Search results for " PC"

showing 10 items of 434 documents

A polyphasic approach to the identification of ochratoxin A-producing black Aspergillus isolates from vineyards in Sicily.

2008

Aspergillus strains belonging to section Nigri isolated during a two year survey in eight Sicilian vineyards located on the slopes of Mount Etna (Sicily, Italy) were analysed analyzed in order to characterize species responsible for ochratoxin A (OTA) contamination of grapes. The polyphasic approach permitted analysis of biodiversity of Aspergillus isolates in relation to their morphology, ochratoxigenicity and genetic variability. We assessed OTA production by A. carbonarius, A. niger, A. tubingensis and A. japonicus using an enzyme-linked immunosorbent assay. A. carbonarius isolates were the strongest OTA producers. A subset of 66 representative strains was selected for further DNA-based …

Ochratoxin AVeterinary medicineEnzyme-Linked Immunosorbent AssayFood ContaminationfAFLPMicrobiologylaw.inventionMicrobiologychemistry.chemical_compoundVineyard OTA A. carbonariusPCR identificationSpecies SpecificitylawDNA Ribosomal SpacerCluster AnalysisVitisGenetic variabilityDNA FungalOchratoxinAspergillus; Ochratoxin A; fAFLP; PCR identification; 16s rDNA sequencingPolymerase chain reactionPhylogenyAspergillusbiology16s rDNA sequencingAspergillus nigerOchratoxin AGeneral MedicineFungi imperfectiSequence Analysis DNAbiology.organism_classificationOchratoxinsRNA Ribosomal 5.8SAspergilluschemistryItalyAspergillus nigerRestriction fragment length polymorphismPolymorphism Restriction Fragment LengthFood ScienceInternational journal of food microbiology
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The Detection of Androgen Receptor Splice Variant 7 in Plasma-derived Exosomal RNA Strongly Predicts Resistance to Hormonal Therapy in Metastatic Pro…

2017

Abstract Background The androgen receptor splice variant 7 (AR-V7) is associated with resistance to hormonal therapy in castration-resistant prostate cancer (CRPC). Due to limitations of the methods available for AR-V7 analysis, the identification of a reliable detection method may facilitate the use of this biomarker in clinical practice. Objective To confirm AR-V7 as a predictor of resistance to hormonal therapy and develop a new approach to assess AR-V7 by highly sensitive digital droplet polymerase chain reaction (ddPCR) in plasma-derived exosomal RNA. Design, setting, and participants Plasma samples were collected from 36 CRPC patients before they began second-line hormonal treatment. …

Oncology0301 basic medicineMaleResistanceExosomeschemistry.chemical_compoundProstate cancer0302 clinical medicineProtein IsoformsNeoplasm MetastasisReceptorAged 80 and overProstate cancerMiddle AgedProstatic Neoplasms Castration-ResistantReceptors Androgen030220 oncology & carcinogenesisBenzamidesAdenocarcinomaBiomarker (medicine)Hormonal therapyAR-V7; Digital droplet PCR; Exosomes; Hormonal therapy; Pharmacogenetics; Prostate cancer; Resistance; UrologyAndrostenesHormonal therapymedicine.medical_specialtyAntineoplastic Agents Hormonalmedicine.drug_classUrologyCastration resistantAdenocarcinomaDisease-Free Survival03 medical and health sciencesSDG 3 - Good Health and Well-beingInternal medicineNitrilesPhenylthiohydantoinmedicineEnzalutamideHumansAgedDigital droplet PCRPlasma derivedbusiness.industryRNAAndrogen Receptor Splice Variant 7medicine.diseaseAndrogenEndocrinology030104 developmental biologychemistryPharmacogeneticsDrug Resistance NeoplasmCancer cellCancer researchRNAAR-V7businessPharmacogenetics
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The experience of the International Consortium on Acute Promyelocytic Leukemia in monitoring minimal residual disease in acute promyelocytic leukaemia

2016

OncologyAcute promyelocytic leukemiaacute leukaemiamedicine.medical_specialtyacute promyelocytic leukaemiaPROTEÍNAS PROTO-ONCOGÊNICAS03 medical and health sciences0302 clinical medicineInternal medicinemedicineNeoplasmSurvival ratebusiness.industryFollow up studiesHematologymedicine.diseaseMinimal residual diseaseClinical trialLeukemiaPML/RARA; acute leukaemia; acute promyelocytic leukaemia; minimal residual disease; quantitative PCR030220 oncology & carcinogenesisquantitative PCRminimal residual diseaseAcute promyelocytic leukaemiabusinessPML/RARASettore MED/15 - Malattie del Sangue030215 immunology
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Evaluation of a combined triple method to detect causative HPV in oral and oropharyngeal squamous cell carcinomas: p16 Immunohistochemistry, Consensu…

2012

Abstract Background Recent emerging evidences identify Human Papillomavirus (HPV) related Head and Neck squamous cell carcinomas (HN-SCCs) as a separate subgroup among Head and Neck Cancers with different epidemiology, histopathological characteristics, therapeutic response to chemo-radiation treatment and clinical outcome. However, there is not a worldwide consensus on the methods to be used in clinical practice. The endpoint of this study was to demonstrate the reliability of a triple method which combines evaluation of: 1. p16 protein expression by immunohistochemistry (p16-IHC); 2. HPV-DNA genotyping by consensus HPV-DNA PCR methods (Consensus PCR); and 3 viral integration into the host…

OncologyHuman papillomavirusHPVCancer Researchmedicine.medical_specialtyPathologyMethylation-Specific PCREpidemiologySettore MED/50 - Scienze Tecniche Mediche ApplicateBisulfite sequencingConsensus PCRIn situ hybridizationSettore MED/08 - Anatomia Patologicalcsh:RC254-282lcsh:Infectious and parasitic diseasesHPV-DNA; in situ hybridationSettore MED/28 - Malattie OdontostomatologicheInternal medicinemedicineDNA consensus PCRlcsh:RC109-216p16-IHCin situ hybridationGenotypingHN-SCCOPSCCbusiness.industryHead and neck cancerEpigeneticHPV-DNAHead and neck squamous cell carcinomalcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogensmedicine.diseaseImmunohistochemistryHead and neck squamous-cell carcinomastomatognathic diseasesInfectious DiseasesReal-time polymerase chain reactionOncologyImmunohistochemistryOSCCbusinessResearch ArticleIHCInfectious Agents and Cancer
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Prophylactic cranial irradiation in stage IV small cell lung cancer

2019

Background: Due to conflicting results between major trials the role of prophylactic cranial irradiation (PCI) in stage IV small cell lung cancer (SCLC) is controversial.Methods: We obtained a list of 13 European experts from both the European Society for Therapeutic Radiation Oncology (ESTRO) and the International Association for the Study of Lung Cancer (IASLC). The strategies in decision making for PCI in stage IV SCLC were collected. Decision trees were created representing these strategies. Analysis of consensus was performed with the objective consensus methodology.Results: The factors associated with the recommendation for the use of PCI included the fitness of the patient, young age…

OncologyMaleESTRO; Expert opinion; IASLC; PCI; Small cell lung cancer; Stage IV; Hematology; Oncology; Radiology Nuclear Medicine and ImagingLung Neoplasmsmedicine.medical_treatmentMedizin030218 nuclear medicine & medical imaging0302 clinical medicineQUALITY-OF-LIFENuclear Medicine and ImagingESTRO610 Medicine & healthStage IVHematologyBrain NeoplasmsRadiation OncologistsPCIHematologyMiddle AgedProgression-Free SurvivalExpert opinion030220 oncology & carcinogenesisFemaleTRIALNon small cellRadiologyAdultmedicine.medical_specialtyDecision Making03 medical and health sciencesInternal medicinemedicineHumansIASLCRadiology Nuclear Medicine and imagingLung cancerAgedNeoplasm StagingChemotherapySmall cell lung cancerbusiness.industryPatient Selectionmedicine.diseaseONCOLOGYSmall Cell Lung CarcinomaYoung ageConventional PCIProphylactic cranial irradiationCranial IrradiationbusinessStage ivRadiotherapy and Oncology
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0112: Major improvement in one-year mortality in elderly patients with acute myocardial infarction in relation with early PCI and recommended medicat…

2016

Background and aimOutcomes of AMI patients have substantially improved over the past 2 decades. Whether similar trends are observed in elderly (≥75 years of age) and younger patients has not been extensively studied.MethodsWe analysed one-year mortality of elderly vs younger patients in 4 nationwide French survey carried out 5 years apart from 1995 to 2010. Consecutive STEMI and NSTEMI patients (≤48 hours from onset) were recruited over one-month periods. Among 10610 patients included in the 4 surveys, 3389 (32%) were aged 75+.ResultsFrom 1995 to 2010, the proportion of 75+remained stable in NSTEMI (1995: 36%, 2010: 38%), but decreased in STEMI patients (1995: 30%, 2010: 25.5%, P=0.006). Us…

One year mortalitySecondary preventionPediatricsmedicine.medical_specialtyAge groupsbusiness.industryConventional PCIMedicineMyocardial infarctionCardiology and Cardiovascular Medicinebusinessmedicine.diseaseStrokeArchives of Cardiovascular Diseases Supplements
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Si photomultipliers for bio-sensing applications

2016

In this paper, silicon photomultipliers (SiPM) are proposed as optical detectors for bio sensing. Optical transduction is the most used detection mechanism in many biosensor applications, such as DNA microarray and real-time polymerase chain reaction. The performances of a 25 pixels device used for both applications are studied. The results confirm that the SiPM is more sensitive than the traditionally employed detectors. In fact, it is able to experimentally detect 1 nM and 100 fM of fluorophore concentrations in dried samples and solutions, respectively. We present and discuss in details the detector configuration and its characterization as fluorescence detector for bio sensing.

Optical biosensor systemPhotomultiplierFluorophoreMaterials sciencePixel010308 nuclear & particles physics010401 analytical chemistryDetectorDNA microarrayDNA microarray Optical biosensor system RT PCR Silicon PhotomultipliersNanotechnologySilicon Photomultipliers01 natural sciencesFluorescenceSettore ING-INF/01 - ElettronicaAtomic and Molecular Physics and OpticsFluorescence spectroscopy0104 chemical scienceschemistry.chemical_compoundSilicon photomultiplierchemistry0103 physical sciencesElectrical and Electronic EngineeringBiosensorRT PCR
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A PCI Express optical link based on low-cost transceivers qualified for radiation hardness

2013

In this paper we want to demonstrate that an optical physical medium is compatible with the second generation of PCI Express. The benefit introduced by the optical decoupling of a PCI Express endpoint is twofold: it allows for a geographical detachment of the device and it remains compliant with the usual PCI accesses to the legacy I/O and memory spaces. We propose two boards that can bridge the PCI Express protocol over optical fiber. The first is a simple optical translator while the second is a more robust switch developed for connecting up to four devices to a single host. Such adapters are already working in the control and data acquisition system of a particle detector at CERN and hen…

Optical fiberbusiness.industryComputer scienceM.2Optical linkElectrical engineeringlaw.inventionData acquisitionlawConventional PCITransceiverbusinessInstrumentationRadiation hardeningMathematical PhysicsComputer hardwarePCI ExpressJournal of Instrumentation
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Improved performance in flexible organic solar cells by using copolymeric phase-separation modulators

2018

One of the main problems related to the low performance of the organic solar cells (OSCs), concerns the low mobility of the materials constituting the heterojunction. Indeed, the poor charge transport in the active layer is the principal cause of a competition between separation and recombination of the photogenerated carriers. In this regard, a major obstacle to enhance OSCs efficiency is developing strategies to optimize the exciton dissociation and, consequently, the charge collection at the electrodes. Donor and acceptor systems must be well mixed on the length scale of 5 – 20 nm (exciton diffusion length) to meet the criteria for efficient exciton dissociation. In addition, the network…

Organic solar cells flexible devices modulators phase segregation copolymer fullerene PCBM P3HT
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Caratterizzazione chimico-morfologica di accessioni spontanee di origano siciliano (Origanum heracleoticum L.) del territorio dei monti Nebrodi

2009

Il genere Origano è estremamente diffuso nel bacino del Mediterraneo, con diverse specie presenti nel territorio siciliano. Il comprensorio dei Monti Nebrodi (Sicilia N-E), esteso per circa 200.000 ettari, è una delle aree siciliane in cui è presente la massima biodiversità vegetale; l'area è caratterizzato prevalentemente dalla presenza di Origanum heracleoticum, reperibile in ambienti ecologicamente anche assai diversificati. Con l'obiettivo di acquisire informazioni utili ai fini della caratterizzazione delle accessioni di Origanum heracleoticum spontanee nel comprensorio nebroideo, a partire dal 2007 è stato avviato su di esse un lavoro di ricognizione e collezione sistematica. Campioni…

Origano Origanum heracleoticum L. cluster analysis.Origano Origanum heracleoticum L. cluster analysis PCASettore AGR/02 - Agronomia E Coltivazioni Erbacee
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