6533b7ddfe1ef96bd127494d

RESEARCH PRODUCT

Si photomultipliers for bio-sensing applications

Maria Francesca SantangeloMaria Francesca SantangeloEmanuele Luigi SciutoSalvatore A. LombardoAlessandro C. BusaccaSalvatore PetraliaSabrina ConociSebania Libertino

subject

Optical biosensor systemPhotomultiplierFluorophoreMaterials sciencePixel010308 nuclear & particles physics010401 analytical chemistryDetectorDNA microarrayDNA microarray Optical biosensor system RT PCR Silicon PhotomultipliersNanotechnologySilicon Photomultipliers01 natural sciencesFluorescenceSettore ING-INF/01 - ElettronicaAtomic and Molecular Physics and OpticsFluorescence spectroscopy0104 chemical scienceschemistry.chemical_compoundSilicon photomultiplierchemistry0103 physical sciencesElectrical and Electronic EngineeringBiosensorRT PCR

description

In this paper, silicon photomultipliers (SiPM) are proposed as optical detectors for bio sensing. Optical transduction is the most used detection mechanism in many biosensor applications, such as DNA microarray and real-time polymerase chain reaction. The performances of a 25 pixels device used for both applications are studied. The results confirm that the SiPM is more sensitive than the traditionally employed detectors. In fact, it is able to experimentally detect 1 nM and 100 fM of fluorophore concentrations in dried samples and solutions, respectively. We present and discuss in details the detector configuration and its characterization as fluorescence detector for bio sensing.

yearjournalcountryeditionlanguage
2016-05-01
10.1109/jstqe.2015.2504979http://hdl.handle.net/10447/351806