Search results for " Laboratory"

showing 10 items of 622 documents

Unraveling the role of the secretor antigen in human rotavirus attachment to histo-blood group antigens

2019

25 Páginas, 7 figuras, 2 tablas

RNA virusesRotavirusViral DiseasesPhysiologyViral Nonstructural ProteinsPathology and Laboratory MedicineCrystallography X-Raymedicine.disease_causeBiochemistryBinding AnalysisReovirusesImmune PhysiologyRotavirusMedicine and Health SciencesChemical PrecipitationBiology (General)Antigens ViralGastroenterologiachemistry.chemical_classification0303 health sciencesImmune System ProteinsCrystallographyMolecular StructurebiologyPhysics030302 biochemistry & molecular biologyChemical ReactionsRNA-Binding ProteinsCondensed Matter PhysicsLigand (biochemistry)Amino acidChemistryInfectious DiseasesMedical MicrobiologyViral PathogensVirusesPhysical SciencesCrystal StructurePathogensCrystallizationResearch ArticleChemical ElementsGlycanQH301-705.5Virus RNAViral proteinImmunologyResearch and Analysis MethodsMicrobiologyABO Blood-Group SystemCell Line03 medical and health sciencesAntigenVirologyGeneticsmedicineSolid State PhysicsHumansAntigensBinding siteMicrobial PathogensMolecular BiologyRotavirus InfectionChemical Characterization030304 developmental biologyChemical PhysicsBinding SitesBiology and life sciencesMutagenesisOrganismsProteinsRC581-607Molecular biologyCarbonchemistrybiology.proteinCapsid ProteinsParasitologyImmunologic diseases. AllergyPLOS Pathogens
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Investigation of superspreading COVID-19 outbreak events in meat and poultry processing plants in Germany: A cross-sectional study.

2020

Since May 2020, several COVID-19 outbreaks have occurred in the German meat industry despite various protective measures, and temperature and ventilation conditions were considered as possible high-risk factors. This cross-sectional study examined meat and poultry plants to examine possible risk factors. Companies completed a self-administered questionnaire on the work environment and protective measures taken to prevent SARS-CoV-2 infection. Multivariable logistic regression analysis adjusted for the possibility to distance at least 1.5 meters, break rules, and employment status was performed to identify risk factors associated with COVID-19 cases. Twenty-two meat and poultry plants with 1…

RNA virusesViral DiseasesMeat packing industryCross-sectional studyEconomicsCoronavirusesSocial SciencesLogistic regressionPoultrylaw.inventionDisease OutbreaksMedical ConditionslawRisk FactorsAnimal ProductsGermanyMedicine and Health SciencesMedicineFood IndustryPublic and Occupational HealthWorkplacePathology and laboratory medicineVirus TestingMultidisciplinaryPhysicsQRTemperatureClassical MechanicsEukaryotaAgriculturePoultry farmingMedical microbiologyDynamicsMeat ProductsInfectious DiseasesAir FlowVentilation (architecture)VirusesPhysical SciencesVertebratesMedicineSARS CoV 2PathogensResearch ArticleEmploymentPhysics - Physics and SocietyMeatCoronavirus disease 2019 (COVID-19)SARS coronavirusScienceFOS: Physical sciencesPhysics and Society (physics.soc-ph)MicrobiologyBirdsAerodynamicsDiagnostic MedicineEnvironmental healthHumansAnimalsNutritionbusiness.industrySARS-CoV-2OrganismsViral pathogensOutbreakCOVID-19Biology and Life SciencesCovid 19Odds ratioPhysics - Medical PhysicsVentilationDietMicrobial pathogensCross-Sectional StudiesFoodLabor EconomicsAmniotesMedical Physics (physics.med-ph)businessZoologyPloS one
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Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineage

2021

The accessory protein Nef of human and simian immunodeficiency viruses (HIV and SIV) is an important pathogenicity factor known to interact with cellular protein kinases and other signaling proteins. A canonical SH3 domain binding motif in Nef is required for most of these interactions. For example, HIV-1 Nef activates the tyrosine kinase Hck by tightly binding to its SH3 domain. An archetypal contact between a negatively charged SH3 residue and a highly conserved arginine in Nef (Arg77) plays a key role here. Combining structural analyses with functional assays, we here show that Nef proteins have also developed a distinct structural strategy—termed the "R-clamp”—that favors the formation …

RNA virusesviruksetvirusesSimian Acquired Immunodeficiency SyndromeHIV InfectionsPathology and Laboratory MedicineSH3 domainWhite Blood CellsImmunodeficiency VirusesAnimal CellsMedicine and Health SciencesBiology (General)MammalsGenetics11832 Microbiology and virology0303 health sciencesKinase030302 biochemistry & molecular biologyEukaryotavirus diseasesTransfection3. Good healthSIVMedical MicrobiologyViral PathogensViral evolutionVirusesVertebratesProto-Oncogene Proteins c-hckApesSimian Immunodeficiency VirusPathogensCellular TypesTyrosine kinaseResearch ArticlePrimateskinaasitEvolutionary ImmunologyLineage (genetic)QH301-705.5Immune CellsImmunologyevoluutioBiologyTransfectionResearch and Analysis MethodsHIV-tartuntaMicrobiologyViral EvolutionEvolution Molecularsrc Homology Domains03 medical and health sciencesVirologyRetrovirusesGeneticsAnimalsHumansLuciferaseAmino Acid Sequencenef Gene Products Human Immunodeficiency VirusChimpanzeesMolecular Biology TechniquesMicrobial PathogensMolecular Biology030304 developmental biologyEvolutionary BiologyBlood CellsSequence Homology Amino AcidMacrophagesLentivirusOrganismsBiology and Life SciencesHIVCell BiologyRC581-607Organismal Evolution3121 General medicine internal medicine and other clinical medicineMicrobial EvolutionAmniotesHIV-1ParasitologySalt bridgeproteiinitImmunologic diseases. AllergyZoology
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Radon and material radiopurity assessment for the NEXT double beta decay experiment

2015

Artículo escrito por un elevado número de autores, solo se referencian el que aparece en primer lugar, el nombre del grupo de colaboración, si le hubiere, y los autores pertenecientes a la UAM

Radon mitigationPhysics - Instrumentation and DetectorsNuclear engineeringchemistry.chemical_elementFOS: Physical sciencesRadon7. Clean energy01 natural sciencesHigh Energy Physics - ExperimentHigh Energy Physics - Experiment (hep-ex)XenonCambres d'ionitzacióDouble beta decayGamma detectors (HPGe)0103 physical sciencesGamma spectroscopyNuclear Experiment (nucl-ex)010306 general physicsNuclear ExperimentDetectors de radiació010308 nuclear & particles physicsIonization chambersCanfranc Underground LaboratoryMaterial radiopurityFísicaTime-Projection Chamber (TPC)Instrumentation and Detectors (physics.ins-det)Double beta decaySemiconductor detectorchemistry13. Climate actionNuclear countersEnvironmental scienceNeutrino
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The ubiquitin-specific protease USP8 is critical for the development and homeostasis of T cells

2015

The modification of proteins by ubiquitin has a major role in cells of the immune system and is counteracted by various deubiquitinating enzymes (DUBs) with poorly defined functions. Here we identified the ubiquitin-specific protease USP8 as a regulatory component of the T cell antigen receptor (TCR) signalosome that interacted with the adaptor Gads and the regulatory molecule 14-3-3β. Caspase-dependent processing of USP8 occurred after stimulation of the TCR. T cell-specific deletion of USP8 in mice revealed that USP8 was essential for thymocyte maturation and upregulation of the gene encoding the cytokine receptor IL-7Rα mediated by the transcription factor Foxo1. Mice with T cell-specifi…

Regulatory T cellT-LymphocytesImmunologyReceptors Antigen T-Cell610 Medicine & healthBiologyCD8-Positive T-LymphocytesJurkat cellsJurkat CellsMiceddc:570EndopeptidasesmedicineImmunology and AllergyAnimalsHomeostasisHumans10239 Institute of Laboratory Animal ScienceIL-2 receptorAdaptor Proteins Signal Transducing2403 ImmunologyReceptors Interleukin-7ThymocytesEndosomal Sorting Complexes Required for TransportForkhead Box Protein O1ZAP70T-cell receptorCD28Cell DifferentiationForkhead Transcription FactorsColitisCell biologyThymocytemedicine.anatomical_structure2723 Immunology and Allergy570 Life sciences; biology590 Animals (Zoology)Ubiquitin ThiolesteraseCD8
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From Laboratory Mixes to Full Scale Test: Rutting Evaluation of Bio-recycled Asphalt Mixes

2021

The present paper describes the rutting behavior of innovative mixes incorporating 50% of Reclaimed Asphalt (RA) with bio-materials. They were assessed in the laboratory and in a full-scale accelerated experiment. The innovative mixes studied here contained bio-materials especially designed to help recycling by re-activating the aged binder from RA. Four mixes were evaluated: three of them are manufactured with bio-materials, (two bio-rejuvenators and one bio-binder) and one was a control mix, which was a high modulus asphalt mix (EME2). In this study, the rutting resistance of the four mixes was first evaluated in the laboratory with both European and US methods. The full-scale test was th…

RutAsphaltSettore ICAR/04 - Strade Ferrovie Ed AeroportiEnvironmental scienceGeotechnical engineeringFull scale testAccelerated pavement test Bio-materials Laboratory test Reclaimed asphalt Rutting test
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Recovery Estimation of Dried Foodborne Pathogens Is Directly Related to Rehydration Kinetics.

2016

International audience; Drying is a common process which is used to preserve food products and technological microorganisms, but which is deleterious for the cells. The aim of this study is to differentiate the effects of drying alone from the effects of the successive and necessary rehydration. Rehydration of dried bacteria is a critical step already studied in starter culture but not for different kinetics and not for pathogens. In the present study, the influence of rehydration kinetics was investigated for three foodborne pathogens involved in neonatal diseases caused by the consumption of rehydrated milk powder: Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enteri…

Salmonella typhimuriumBacterial Diseases0301 basic medicineSurvivalPhysiologyMicroorganism[ SDV.AEN ] Life Sciences [q-bio]/Food and NutritionCell MembranesResistancelcsh:MedicineEscherichia-coliPathology and Laboratory MedicineLactic Acid BacteriaFoodborne OrganismsSalmonellaMedicine and Health SciencesFood scienceProkaryoteslcsh:SciencemembraneMultidisciplinarybiologyDehydrationEnteric BacteriaSalmonella entericaBacterial InfectionsAnhydrobiosisBacterial PathogensDeathInfectious DiseasesMedical MicrobiologySalmonella entericaPathogensCellular Structures and OrganellesResearch ArticleWater activityDesiccation tolerance030106 microbiologyMicrobiologyMicrobiology03 medical and health sciencesCronobacter sakazakiiEnterobacteriaceaemedicineHumansDehydrationDesiccationMicrobial PathogensBacteriabusiness.industrylcsh:ROrganismsFood ConsumptionBiology and Life SciencesCell Biologymedicine.diseasebiology.organism_classificationFood safetyCronobacter sakazakiiInfant formulaFood MicrobiologyFluid Therapylcsh:QPhysiological ProcessesbusinessBacteriaWater activityPLoS ONE
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An interlaboratory study as useful tool for proficiency testing of chemical oxygen demand measurements using solid substrates and liquid samples with…

2009

In 2008, the first Proficiency Testing Scheme of Chemical Oxygen Demand (1stCOD-PTADG) was conducted to assess the results obtained for different research groups whose field work is mainly anaerobic digestion. This study was performed using four samples, two solid samples as raw materials and two solid samples to prepare high concentration suspended solid solutions. Invitations were sent to a large number of laboratories, mainly to anaerobic digestion research groups. Finally, thirty labs from sixteen countries agreed to participate, but for different reasons four participants could not send any data. In total, twenty-six results were reported to the COD-PT coordinator. This study showed th…

Sample (material)[SDV]Life Sciences [q-bio]Analytical chemistryCarbohydratesSolid substrateSuspended solidsHomogeneity test010501 environmental sciences01 natural sciencesStandard deviationChemistry Techniques AnalyticalAnalytical ChemistryProficiency testingFatsAnaerobic digestionStatisticsProficiency testingPlant OilsSunflower OilAnaerobiosisChenopodium quinoa0105 earth and related environmental sciencesSuspended solidsInterlaboratory studyScience & TechnologySewageChemistryClinical Laboratory Techniques010401 analytical chemistryChemical oxygen demandProteinsReproducibility of ResultsCHEMICAL OXYDEN DEMANDReference StandardsTheoretical oxygen demand0104 chemical sciencesOxygenCHIMIE ANALYTIQUECertified reference materials[SDE]Environmental SciencesChemical oxygen demandRound robin test
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Implementasi Pembelajaran Fisika Berbasis Laboratorium Virtual terhadap Keterampilan Proses Sains dan Persepsi Mahasiswa

2018

Penelitian ini bertujuan untuk mengetahui penerapan pembelajaran fisika berbasis media laboratorium virtual , Keterampilan Proses Sains (KPS), dan persepsi mahasiswa pada mata kuliah fisika dasar. Metode penelitian menggunakan pre-eksperimental design dan teknik purposive sample digunakan untuk memilih sampel yaitu kelas pendidikan fisika dan pendidikan matematika Universitas Papua. Instrumen dalam penelitian ini yaitu lembar observasi dan penilaian KPS serta angket persepsi mahasiswa yang dinilai di akhir pembelajaran. Hasil penelitian menunjukkan persentase KPS yang diobservasi selama perkuliahan 86,4% ± SD 12,7 meliputi aspek keterampilan menggunakan alat, mengambil data, dan mengkomunik…

Science Process SkillsPersepsiPerceptionGeneral MedicineKeterampilan Proses Sainslcsh:LLaboratorium VirtualVirtual Laboratorylcsh:EducationBerkala Ilmiah Pendidikan Fisika
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Determination of organic pollutants in Anguilla anguilla by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)

2021

© 2021 The Authors.

ScienceClinical BiochemistryIllicit drugs010501 environmental sciencesQuechersTandem mass spectrometry01 natural sciences03 medical and health sciencesEmerging pollutantsLiquid chromatography–mass spectrometryPFASsPlaguicidesSolid phase extractionPesticides030304 developmental biology0105 earth and related environmental sciencesPollutant0303 health sciencesChromatographyChemistryExtraction (chemistry)QQuímicaQueChERsContaminationPesticideEMR-lipidMedical Laboratory TechnologyLiverPharmaceuticalsMuscleMethodsX
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