultural and Biological Sciences (all)Psychological well-beingAged; Depression; Fatigue; Female; Humans; Italy; Male; Middle Aged; Parkinson Disease; Psychiatric Status Rating Scales; Quality of Life; Severity of Illness Index; Surveys and QuestionnairesQuality of Lifelcsh:QbusinessSleep Disorders030217 neurology & neurosurgeryMathematicsPLoS ONE
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In silico identification and experimental validation of hits active against KPC-2 β-lactamase

2018

Bacterial resistance has become a worldwide concern, particularly after the emergence of resistant strains overproducing carbapenemases. Among these, the KPC-2 carbapenemase represents a significant clinical challenge, being characterized by a broad substrate spectrum that includes aminothiazoleoxime and cephalosporins such as cefotaxime. Moreover, strains harboring KPC-type β-lactamases are often reported as resistant to available β-lactamase inhibitors (clavulanic acid, tazobactam and sulbactam). Therefore, the identification of novel non β-lactam KPC-2 inhibitors is strongly necessary to maintain treatment options. This study explored novel, non-covalent inhibitors active against KPC-2, …

Genetics and Molecular Biology (all)Proteomics0301 basic medicineCefotaximeKlebsiella pneumoniaePathology and Laboratory MedicinePhysical ChemistryBiochemistryKlebsiella PneumoniaeDatabase and Informatics MethodsBiochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)AntibioticsKlebsiellaCatalytic DomainMedicine and Health Sciencespolycyclic compoundsDrug InteractionsCrystallographyMultidisciplinarybiologyAntimicrobialsOrganic CompoundsProteomic DatabasesChemistryPhysicsQRDrugsSulbactamCondensed Matter PhysicsBacterial PathogensChemistryBiochemistryMedical MicrobiologyPhysical SciencesCrystal StructureMedicinePathogensbeta-Lactamase InhibitorsResearch Articlemedicine.drugScienceIn silico030106 microbiologySulfonamideResearch and Analysis MethodsMicrobiologyMeropenemTazobactambeta-Lactamases03 medical and health sciencesBacterial ProteinsMicrobial ControlClavulanic acidmedicineSolid State PhysicsMicrobial PathogensPharmacologyLigand efficiencyChemical BondingBacteriaOrganic ChemistryChemical CompoundsOrganismsBiology and Life SciencesHydrogen Bondingbiochemical phenomena metabolism and nutritionbiology.organism_classificationbacterial infections and mycosesAmidesBiological Databases030104 developmental biologyAgricultural and Biological Sciences (all)
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Scanning electron microscopy of heterochromatin in chromosome spreads of male germ cells in Schistocerca gregaria (Acrididae, Orthoptera) after tryps…

1996

Chromosome spreads, prepared from testes of the desert locust Schistocerca gregaria, were analyzed using scanning electron microscopy (SEM) after varying periods of preincubation in trypsin. The emphasis of the study was on the appearance of heterochromatin. A trypsin pretreatment of 5 sec resulted in a smooth surface on the chromatin throughout and the heterochromatin was highly electron-emissive. The facultatively heterochromatic X chromosome was clearly visible in interphase spermatogonia and in pachytene and late prophase I spermatocytes. Chromomeres of autosomal bivalents could be recognized in pachytene cells. Centromeric heterochromatin segments were very prominent in autosomes of la…

GeneticsMaleHistologyAutosomeEuchromatinHeterochromatinChromosomeGeneral MedicineGrasshoppersBiologySpermatozoaChromosomesCell biologyTrypsinizationMedical Laboratory TechnologyMicroscopy ElectronMeiosisHeterochromatinMicroscopy Electron ScanningAnimalsTrypsinMitosisX chromosomeBiotechnichistochemistry : official publication of the Biological Stain Commission
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Commuting and local labour market areas in the Region of Valencia, Spain, 1991-2001

2007

En este trabajo se analiza la evolución de la movilidad residencia-trabajo en la Comunidad Valenciana entre 1991 y 2001 y se delimitan para ambos años los mercados locales de trabajo (MLT) valencianos mediante una variación del procedimiento empleado en el Reino Unido para la definición de las denominadas Travel-to-Work Areas. Esta regionalización permite superar el concepto administrativo de municipio/ciudad e identificar áreas funcionales coherentes con los nuevos modelos urbanos derivados del aumento generalizado de la movilidad de la población y el surgimiento de la ‘ciudad difusa’. This article deals with the evolution of commuting in the Region of Valencia, Spain, between 1991 and 200…

Geography (General)Local labour market areasCommutingCommuting ; Local labour market areas ; Functional areas ; Region of ValenciaÁreas funcionalesMovilidad residencia-trabajo (commuting) ; Mercados locales de trabajo ; Áreas funcionales ; Comunidad ValencianaMercados locales de trabajoEconomía AplicadaRegion of ValenciaFunctional areasValencia (Comunidad Autónoma)Environmental sciences:GEOGRAFÍA [UNESCO]:GEOGRAFÍA::Geografía humana ::Geografía social [UNESCO]Movilidad residencia-trabajo (commuting)G1-922GE1-350UNESCO::GEOGRAFÍA::Geografía humana ::Geografía socialComunidad ValencianaUNESCO::GEOGRAFÍA
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Ring test for whole-sediment toxicity assay with -a- benthic marine diatom

2009

7 páginas, 3 figuras, 3 tablas.

Geologic SedimentsEnvironmental EngineeringTest (biology)AlgaeAquatic plantToxicity TestsWhole-sediment toxicity assayEnvironmental ChemistryEcotoxicologyCylindrotheca closteriumRing testWaste Management and DisposalDiatomsPortugalbiologyClinical Laboratory TechniquesEcologyMarine diatomZ-scorefungiReproducibility of ResultsSedimentbiology.organism_classificationPollutionClosteriumSpainBenthic zoneEnvironmental chemistryToxicityBiological AssayWater Pollutants ChemicalEnvironmental MonitoringScience of The Total Environment
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Corrigendum to “Immune parameters identify Italian centenarians with a longer five-year survival independent of their health and functional status” […

2016

8 a Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy 9 b CIG—Interdepartmental Centre L. Galvani, University of Bologna, Bologna, Italy 10 c Physics and Astronomy Department, University of Bologna, Bologna, Italy 11 d Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy 12 e Immunosenescence Unit, Department of Pathobiology and Biomedical Methodologies, University of Palermo, Palermo, Italy 13 f Geriatric Unit IRCCS Ca' Granda Foundation Maggiore Policlinico Hospital, University of Milan, Italy 14 g Department of Clinical Sciences and Community Health, University of Milan, Italy 15 h Experimental Laboratory o…

GerontologyAgingmedicine.medical_specialtybusiness.industryCell BiologyExperimental laboratoryBiochemistryEndocrinologyFamily medicineGeneticsmedicineFunctional statusbusinessMolecular BiologyExperimental Gerontology
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