Search results for " Local"

Signal sequences modulate the immunogenic performance of human hepatitis C virus E2 gene

2005

Abstract Envelope protein E2 of human hepatitis C virus (HCV) is an attractive component of a prototype HCV vaccine. Delivered by DNA immunogens, E2 evokes specific immune response of Th1-type, failing to induce either considerable antibody production, or T-helper cell proliferation. We aimed at modulating the immunogenic performance of E2 gene by changing the mode of protein expression in eukaryotic cells. Plasmids were constructed encoding full-length E2 and nonstructural protein 1 (p7) fused to either 13 or 38 C-terminal amino acids (aa) of HCV E1 that contain second hydrophobic segment of E1 stop-transfer signal, or a complete E1 stop-transfer signal with duplicated second hydrophobic s…

Expression and cellular localization of the Nef protein from human immunodeficiency virus-1 in stably transfected B-cells.

1992

Nef protein, encoded by the regulatory nef gene of human immunodeficiency virus type 1 (HIV-1), was expressed in the B-cell line Raji. The cells were stably transfected with plasmids containing the nef transcriptional cassette. They expressed Nef with an Mr of 27,000; the yield could be augmented by incubation with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate. The intracellular localization of Nef was analyzed applying immunofluorescence microscopy using a confocal laser scanning microscope. The antigen was stained with a monoclonal antibody directed against the N-terminal part of Nef. The experiments revealed that in non-dividing cells Nef is present both in the cytoplasm and th…

Coverage analysis of RFID indoor localization system for refrigerated warehouses based on 2D-ray tracing

2012

New insights in chemical reactivity from quantum chemical topology.

2021

International audience; Based on the quantum chemical topology of the modified electron localization function ELFx, an efficient and robust mechanistic methodology designed to identify the favorable reaction pathway between two reactants is proposed. We first recall and reshape how the supermolecular interaction energy can be evaluated from only three distinct terms, namely the intermolecular coulomb energy, the intermolecular exchange‐correlation energy and the intramolecular energies of reactants. Thereafter, we show that the reactivity between the reactants is driven by the first‐order variation in the coulomb intermolecular energy defined in terms of the response to changes in the numbe…

The Reshaping of the City-Port Interface in Palermo: A Case of Successful Urban Governance?

2023

In the last decades, various port cities in western countries have developed policies to make shipping activities more compatible with the urban environment and organization. This paper provides a critical interpretation of the process that is leading to the regeneration of the waterfront area of Palermo, the Italian fifth largest city by population. In a first part of the paper the events and economic factors that have negatively impacted on the city-port spatial relations are outlined. Subsequently, in the light of the new governance relations established between the city and port authorities, the strategy and main interventions of the port masterplan that is currently reshaping the city-…

Rotationally symmetric 1-harmonic flows from D2 TO S 2: Local well-posedness and finite time blowup

2010

The 1-harmonic flow from the disk to the sphere with constant Dirichlet boundary conditions is analyzed in the case of rotational symmetry. Sufficient conditions on the initial datum are given, such that a unique classical solution exists for short times. Also, a sharp criterion on the boundary condition is identified, such that any classical solution will blow up in finite time. Finally, nongeneric examples of finite time blowup are exhibited for any boundary condition.

A high-temperature x-ray absorption spectroscopy study of

1996

We present for the first time a high-temperature x-ray absorption spectroscopy study of at the edge. The analysis both of the XANES and of the EXAFS regions shows that, in , a progressive localization of the 5d states following the temperature rise is accompanied at T > 350 K by a distortion of the octahedra, which increases up to the decomposition temperature . The distortion leads to the lowering of the rhenium site symmetry from and is due to the electron - phonon coupling of the 5d electrons with the and phonons and to the Jahn - Teller effect caused by the rhenium electron which becomes localized at high temperature.

X-ray absorption spectroscopy study of local dynamics and thermal expansion in ReO3

2015

The thermal expansion of polycrystalline ${\mathrm{ReO}}_{3}$ is studied in vacuum by x-ray diffraction from 300 to 600 K and by extended x-ray absorption fine structure (EXAFS) at the Re ${L}_{3}$ edge from 30 to 600 K. A detailed EXAFS analysis is presented up to the sixth coordination shell around Re. The crystal thermal expansion is weak throughout the explored range, negative below 100 K, positive from 150 to 500 K, and negative again above 500 K. The analysis of mean square relative displacements obtained by EXAFS and of mean square displacements available from x-ray and neutron diffraction measurements confirms the presence of significant static disorder in the investigated samples. …

Le festival des Nuits de Bourgogne. Décentralisation artistique, militantisme et politiques culturelles locales (1954-1984)

2012

National audience; …

Preprocessing of region of interest localization based on local surface curvature analysis for three-dimensional reconstruction with multiresolution

2009

We present an approach to integrate a preprocessing step of the region of interest ROI localization into 3-D scanners laser or ste- reoscopic. The definite objective is to make the 3-D scanner intelligent enough to localize rapidly in the scene, during the preprocessing phase, the regions with high surface curvature, so that precise scanning will be done only in these regions instead of in the whole scene. In this way, the scanning time can be largely reduced, and the results contain only per- tinent data. To test its feasibility and efficiency, we simulated the prepro- cessing process under an active stereoscopic system composed of two cameras and a video projector. The ROI localization is…