Search results for " Modification"
showing 10 items of 781 documents
Protein S-nitrosylation: specificity and identification strategies in plants
2015
SPE Pôle IPM UB; International audience; The role of nitric oxide (NO) as a major regulator of plant physiological functions has become increasingly evident. To further improve our understanding of its role, within the last few years plant biologists have begun to embrace the exciting opportunity of investigating protein S-nitrosylation, a major reversible NO-dependent post-translational modification (PTM) targeting specific Cys residues and widely studied in animals. Thanks to the development of dedicated proteomic approaches, in particular the use of the biotin switch technique (BST) combined with mass spectrometry, hundreds of plant protein candidates for S-nitrosylation have been identi…
Post-translational Modifications in Plant Nuclear Signaling: Novel Insights Into Responses to Environmental Changes
2019
Just imagine a Plant Science professor in front of a classroom full of interested and attentive students. Imagine what their answers to this intriguing question would be: “What are, according to you, the functions ensured by the plant cell nucleus?” It would be very surprising if some of them would answer cell signaling in response to biotic and abiotic stresses or developmental processes. Most of them would probably answer according to a classical point of view: DNA replication or gene expression. Hence it is still admitted in recent publications (see for instance Fedorenko et al., 2010) that molecules smaller than 40 kDa can diffuse freely across the nuclear envelope pores. However, Pauly…
Nanovector formation by functionalization of TRAIL ligand on single-walled carbon nanotube: Experimental and theoretical evidences
2015
Équipe 104 : Nanomatériaux; International audience; The synthesis and the characterization of a novel nanovector based on oxidized single-walled carbon nanotubes (SWCNT) functionalized with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via noncovalent 1-pyrenebutanoic acid N-hydrosuccinimid ester (PSE) is described. Experimental noncovalent functionalized SWCNT by PSE are compared to full DFT theoretical predictions. For this, several experimental techniques are gathered to prove the well functionalization of oxidized SWCNT by pi-pi stacking such as micro Raman and XPS spectroscopy analysis coupled to full-DFT calculations. Scanning transmission electron microscopy (STEM) …
Alcoholic Parotid Sialosis: A Structural and Ultrastructural Study
2004
OBJETIVOS: El propósito del presente trabajo fue establecer posibles diferencias histopatológicas entre los estadíos iniciales y terminales de la sialosis alcohólica, patología que generalmente involucra hipertrofia parotídea y afecta estructuralmente en diverso grado a las demás glándulas salivales. DISEÑO DEL ESTUDIO: se analizaron y compararon las modificaciones estructurales y ultraestructurales de glándulas parótidas provenientes de: A) biopsias de alcohólicos crónicos con diagnóstico clínico de cirrosis hepática, de las que una fracción fue procesada para microscopía óptica (MO) y otra fracción fue fijada en glutaraldehído y procesada para microscopía electrónica de trasmisión (MET) J…
Dipeptides of S-Substituted Dehydrocysteine as Artzyme Building Blocks: Synthesis, Complexing Abilities and Antiproliferative Properties †
2021
Background: Dehydropeptides are analogs of peptides containing at least one conjugate double bond between α,β-carbon atoms. Its presence provides unique structural properties and reaction centre for chemical modification. In this study, the series of new class of dipeptides containing S-substituted dehydrocysteine with variety of heterocyclic moieties was prepared. The compounds were designed as the building blocks for the construction of artificial metalloenzymes (artzymes). Therefore, the complexing properties of representative compounds were also evaluated. Furthermore, the acknowledged biological activity of natural dehydropeptides was the reason to extend the study for antiproliferativ…
An Atom-Economical Approach to Functionalized Single-Walled Carbon Nanotubes: Reaction with Disulfides
2013
Owing to their unique structure, thermal stability, and mechanical and electronic properties, single-walled carbon nanotubes (SWCNTs) have been a subject of continuous and intense interest. However, various applications in many fields, such as molecular electronics, solar cells, and nanomedicine, often require the development of reproducible protocols for the chemical modification of SWCNTs. In fact, one of the main drawbacks of the use of SWCNTs is their tendency to aggregate and intrinsic poor solubility, which prevent their manipulation and limit their potential. To date, several methods have been described for the chemical functionalization of SWCNTs; however, new versatile and reliable…
The Histone Deacetylase Inhibitor ITF2357 (Givinostat) Targets Oncogenic BRAF in Melanoma Cells and Promotes a Switch from Pro-Survival Autophagy to …
2022
Histone deacetylase inhibitors (HDACI) are epigenetic compounds that have been widely considered very promising antitumor agents. Here, we focus on the effects of the pan-HDAC inhibitor ITF2357 (Givinostat) in comparison with SAHA (Vorinostat) in melanoma cells bearing BRAF V600E oncogenic mutation. Our results indicate both ITF2357 and SAHA dose-dependently reduce the viability of BRAF-mutated SK-MEL-28 and A375 melanoma cells. The comparison of IC50 values revealed that ITF2357 was much more effective than SAHA. Interestingly, both inhibitors markedly decreased oncogenic BRAF protein expression levels, ITF2357 being the most effective compound. Moreover, the BRAF decrease induced by ITF23…
Surface Modification of Porous Polyethylene Implants with an Albumin-Based Nanocarrier-Release System
2021
Background: Porous polyethylene (PPE) implants are used for the reconstruction of tissue defects but have a risk of rejection in case of insufficient ingrowth into the host tissue. Various growth factors can promote implant ingrowth, yet a long-term gradient is a prerequisite for the mediation of these effects. As modification of the implant surface with nanocarriers may facilitate a long-term gradient by sustained factor release, implants modified with crosslinked albumin nanocarriers were evaluated in vivo. Methods: Nanocarriers from murine serum albumin (MSA) were prepared by an inverse miniemulsion technique encapsulating either a low- or high-molar mass fluorescent cargo. PPE implants …
A remarkable selectivity in the N-functionalization of polyaza[n]paracyclophanes. Synthesis of N-(4-picolyl)-substituted 2,6,9,13-tetraaza[14]paracyc…
1997
Abstract Interaction of cationic host species, in particular Zn 2+ salts, with polyaza[n]paracyclophanes (i.e. 2,6,9,13-Tetraaza[14]paracyclophane, B323 (1a) ) directs their selective N-functionalization. In this way compounds mono- or difunctionalized with ArCH 2− groups at the benzylic nitrogen atoms can be easily obtained. Direct reaction with the alkylating agent, in the absence of the Zn 2+ species, produces, in general, very complex mixtures of mono- and polyalkylated compounds except when 4-picolyl chloride is used. In this case, mono-, di-, tri- and tetra N-substituted derivatives of D323 can be isolated depending on the amount of the alkylating agent used. In this case, the mono- a…
La religión del cuerpo-mercancía: ¿un nuevo opio del pueblo?
2018
The commercial exploitation or more precisely the total capitalist management of the human body erects this as a universal divinity. The direct consequence of this generalized corporal capitalism is the massive depoliticization of the body and, often, the reflection that is dedicated to it. In this article, we seek to reflect on the current practices of bodily modifications within the framework of the changes in the regimes of neoliberal governance in recent decades. This reflection is guided by the revision of central texts of critical theory