Search results for " LAB"
showing 10 items of 2393 documents
Highly efficient transport of carboxyfluorescein diacetate succinimidyl ester into COS7 cells using human papillomavirus-like particles
2003
AbstractHuman papillomavirus virus-like particles (VLPs) have recently been used to deliver genes into mammalian cells in vitro and in vivo. Here, we investigated whether VLPs may serve as an efficient carrier of low molecular weight compounds (e.g. hormones, vitamins, peptides etc.) into cells. COS7 cells were incubated with recombinant HPV-16L1/L2 VLPs labelled with the fluorescence dye carboxyfluorescein diacetate succinimidyl ester. Using flow cytometry, we demonstrate that labelled VLPs can specifically bind to the cell surface followed by their complete internalisation. Our results indicate that VLPs are promising vehicles for highly efficient delivery of low molecular weight compound…
Facultat de Ciències Socials
2008
La Universitat de València t'obri les seues portes. En les seues Facultats i Escoles Universitàries descobriràs una àmplia oferta d'estudis, equipament docent d'última tecnologia, instal·lacions esportives i culturals i una completa xarxa de biblioteques. Tot el que necessites per a accedir a una formació integral que garantisca el teu futur professional.
Der Fr�hnachweis des Herzinfarktes durch Bestimmung der Wasserstoffionenkonzentration im Herzmuskel mit Indicatorpapier
1965
Es wird eine einfache Methode zum Fruhnachweis des Herzinfarktes beschrieben. Durch Messung der Wasserstoffionenkonzentration an Kryostatschnitten vom Herzen mit pH-Indicatorpapier wird ein bei Ratten und Katzen erzeugter Ischamiebezirk bereits 5 min nach Unterbindung einer Coronararterie im Herzmuskel nachweisbar. Die in der Literatur beschriebene Anderung der Wasserstoffionenkonzentration in einer Nekrose wird fur den Herzinfarkt bestatigt und dient zu seinem Nachweis bis zum Auftreten von lichtmikroskopisch erkennbaren Nekrosezeichen.
Plasma Insulin Levels and Hypoglycemia Affect Subcutaneous Interstitial Glucose Concentration
2018
[EN] Background: Continuous glucose monitoring (CGM) accuracy during hypoglycemia is suboptimal. This might be partly explained by insulin or hypoglycemia-induced changes in the plasma interstitial subcutaneous (SC) fluid glucose gradient. The aim of the present study was to assess the role of plasma insulin (PI) and hypoglycemia itself in the plasma and interstitial SC fluid glucose concentration in patients with type 1 diabetes mellitus. Methods: Eleven subjects with type 1 diabetes (age 36.59.1 years, HbA(1c) 7.90.4% [62.8 +/- 2.02mmol/mol]; mean +/- standard deviation) were evaluated under hyperinsulinemic euglycemia and hypoglycemia. Each subject underwent two randomized crossover clam…
Costruzione e validazione del questionario DIDLab “La didattica Laboratoriale: apprendimenti e stili di conduzione con l’approccio IBSE”
2021
Il presente lavoro espone gli esiti della costruzione e validazione del questiona-rio “La didattica Laboratoriale: apprendimenti e stili di conduzione con l’ap-proccio IBSE” finalizzato a misurare il grado di conoscenza dell’approccio In-quiry-Based Science Education e lo stile di conduzione nella didattica labora-toriale, che ha visto coinvolti 523 corsisti del corso di specializzazione per le attività di sostegno didattico dell’Università degli Studi di Palermo; il test di misurazione costruito è composto da 42 item suddiviso in cinque macro-aree su scala Likert a 5 punti. Dall’analisi quantitativa condotta è emerso che il questionario ha buona con-cordanza, pensato per indagare sulle con…
Inquiry based Laboratory. Una ricerca con i futuri docenti di sostegno.
2021
La formazione degli insegnanti in laboratorio è particolarmente rilevante perché consente di a t t i v a r e p r o c e s s i d i i n s e g n a m e n t o / apprendimento in cui gli alunni diventano protagonisti dei propri percorsi. Il laboratorio rappresenta la modalità trasversale che caratterizza la didattica e la formazione dei futuri docenti. Attraverso la didattica laboratoriale gli studenti hanno la possibilità di confrontarsi, di mettere in atto le abilità critiche, logiche e creative per esplorare la realtà circostante. Ciò si coniuga perfettamente con le possibilità che garantisce un laboratorio scientifico Inquiry-Based Learning centrato sul processo di investigazione. Nel presente…
Il diritto di difesa degli staff member nei sistemi di giustizia interna dell'Organizzazione Internazionale del Lavoro, dell'Organizzazione delle Naz…
Trayectorias laborales de autóctonos e inmigrantes en España: entre la convergencia y la segmentación. Un análisis de la Muestra Continua de Vida Lab…
2011
Este trabajo es un análisis de las trayectorias laborales de trabajadores autóctonos y de origen extranjero a partir de la muestra continua de vidas laborales de la seguridad social (en adelante MCVL). Se exploraron dos hipótesis. Por un lado, la antigüedad en el mercado de trabajo y la cualificación producen con el tiempo una “asimilación ocupacional de la inmigración” con los trabajadores autóctonos; por otro, la inserción de la inmigración en determinados nichos del mercado laboral limita la convergencia con los trabajadores autóctonos. Los resultados señalan que la asimilación se produce de forma estratificada en algunas pocas categorías profesionales, mientras persiste la brecha de acu…
Technical and health governance aspects of the External Quality Assessment Scheme for the SARS-CoV-2 molecular tests: institutional experience perfor…
2022
Abstract Objectives Since December 2019, the worldwide public health has been threatened by a severe acute respiratory syndrome caused by Coronavirus-2. From the beginning, a turning point has been the identification of new cases of infection, in order to minimize the virus spreading among the population. For this reason, it was necessary introducing a panel of tests able to identify positive cases, which became crucial for all countries. Methods As a Regional Reference Centre, the CRQ Laboratory (Regional Laboratory for the Quality Control) developed and conducted an External Quality Assessment (EQA) panel of assay, so as to evaluate the quality of real-time reverse transcription polymeras…
Oxygen consumption of F0 and F1 larval and juvenile European seabass Dicentrarchus labrax in resonse to ocean acidification and warming
2022
Ongoing climate change is leading to warmer and more acidic oceans. The future distribution of fish within the oceans depends on their capacity to adapt to these new environments. Only few studies have examined the effects of ocean acidification (OA) and warming (OW) on the metabolism of long-lived fish over successive generations. We therefore aimed to investigate the effect of OA on larval and juvenile growth and metabolism on two successive generations of European sea bass (Dicentrarchus labrax L.) as well as the effect of OAW on larval and juvenile growth and metabolism of the second generation. European sea bass is a large economically important fish species with a long generation time…