Search results for " Mapping"
showing 10 items of 1411 documents
All-Food-Seq (AFS): a quantifiable screen for species in biological samples by deep DNA sequencing.
2013
Background DNA-based methods like PCR efficiently identify and quantify the taxon composition of complex biological materials, but are limited to detecting species targeted by the choice of the primer assay. We show here how untargeted deep sequencing of foodstuff total genomic DNA, followed by bioinformatic analysis of sequence reads, facilitates highly accurate identification of species from all kingdoms of life, at the same time enabling quantitative measurement of the main ingredients and detection of unanticipated food components. Results Sequence data simulation and real-case Illumina sequencing of DNA from reference sausages composed of mammalian (pig, cow, horse, sheep) and avian (c…
L-Band radiative properties of vine vegetation at the MELBEX III SMOS Cal/Val Site
2012
Radiative properties at 1.4 GHz of vine vegetation are investigated by measuring brightness temperatures with the ETH L-band Radiometer II (ELBARA II) operated on a tower at the Mediterranean Ecosystem L-band Characterisation Experiment III (MELBEX III) field site in Spain. To this aim, experiments with and without a reflecting foil placed under the vines were performed for the vegetation winter and summer states, respectively, to provide prevailingly information on vegetation transmissivities. The resulting parameters, which can be considered as "ground truth" for the MELBEX III vineyard, were retrieved from brightness temperature at horizontal and vertical polarization measured at observa…
Comparative study of the environmental footprints of marinas on European Islands
2021
AbstractPorts have been key elements in Europe's economic development. This situation is even more relevant on islands, which are highly dependent on the maritime sector. Consequently, over the years, ports with diverse functionalities have been established both in mainland Europe and on its outlying islands. This article discusses the environmental impact of leisure marinas on European islands, especially as they are closely linked to economic development through tourism. The aim is to study the environmental impact of these infrastructures by determining the carbon and water footprints of marinas on European islands in the Atlantic and the Mediterranean. The results obtained enable the au…
Integration of different cardiac electrophysiological models into a single simulation pipeline
2012
Clinical translation of computational models of the heart has been hampered by the absence of complete and rigorous technical and clinical validation, as well as benchmarking of the developed tools. To address this issue, a dataset containing the cardiac anatomy and fibre orientations from magnetic resonance images (MRI), as well as epicardial transmembrane potentials from optical mapping acquired on ex-vivo porcine hearts, have previously been made available to the community. Image processing techniques were developed to integrate MRI images with electrical information. Different models were tested and compared with the integrated data1, including: i) a new methodology to customize and reg…
Anti-ADAMTS13 autoantibody profiling in patients with immune-mediated thrombotic thrombocytopenic purpura.
2021
Anti-A Disintegrin and Metalloproteinase with a ThromboSpondin type 1 motif, member 13 (ADAMTS13) autoantibodies cause a severe ADAMTS13 deficiency in immune-mediated thrombotic thrombocytopenic purpura (iTTP). ADAMTS13 consists of a metalloprotease (M), a disintegrin-like (D) domain, 8 thrombospondin type 1 repeats (T1-T8), a cysteine-rich (C), a spacer (S), and 2 CUB domains (CUB1-2). We recently developed a high-throughput epitope mapping assay based on small, nonoverlapping ADAMTS13 fragments (M, DT, CS, T2-T5, T6-T8, CUB1-2). With this assay, we performed a comprehensive epitope mapping using 131 acute-phase samples and for the first time a large group of remission samples (n = 50). Ne…
Fixed point results for $r$-$(\mathbf{\eta},\xi,\psi)$-contractive mappings of type (I), (II) and (III)
2013
In this paper, we introduce some classes of $r$-$(\eta,\xi,\psi)$-contractive mappings and prove results of fixed point in the setting of complete metric spaces. Some examples and an application to integral equations are given to illustrate the usability of the obtained results.
Specific DNA probes to detect Escherichia coli strains producing cytotoxic necrotising factor type 1 or type 2
1994
Cytotoxic necrotising factors type 1 (CNF1) and type 2 (CNF2) are produced by many Escherichia coli strains isolated from man and animals with intestinal or extra-intestinal colibacillosis. In most laboratories, CNF-producing strains are detected by a cell cytotoxicity assay and confirmed with a neutralisation assay or a mouse footpad assay. In this study, we sought to determine whether DNA probes could detect clinical isolates of E. coli producing CNF2 or CNF1, or both, without the need for cell cultures or animal assays. Two internal fragments of the gene encoding CNF2 were used as DNA probes: a 875-bp XhoI-PstI DNA fragment and an adjacent 335-bp PstI-ClaI fragment. A positive response w…
Cloning of Clostridium difficile toxin B gene and demonstration of high N-terminal homology between toxin A and B.
1990
High titered Clostridium sordellii lethal toxin antiserum, cross-reactive with C. difficile cytotoxin B (ToxB), was used to isolate toxB fragments from a C. difficile expression library. Recombinant clones containing toxB fragments of the 5' and 3' end were isolate. A 2.5-kb HincII fragment of chromosomal DNA overlaps both groups of clones. A partial restriction map of the total toxB gene is presented. The gene is positioned upstream of utxA and toxA, toxB has a size of 6.9 kb, corresponding to a 250-kDa polypeptide. A partial sequence of the 5' end of toxB was determined. The sequence contains 398 bp upstream of toxB with a putative Shine-Dalgarno box (AGGAGA) and 609 bp of the toxB open r…
Characterizations of adenovirus type 41 isolates from children with acute gastroenteritis in Japan, Vietnam, and Korea.
2004
ABSTRACT Genetic and antigenic characterizations of 70 strains of adenovirus type 41 (Ad41), isolated between 1998 and 2001 from children in Japan, Vietnam, and Korea, were done by DNA restriction enzyme (RE) analysis, sequencing analysis, and monoclonal antibody (MAb)-based enzyme-linked immunosorbent assay (ELISA). Eight genome types were observed in the present study, among which D25, D26, D27, and D28 were novel genome types. These eight genome types were divided into two genome-type clusters (GTCs) based on phylogenetic analysis of the hypervariable regions (HVRs) of the hexon. GTC1 includes D1, D25, D26, D27, and D28, and the GTC2 contains D4, D12, and D22. The amino acid homologies a…
Application of total internal reflexion fluorescence microscopy for studying pH changes in an occluded electrochemical cell: Development of a wavegui…
2006
A device for pH mapping derived from optical sensors similar to total internal reflection fluorescence microscopy (TIRFM) has been developed for future possible applications in the field of localized corrosion. The sensing principle is the increase of the fluorescent yield of the fluorescein with the pH of the medium. The basic principle of this sensor is based on the excitation of a fluorescent silica polymer film of nanometre dimensions, deposited by sol–gel method on a waveguiding layer. The total internal reflexion conditions creates an evanescent wave which interacts with the molecules trapped in the silica layer. A conventional microscope located above the sample collects the fluoresc…