6533b7d3fe1ef96bd125ff93
RESEARCH PRODUCT
Highly efficient transport of carboxyfluorescein diacetate succinimidyl ester into COS7 cells using human papillomavirus-like particles
Martin SappChristian BergsdorfCarsten BeyerViktor UmanskyMargaret Werrsubject
Human papillomavirusVirosomesvirusesDrug delivery systemCellBiophysicsSuccinimidesCarboxyfluorescein diacetate succinimidyl esterBiologyAntibodies Viralcomplex mixturesBiochemistrylaw.inventionFlow cytometrychemistry.chemical_compoundCapsidVirus-like particleStructural BiologylawIn vivoGeneticsmedicineAnimalsMolecular BiologyFluorescent Dyesmedicine.diagnostic_testVirionvirus diseasesBiological TransportOncogene Proteins ViralCell BiologyFluoresceinsFluorescenceIn vitromedicine.anatomical_structurechemistryBiochemistryCOS CellsRecombinant DNACapsid ProteinsVirus-like particleFluorescence labellingdescription
AbstractHuman papillomavirus virus-like particles (VLPs) have recently been used to deliver genes into mammalian cells in vitro and in vivo. Here, we investigated whether VLPs may serve as an efficient carrier of low molecular weight compounds (e.g. hormones, vitamins, peptides etc.) into cells. COS7 cells were incubated with recombinant HPV-16L1/L2 VLPs labelled with the fluorescence dye carboxyfluorescein diacetate succinimidyl ester. Using flow cytometry, we demonstrate that labelled VLPs can specifically bind to the cell surface followed by their complete internalisation. Our results indicate that VLPs are promising vehicles for highly efficient delivery of low molecular weight compounds into cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-01-22 | FEBS Letters |