Search results for " Mapping"

Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns.

1990

A major cytoskeletal polypeptide (Mr approximately 46,000; protein IT) of human intestinal epithelium was characterized by biochemical and immunological methods. The polypeptide, which was identified as a specific and genuine mRNA product by translation in vitro, reacted, in immunoblotting after SDS-PAGE, only with one of numerous cytokeratin (CK) antisera tested but with none of many monoclonal CK antibodies. In vitro, it formed heterotypic complexes with the type II CK 8, as shown by blot binding assays and gel electrophoresis in 4 M urea, and these complexes assembled into intermediate filaments (IFs) under appropriate conditions. A chymotrypsin-resistant Mr approximately 38,000 core fra…

Mapping floristic diversity: a case study in Sicily

2021

This paper presents an operational methodology to map and analyze the floristic richness of “target species” in Natura 2000 sites, making use of G.I.S. tools and procedures. A Floristic diversity map (scale 1:50,000), covering an area of 612 km2, was produced by a team of experts as part of the management plans of “Madonie Mountains” Sites of Community Importance (SCIs), located in Sicily (Italy). The primary grid map represents the richness of “target species”, which include species of Community interests, taxa on the National Red List, endemic and threatened, species protected under International Conventions, taxa of phytogeographic importance. Secondary data frames include a three-dimens…

Agricultural landscapes and biodiversity conservation: a case study in Sicily (Italy)

2015

The diversity of life is made up not only of the diversity of plants and animal species, habitats and ecosystems, but also of the diversity of human cultures. These diversities interact with one another in complex ways and express the mutual adaptation between humans and the environment at local level. Sicilian traditional agro-ecosystems, due to the history of the territory and the resulting social and economic context, are configured in a heterogeneous mosaic rich in residual features of environmental value, which enhance the connectivity of the ecological network and support a high proportion of species that are rare or of conservation concern. As a case study we analyzed the characteris…

Emotions in Economic Decision Making: A Multidisciplinary Approach

2013

AbstractAccording to classical and neoclassical economics, decisions are made based on information and cost-benefit analysis. In reality, the decision making process is much more complex than previously thought, because it also involves psychological factors. Decision making is interdisciplinary, researched by psychologists, sociologists, economists, philosophers, neuroscientists and others. These fields have distinctive and common concepts about decision making. The aim of this paper is to identify what role emotions play in the economic decision making process. The paper focuses on describing and explaining the interconnection of sciences, such as economics, psychology and neuroscience, b…

Modeling the influence of alternative forest management scenarios on wood production in the mediterranenan region. The MiMoSe approach.

2014

Ecosystem services (ES) are defined as the benefits that human obtain from ecosystems. Forest ecosystems provide many essential goods and services, such as flood and landslide control, erosion prevention and maintenance of soil fertility, water and air quality, biodiversity, wildlife habitat, recreational space for humans and aesthetic appreciation of natural landscapes, wood for fuel, construction and fibre, biomass for energy, and non-timber forest products. The quality and quantity of these goods and services are influenced by forest management, especially in the Mediterranean area where the forest has been exploited for a long time. In this study, we propose a method (MiMoSe- Multiscale…

Characterization of murine monoclonal antibodies against the Ro52 autoantigen

1997

SUMMARY Immunization of BALB/c mice with purified recombinant human Ro52 protein resulted in three anti-Ro52 MoAbs termed 2E7, 4C6 and 4F11. All anti-Ro52 MoAbs specifically reacted with recombinant human Ro52 protein, and also with Ro52 protein in total extracts of all human cell lines analysed, including the epithelial cell line HeLa, the B cell line Raji, the bladder carcinoma cell line RT112, and a fibroblast cell line derived from patients with xeroderma pigmentosum. The anti-Ro52 MoAbs were able to immunoprecipitate the recombinant human Ro52 protein expressed in wheat germ extract, but failed to precipitate hY RNAs from cell extracts. The staining pattern of the MoAbs strongly differ…

Assisting Electrophysiological Substrate Quantification in Atrial Fibrillation Ablation

2019

[EN] Catheter ablation (CA) is the most popular treatment of atrial fibrillation (AF) with good results in paroxysmal AF, while its efficiency is significantly reduced in persistent AF. With the equipment used for CA strongly depending on electro-gram (EGM) fractionation quantification, the use of a reliable fractionation estimator is crucial to reduce the high recurrence rates in persistent AF. This work introduces a non-linear EGM fractionation quantification technique, which is based on coarse-grained correlation dimension (CGCD) computed over epochs of 1 second. Recordings were firstly normalized, denoised and lowpass filtered. The final CGCD value was calculated by the median CGCD valu…

Phylogenetic reconstruction of the yeast genus Kluyveromyces: restriction map analysis of the 5.8S rRNA gene and the two ribosomal internal transcrib…

1998

Summary We have constructed restriction site maps of the 5.8S rRNA gene and the two ITS regions in 60 strains of Kluyveromyces genus. We test the value of this region as a phylogenetic indicator, and its possible use as a fast and easy method to identify species of this genus. Despite some minor incongruences, our results are in good agreement with previous phylogenetic reconstructions based on the 18S rRNA gene sequencing (Cai et al., 1996; James et al., 1997). A highly significant monophyletic group was formed by K. lactis, K. marxianus, K. aestuarii, K. dobzhanskii and K. wickerhamii, which should be considered the true Kluyveromyces genus. The other species of the genus were grouped wit…

Comments on “New epicardial mapping electrode with warming/cooling function for experimental electrophysiology studies” [33 (5) (2011) 653–659]

2012

Personalization of Fast Conduction Purkinje System in Eikonal-Based Electrophysiological Models with Optical Mapping Data

2010

We present a pipeline for the personalization of model-based Purkinje fast conduction system using fast electrophysiological models and optical mapping data acquired from ex-vivo porcine hearts. The regional density of the Purkinje terminals as well as the latest endocardial activation time were the parameters personalized in an iterative procedure maximizing the similarity between the outcome of the electrophysiological simulations and measurements obtained from optical mapping data. We used a fast wave-front Eikonal-based electrophysiological model that generated the depolarization time maps that were subsequently compared with measurements at each iteration of the optimization stage. The…