Search results for " Oxygen"
showing 10 items of 1626 documents
A review of transgenerational effects of ocean acidification on marine bivalves and their implications for sclerochronology
2020
Abstract Ocean acidification can negatively impact marine bivalves, especially their shell mineralization processes. Consequently, whether marine bivalves can rapidly acclimate and eventually adapt in an acidifying ocean is now increasingly receiving considerable attention. Projecting the fate of this vulnerable taxonomic group is also pivotal for the science of sclerochronology – the study which seeks to deduce records of past environmental changes and organismal life-history traits from various geochemical properties of periodically layered hard tissues (bivalve shells, corals, fish otoliths, etc.). In this review, we provide a concise overview of the long-term and transgenerational respo…
NADPH Oxidase-Mediated Reactive Oxygen Species Production: Subcellular Localization and Reassessment of Its Role in Plant Defense
2009
International audience; Chemiluminescence detection of reactive oxygen species (ROS) triggered in tobacco BY-2 cells by the fungal elicitor cryptogein was previously demonstrated to be abolished in cells transformed with an antisense construct of the plasma membrane NADPH oxidase, NtrbohD. Here, using electron microscopy, it has been confirmed that the first hydrogen peroxide production occurring a few minutes after challenge of tobacco cells with cryptogein is plasma membrane located and NtrbohD mediated. Furthermore, the presence of NtrbohD in detergent-resistant membrane fractions could be associated with the presence of NtrbohD-mediated hydrogen peroxide patches along the plasma membran…
The grapevine flagellin receptor VvFLS2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium Burkholde…
2014
International audience; The role of flagellin perception in the context of plant beneficial bacteria still remains unclear. Here, we characterized the flagellin sensing system flg22-FLAGELLIN SENSING 2 (FLS2) in grapevine, and analyzed the flagellin perception in the interaction with the endophytic plant growth-promoting rhizobacterium (PGPR) Burkholderia phytofirmans. The functionality of the grapevine FLS2 receptor, VvFLS2, was demonstrated by complementation assays in the Arabidopsis thaliana fls2 mutant, which restored flg22-induced H2O2 production and growth inhibition. Using synthetic flg22 peptides from different bacterial origins, we compared recognition specificities between VvFLS2…
Glutathione deficiency of the Arabidopsis mutant pad2-1 affects oxidative stress-related events, defense gene expression and hypersensitive response
2011
L'article original est publié par The American Society of Plant Biologists; International audience; The Arabidopsis (Arabidopsis thaliana) phytoalexin-deficient mutant pad2-1 displays enhanced susceptibility to a broad range of pathogens and herbivorous insects that correlates with deficiencies in the production of camalexin, indole glucosinolates, and salicylic acid (SA). The pad2-1 mutation is localized in the GLUTAMATE-CYSTEINE LIGASE (GCL) gene encoding the first enzyme of glutathione biosynthesis. While pad2-1 glutathione deficiency is not caused by a decrease in GCL transcripts, analysis of GCL protein level revealed that pad2-1 plants contained only 48% of the wild-type protein amoun…
Nitric oxide: comparative synthesis and signaling in animal and plant cells.
2001
Since its identification as an endothelium-derived relaxing factor in the 1980s, nitric oxide has become the source of intensive and exciting research in animals. Nitric oxide is now considered to be a widespread signaling molecule involved in the regulation of an impressive spectrum of mammalian cellular functions. Its diverse effects have been attributed to an ability to chemically react with dioxygen and its redox forms and with specific iron- and thiol-containing proteins. Moreover, the effects of nitric oxide are dependent on the dynamic regulation of its biosynthetic enzyme nitric oxide synthase. Recently, the role of nitric oxide in plants has received much attention. Plants not only…
Quantitative proteomics reveals a dynamic association of proteins to detergent-resistant membranes upon elicitor signaling in tobacco.
2009
International audience; A large body of evidence from the past decade supports the existence, in membrane from animal and yeast cells, of functional microdomains playing important roles in protein sorting, signal transduction, or infection by pathogens. In plants, as previously observed for animal microdomains, detergent-resistant fractions, enriched in sphingolipids and sterols, were isolated from plasma membrane. A characterization of their proteic content revealed their enrichment in proteins involved in signaling and response to biotic and abiotic stress and cell trafficking suggesting that these domains were likely to be involved in such physiological processes. In the present study, w…
Regulation of reactive oxygen species production by a 14-3-3 protein in elicited tobacco cells.
2007
International audience; The regulation of the system responsible for the production of reactive oxygen species (ROS) during plant–microorganism interaction is still largely unknown. The protein NtrbohD has been recently demonstrated as the plasma membrane oxidase responsible for ROS production in elicited tobacco cells. Here, its C-terminus part was used as a bait in a two-hybrid screen in order to identify putative regulators of this system. This led to the isolation of a cDNA coding for a member of the 14-3-3 protein family. The corresponding transcript was induced after infiltration of tobacco leaves with the fungal elicitor cryptogein. Tobacco cells transformed with an antisense constru…
Glycymeris pilosa (Bivalvia) - A high-potential geochemical archive of the environmental variability in the Adriatic Sea.
2019
Due to its outstanding longevity (decades), the shallow-water bivalve Glycmeris pilosa represents a prime target for sclerochronological research in the Mediterranean Sea. In the present study, we analyzed the microgrowth patterns and the stable carbon (δ13Cshell) and oxygen (δ18Oshell) isotopes of the outer shell layer of live-collected G. pilosa specimens from four different sites along the Croatian coast, middle Adriatic Sea. Combined analysis of shell growth patterns and temporally aligned δ18Oshell data indicated that the main growing season lasts from April to December, with fastest growth rates occurring during July and August when seawater temperatures exceeded 22 °C. Slow growth in…
Chlorophyll fluorescence, photosynthesis and growth of tomato plants as affected by long-term oxygen root zone deprivation and grafting
2020
A greenhouse experiment was conducted to study the effects of the O2 root zone level and grafting on chlorophyll fluorescence, photosynthesis and growth of cherry tomato grown in a hydroponic system. Two O2 concentrations in the root zone, namely Ox (saturation level) and Ox- (2&ndash
The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells
2002
Summary A cDNA encoding a protein, NtrbohD, located on the plasma membrane and homologue to the flavocytochrome of the neutrophil NADPH oxidase, was cloned in tobacco. The corresponding mRNA was accumulated when tobacco leaves and cells were treated with the fungal elicitor cryptogein. After elicitation with cryptogein, tobacco cells transformed with antisense constructs of NtrbohD showed the same extracellular alkalinization as the control, but no longer produced active oxygen species (AOS). This work represents the first demonstration of the function of a homologue of gp91–phox in AOS production in elicited tobacco cells.