Search results for " Movement"
showing 10 items of 1376 documents
Profilin1 activity in cerebellar granule neurons is required for radial migration in vivo.
2014
Neuron migration defects are an important aspect of human neuropathies. The underlying molecular mechanisms of such migration defects are largely unknown. Actin dynamics has been recognized as an important determinant of neuronal migration, and we recently found that the actin-binding protein profilin1 is relevant for radial migration of cerebellar granule neurons (CGN). As the exploited brain-specific mutants lacked profilin1 in both neurons and glial cells, it remained unknown whether profilin1 activity in CGN is relevant for CGN migration in vivo. To test this, we capitalized on a transgenic mouse line that expresses a tamoxifen-inducible Cre variant in CGN, but no other cerebellar cell …
C1q acts in the tumour microenvironment as a cancer-promoting factor independently of complement activation
2015
Complement C1q is the activator of the classical pathway. However, it is now recognized that C1q can exert functions unrelated to complement activation. Here we show that C1q, but not C4, is expressed in the stroma and vascular endothelium of several human malignant tumours. Compared with wild-type (WT) or C3- or C5-deficient mice, C1q-deficient (C1qa−/−) mice bearing a syngeneic B16 melanoma exhibit a slower tumour growth and prolonged survival. This effect is not attributable to differences in the tumour-infiltrating immune cells. Tumours developing in WT mice display early deposition of C1q, higher vascular density and an increase in the number of lung metastases compared with C1qa−/− mi…
Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming
2018
Summary Mitochondria are key players in the regulation of T cell biology by dynamically responding to cell needs, but how these dynamics integrate in T cells is still poorly understood. We show here that the mitochondrial pro-fission protein Drp1 fosters migration and expansion of developing thymocytes both in vitro and in vivo. In addition, we find that Drp1 sustains in vitro clonal expansion and cMyc-dependent metabolic reprogramming upon activation, also regulating effector T cell numbers in vivo. Migration and extravasation defects are also exhibited in Drp1-deficient mature T cells, unveiling its crucial role in controlling both T cell recirculation in secondary lymphoid organs and acc…
A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology
2015
Bone morphogenetic proteins (BMPs), members of the TGF-β superfamily, have numerous biological activities including control of growth, differentiation, and vascular development. Using an in vitro co-culture endothelial cord formation assay, we investigated the role of a BMP7 variant (BMP7v) in VEGF, bFGF, and tumor-driven angiogenesis. BMP7v treatment led to disruption of neo-endothelial cord formation and regression of existing VEGF and bFGF cords in vitro. Using a series of tumor cell models capable of driving angiogenesis in vitro, BMP7v treatment completely blocked cord formation. Pre-treatment of endothelial cells with BMP7v significantly reduced their cord forming ability, indicating …
Planar Cell Polarity Signaling in Collective Cell Movements During Morphogenesis and Disease
2012
Collective and directed cell movements are crucial for diverse developmental processes in the animal kingdom, but they are also involved in wound repair and disease. During these processes groups of cells are oriented within the tissue plane, which is referred to as planar cell polarity (PCP). This requires a tight regulation that is in part conducted by the PCP pathway. Although this pathway was initially characterized in flies, subsequent studies in vertebrates revealed a set of conserved core factors but also effector molecules and signal modulators, which build the fundamental PCP machinery. The PCP pathway in Drosophila regulates several developmental processes involving collective cel…
Sediment characteristics and macrofauna distribution along a human-modified inlet in the Gulf of Oristano (Sardinia, Italy)
2007
We studied the spatial variability and within-year temporal changes in hydrological features, grain size composition and chemical characteristics of sediments, as well as macrofaunal assemblages, along a heavily modified inlet in the Gulf of Oristano (western Sardinia, Italy). The inlet connects the Cabras lagoon to the gulf through a series of convoluted creeks and man-made structures, including a dam and fish barriers built in the last three decades. Sediments were muddy and mainly composed of the "non-sortable" fraction (i.e., < 8 mu m particle size) in all four areas investigated: Lagoon, Creeks, Channel and Seaward. Along the inlet, however, the ratio between the < 8 mu mn and the 8-64…
A late Eemian aridity pulse in central Europe during the last glacial inception
2005
How do ice ages begin? It's an obvious question to ask as we enjoy the relative luxury of an interglacial, but a hard one to answer. A look at past transitions may give some clues as to how this period will one day come to an end. A climate reconstruction based on sediments found beneath a lake in the Eifel mountains in Germany provides evidence of an extreme climate event lasting 468 years right at the end of the last interglacial. Dust storms, aridity, bushfires and the loss of trees associated with a warm climate coincided with a southward shift of the warm waters of the North Atlantic drift. In terms of insolation — the rate of delivery of the Sun's radiation to Earth — conditions then …
Experimental and numerical investigations on the effect of fracture geometry and fracture aperture distribution on flow and solute transport in natur…
2018
The impact of fracture geometry and aperture distribution on fluid movement and on non-reactive solute transport was investigated experimentally and numerically in single fractures. For this purpose a hydrothermally altered and an unaltered granite drill core with axial fractures were investigated. Using three injection and three extraction locations at top and bottom of the fractured cores, different dipole flow fields were examined. The conservative tracer (Amino-G) breakthrough curves were measured using fluorescence spectroscopy. Based on 3-D digital data obtained by micro-computed tomography 2.5-D numerical models were generated for both fractures by mapping the measured aperture distr…
LONG-TERM STRUCTURAL DEFICIENCIES IN A MAT FOUNDATION ON CLAY SOIL
2013
A 3-story building with a mat foundation consisting of a slab on a grid of grade beams performed poorly on clay soil. Cracking of the slab became rogressively worse as a result of the incorrect esign and fluctuations in the groundwater pressure under the foundation. The cyclic presence and absence of water rusted the steel reinforcement, and the sulfates in the clay soil caused formation of ettringite in the concrete. Plastic hinges formed in the slab and settlements occurred, causing damage to the beams. The situation is still in progress and may lead to the collapse of the structure under normal service conditions. The geotechnical and structural investigations performed to survey and ass…
Ruch pokojowy w RFN (Friedensbewegung) w pierwszej połowie lat osiemdziesiątych XX wieku
2018
In the 1980s, the peace movement, which was mobilizing hundreds of thousands of people in protests against the double act passed by NATO in December 1979, was an integral element of the West-German political stage. It influenced the system of power and internal conflicts within the German Social Democracy (SPD), being the strongest opponent of the policy run by the government of Chancellor Schmidt. It also contributed to the formation of the Green Party and was the object of polemics within trade unions. Apart from that it provided a stimulus for debates of the ethical and religious character within the Catholic and Protestant Churches in the FRG. Lastly, it was the object of controversy an…