Search results for " PCR"
showing 10 items of 153 documents
Mapping field-scale spatial distribution patterns of size and activity of the denitrifier community
2008
International audience
Développement et évaluation d'une méthode fondée sur la PCR temps réel pour la caractérisation des bioaérosols : application au groupe des actinomycè…
2013
Actinomycetes are ubiquitous bacteria and some can be potentially pathogen for Humans in the air of some working areas. It’s notably the case in composting plants where bacteria concentrations can reach high values. Workers exposure to these inhalable bioaerosols can be source of various diseases (hypersensitivity pneumonitis notably). Although this problem is admitted, bibliography reveals a lack of knowledge about risk assessment: currently, none global method for bioaerosols sampling and analysis is standardized. So much that neither dose-effects relationship for most of these bacteria, nor Threshold Limit Value exists. Traditional methods, that are used, have some drawbacks (concentrati…
Duplex PCR method for rapid detection of Zymomonas mobilis in cider
2005
Quantification of denitrifiers by real-time PCR
2007
Technosols to reclaim industrial wastelands: depth distribution of abundance and activity of N-cycling microbial communities
2012
International audience; Construction of Technosols through assemblage of treated soil and recycled wastes is an innovative option for the restoration of degraded lands and re-use of industrial wastes. Recent studies have evidenced that Technosols could support soil functions such as primary production but the knowledge about other ecosystemic services, such as nutrient cycling, is limited. In this work, we investigated the abundance and the activity of microbial communities involved in N-cycling in different horizons (0–15, 15–35, 35–70 cm) of two types of Technosols constructed to reclaim an industrial wasteland. The estimation by real-time PCR of the abundances of the different microbial …
Species-specific prey choice of carabid beetles in European cereal fields
2017
National audience; Trophic interactions between species in agroecosystems provide key regulation ecosystem services and therefore also determine the dynamics, robustness and resilience of service provision. To achieve international goals of reducing application of pesticides without compromising key provisioning ecosystem services such as crop yield, recent research attaches importance to the biological control potential of carabid beetles. However, apart from feeding on pest species and weed seeds, carabids also consume non-pest prey (alternative prey) such as collembolans and earthworms, which can play a contradictory role in the efficacy of pest and weed control. Most carabids are descri…
Améliorer la méthodologie standard de la qPCR pour l’analyse de la densité microbienne dans la plateforme GenoSol. Mémoire de stage BTS de 2ème année…
2019
Le stage a été dédié à la comparaison de la méthode de ddPCR à celle de la PCR quantitative pour évaluer le nombre de copies de gènes 16S dans un échantillon d’ADN extrait de sol.
Studies of root colonization by a microcosm community of arbuscular mycorrhizal fungi unsing nested PCR
1998
International audience
Application of novel polyphasic approach to study the lactobacilli composition of sourdoughs from the Abruzzo region (Central Italy)
2006
Role of nitric oxide synthase (NOS) of Klebsormidium nitens: Identification and characterization of partners
2021
National audience; Nitric oxide (NO) is an important cellular signaling molecule across kingdoms. During bioticor abiotic stresses, NO burst is detected in both plants and mammals although no sequenceencoding the well described mammalian NO synthase (NOS) is highlighted in plants. Comparedto terrestrial plants, some algae present transcripts encoding the NOS-like enzyme. Amongthem, Klebsormidium nitens the model alga to study the early transition step from aquatic algaeto land plants is found. As mechanisms governing NO synthesis and signaling in green lineageremain unclear, the study of NOS from K. nitens (KnNOS) through (i) the identification ofregulator proteins, (ii) the identification …