Search results for " Mito"

showing 10 items of 895 documents

Analyse de la variabilité génétique mitochondriale et chloroplastique de deux espèces du genre Lupinus (L.Albus et L. Mutabilis)

1988

Notice présente dans BelInra (https://belinra.inra.fr/gestion/catalog.php?categ=isbd&id=90015); il s'agit d'un type de produit dont les métadonnées ne correspondent pas aux métadonnées attendues dans les autres types de produit : DISSERTATION; Analyse de la variabilité génétique mitochondriale et chloroplastique de deux espèces du genre Lupinus (L.Albus et L. Mutabilis)

amélioration génétiquechloroplaste adn[ SDV.BV ] Life Sciences [q-bio]/Vegetal Biologypurificationrefininglupinus mutabilissolvent free microwave extraction (sfem)méthode d'analysetarwiplante fourragèrelupinus albusplasmidelectrophoresisplasmideextraction[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyélectrophorèseADN mitochondrial
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Sea urchin deciliation induces thermoresistance and activates the p38 mitogen-activated protein kinase pathway.

2003

In this study, we demonstrate by a variety of approaches (ie, morphological analysis, Western blots, immunolocalization, and the use of specific antibodies) that hyperosmotic deciliation stress of sea urchin embryos induces a thermotolerant response. Deciliation is also able to activate a phosphorylation signaling cascade the effector of which might be the p38 stress-activated protein kinase because we found that the administration of the p38 inhibitor SB203580 to sea urchin deciliated gastrula embryos makes the hyperosmotic deciliation stress lethal.

animal structuresHot TemperaturePyridinesp38 mitogen-activated protein kinasesSEA URCHIN DECILIATION p38MAP KINASEBiochemistryp38 Mitogen-Activated Protein KinasesEnzyme activatorStress Physiologicalbiology.animalAnimalsCiliaSettore BIO/06 - Anatomia Comparata E CitologiaPhosphorylationProtein kinase ASea urchinbiologyEffectorImidazolesAntibodies MonoclonalCell BiologyGastrulaOriginal ArticlesMolecular biologyBlotEnzyme ActivationSea Urchinsembryonic structuresPhosphorylationElectrophoresis Polyacrylamide GelSignal transductionMitogen-Activated Protein KinasesSignal TransductionCell stresschaperones
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Cabut, a C2H2 zinc finger transcription factor, is required during Drosophila dorsal closure downstream of JNK signaling.

2005

AbstractDuring dorsal closure, the lateral epithelia on each side of the embryo migrate dorsally over the amnioserosa and fuse at the dorsal midline. Detailed genetic studies have revealed that many molecules are involved in this epithelial sheet movement, either with a signaling function or as structural or motor components of the process. Here, we report the characterization of cabut (cbt), a new Drosophila gene involved in dorsal closure. cbt is expressed in the yolk sac nuclei and in the lateral epidermis. The Cbt protein contains three C2H2-type zinc fingers and a serine-rich domain, suggesting that it functions as a transcription factor. cbt mutants die as embryos with dorsal closure …

animal structuresMorphogenesisBiologyCabutZinc fingerMorphogenesismedicineAnimalsDrosophila ProteinsDorsal closureYolk sacMolecular BiologyTranscription factorYolk nucleiCytoskeletonGeneticsZinc fingerEpidermis (botany)C2H2 Zinc FingerJNK Mitogen-Activated Protein KinasesZinc FingersCell BiologyDorsal closureCell biologymedicine.anatomical_structureDrosophila melanogasterEpidermal Cellsembryonic structuresMutationJNK cascadeDrosophilaJNKDevelopmental BiologySignal TransductionTranscription FactorsDevelopmental biology
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Autophagy

2012

Klionsky, Daniel J. et al.

autophagy assays[SDV]Life Sciences [q-bio]AutolysosomeAutophagosome maturationautophagosomeBioinformaticsstressChaperone-mediated autophagyModelsLC3MESH: Animalsguidelinesautolysosome autophagosome flux LC3 lysosome phagophore stress vacuoleSettore BIO/06 - Anatomia Comparata E CitologiaComputingMilieux_MISCELLANEOUSSettore BIO/17Autophagy databaseautolysosome3. Good healthddc:540lysosomeEnergy and redox metabolism Mitochondrial medicine [NCMLS 4]methods [Biological Assay]Biological AssaySettore BIO/17 - ISTOLOGIANeuroniMAP1LC3BHumanautophagygenetics [Autophagy]AutofagiaMESH: Autophagy*/genetics[SDV.BC]Life Sciences [q-bio]/Cellular BiologyAutofagia; Neuroni; istologiaBiologyModels BiologicalLC3; autolysosome; autophagosome; flux; lysosome; phagophore; stress; vacuoleddc:570AutophagyAnimalsHumansAutophagy-Related Protein 7[SDV.BC] Life Sciences [q-bio]/Cellular BiologyBiological Assay/methodsMolecular BiologyBiologyAutophagy; guidelines; autophagy assaysistologiaphagophoreMESH: HumansAnimals; Biological Assay; Humans; Models Biological; AutophagyvacuoleAnimal[ SDV.BC ] Life Sciences [q-bio]/Cellular BiologyMESH: Models BiologicalPathogenesis and modulation of inflammation Infection and autoimmunity [N4i 1]Cell BiologyBiologicalAutophagy/geneticsfluxAutophagosome membraneAutophagy Protein 5Human medicineMESH: Biological Assay/methods*Neuroscienceautolysosome; autophagosome; flux; LC3; lysosome; phagophore; stress; vacuoleAutophagy
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A frozen analogue approach to aminopyridinylimidazoles leading to novel and promising p38 MAP kinase inhibitors.

2012

In this study we report the design, synthesis, and biological evaluation of constrained aminopyridinylimidazoles as p38α MAP kinase inhibitors. The frozen analogue approach focused on the pyridinyl unit, using purine bioisosteres as constrained structure analogues. The identification of the most potent bioisostere was followed by a further derivatization to address hydrophobic region II. In combination with C-2 modifications of the imidazole core, we were able to design highly active inhibitors on the p38α MAP kinase. The inhibitor design presented herein represents a promising and highly efficient advancement of recent stages of development in this class of p38 MAP kinase inhibitors. In co…

biologyChemistryStereochemistryPyridinesp38 mitogen-activated protein kinasesEntropyImidazolesMolecular ConformationCombinatorial chemistryp38 Mitogen-Activated Protein KinasesMolecular conformationMolecular Docking Simulationchemistry.chemical_compoundStructure-Activity RelationshipPurinesMitogen-activated protein kinaseDrug DesignDrug Discoverybiology.proteinMolecular MedicineStructure–activity relationshipBioisostereBiological evaluationJournal of medicinal chemistry
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Hsp56 protein and mRNA distribution in normal and stressed P. lividus embryos

2008

It was previously demonstrated that Paracentrotus lividus Hsp56 mitochondrial chaperonin is con- stitutively expressed during development, that it increases after heat-shock and cadmium treatment, and that it has a specific territorial distribution, both in normal and heat-shocked embryos, as shown by immunolocalization experiments. In this work, we analyzed by Western blot the territorial distribution of the protein in plutei exposed to heat-shock or sublethal cadmium concentrations, and we found that Hsp56 increases in both ectodermal and en- dodermal cells. Moreover, by “in situ” hybridization, we looked at Hsp56 mRNA during normal development and under stress conditions. We found that th…

cadmium chaperonin mitochondria
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Effect of cadmium on mitochondria-related activity and gene expression in tumoral and immortalized human breast cells

2007

cadmium mitochondria cancer cells
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Effetto del cadmio sull'attività mitocondriale e sull'espressione genica in cellule tumorali e immortalizzatedi epitelio ghiandolare mammario

2007

cadmium mitochondria tumor cells
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Dissecting the Mechanism of Action of Spiperone—A Candidate for Drug Repurposing for Colorectal Cancer

2022

Approximately 50% of colorectal cancer (CRC) patients still die from recurrence and metastatic disease, highlighting the need for novel therapeutic strategies. Drug repurposing is attracting increasing attention because, compared to traditional de novo drug discovery processes, it may reduce drug development periods and costs. Epidemiological and preclinical evidence support the antitumor activity of antipsychotic drugs. Herein, we dissect the mechanism of action of the typical antipsychotic spiperone in CRC. Spiperone can reduce the clonogenic potential of stem-like CRC cells (CRC-SCs) and induce cell cycle arrest and apoptosis, in both differentiated and CRC-SCs, at clinically relevant co…

cancer stem cellsCancer ResearchrepurposingNeoplasms. Tumors. Oncology. Including cancer and carcinogenscolorectal cancerpsychotropic drugsrepurposing; phospholipase C; colorectal cancer; endoplasmic reticulum stress; intracellular calcium; lipid metabolism; psychotropic drugs; cancer stem cells; mitochondria; GolgimitochondriaOncologylipid metabolism&nbspGolgiendoplasmic reticulum stressSettore MED/46 - Scienze Tecniche Di Medicina Di Laboratoriophospholipase CRC254-282intracellular calciumCancers
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In vitro effects of extracts from leaves and rhizomes of P.oceanica on HepG2 tumor cells

2023

cell biology tumor cells posidonia oceanica anticancer cytotoxic apoptosis autophagy cell viability mitochondria cell cycle redox state wound healing assaySettore CHIM/10 - Chimica Degli AlimentiSettore BIO/05 - ZoologiaSettore BIO/06 - Anatomia Comparata E Citologia
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