Search results for " Positive"

General Principles for the Detection of Modified Nucleotides in RNA by Specific Reagents.

2021

Epitranscriptomics heavily rely on chemical reagents for the detection, quantification, and localization of modified nucleotides in transcriptomes. Recent years have seen a surge in mapping methods that use innovative and rediscovered organic chemistry in high throughput approaches. While this has brought about a leap of progress in this young field, it has also become clear that the different chemistries feature variegated specificity and selectivity. The associated error rates, e.g., in terms of false positives and false negatives, are in large part inherent to the chemistry employed. This means that even assuming technically perfect execution, the interpretation of mapping results issuin…

Qualitat del servei i sabotatge dels empleats: relacions amb la satisfacció i les intencions de comportament dels clients

2019

espanolEn el presente estudio se analizan las relaciones de la calidad de servicio y de los comportamientos de sabotaje de los empleados hacia los clientes, con la satisfaccion y las intenciones de comportamiento de 90 clientes de una organizacion de servicios de la Comunidad Valenciana. Los resultados indican la existencia de diferentes relaciones entre las dos dimensiones de calidad de servicio (funcional y relacional) y los comportamientos de sabotaje de los empleados, por un lado, y las respuestas de los usuarios (satisfaccion, intenciones positivas hacia la organizacion e intencion negativa de advertir a otros clientes sobre la mala calidad del servicio), por otra. Finalmente se reflex…

Interest of exome sequencing trio-like strategy based on pooled parental DNA for diagnosis and translational research in rare diseases.

2021

Abstract Background Exome sequencing (ES) has become the most powerful and cost‐effective molecular tool for deciphering rare diseases with a diagnostic yield approaching 30%–40% in solo‐ES and 50% in trio‐ES. We applied an innovative parental DNA pooling method to reduce the parental sequencing cost while maintaining the diagnostic yield of trio‐ES. Methods We pooled six (Agilent‐CRE‐v2–100X) or five parental DNA (TWIST‐HCE–70X) aiming to detect allelic balance around 8–10% for heterozygous status. The strategies were applied as second‐tier (74 individuals after negative solo‐ES) and first‐tier approaches (324 individuals without previous ES). Results The allelic balance of parental‐pool v…

HLA antigens in Sicilian patients affected by chronic myelogenous leukaemia.

1987

SUMMARY HLA antigens were investigated in Sicilian patients with chronic myelogenous leukaemia (CML) and in Sicilian healthy controls. The frequency of the HLA-DRw6 antigen was significantly decreased in the group of patients. These results suggest that DRw6 may be a marker for decreased susceptibility to the etiological or pathogenic mechanism(s) which produce CMLs.

Detection of mammalian carcinogens with an immunological DNA synthesis-inhibition test.

1992

There is a close relationship between genotoxicity, mutagenicity and carcinogenicity. But the controversy of which short-term test system best recognizes human carcinogens is still going on. Currently, the Salmonella gene mutation assay ('Ames test') is the most widely used test for the screening of mutagens. However, many in vitro tests hold unsatisfactory validity data, presumably because of the inability of present short-term tests to detect non-genotoxic carcinogens, which are increasingly being brought into focus in the discussions of genesis of cancer. One principle often neglected in this context is the property of genotoxic agents to inhibit replicative DNA synthesis in (proliferati…

Environmental Sustainability Approaches and Positive Energy Districts: A Literature Review

2021

During the last decade, increasing attention has been paid to the emerging concept of Positive Energy Districts (PED) with the aim of pushing the transition to clean energy, but further research efforts are needed to identify design approaches optimized from the point of view of sustainable development. In this context, this literature review is placed, with a specific focus on environmental sustainability within innovative and eco-sustainable districts. The findings show that some sustainability aspects such as sustainable food, urban heat islands mitigation and co-impacts, e.g., green gentrification, are not adequately assessed, while fragmented thinking limits the potential of circularit…

Food Processing and Foodborne Illness

2015

Food processing means manipulation of raw materials from plants or animals into food. Since agriculture and animal husbandry were established postharvest procedures were necessary to preserve for a longer time the increasing food supplies coming from the fields and domesticated animals. Food is one of the best culture medium for a lot of microorganisms and preventing food spoilage caused by enzymes and microorganisms is crucial. Deterioration of food is caused mainly by three natural processes: 1. Oxidation – vegetables after harvesting or meat after animal slaughtering get in contact with the oxygen in the air that makes them rancid and unpleasant. 2. Enzyme action – all food contains natu…

Psychometric Comparisons of Benevolent and Corrective Humor across 22 Countries: The Virtue Gap in Humor Goes International

2018

Recently, two instances of virtue-related humor: benevolent and corrective, have been introduced. Benevolent humor treats human weaknesses and wrongdoings benevolently, while corrective humor aims at correcting and bettering them. Twelve marker items for benevolent and corrective humor (BenCor) were developed, and it was demonstrated that they fill the gap between humor as temperament and virtue. The present study investigates responses to the BenCor from 25 samples in 22 countries (overall N = 7, 226). The psychometric properties of the BenCor were found to be sufficient in most of the samples, including internal consistency, unidimensionality, and factorial validity. Importantly, benevole…

Interferon-α for HBeAg-positive chronic hepatitis B

2004

Klinische Bedeutung des Hepatitis-B-Virus-DNS-Nachweises im Serum von Kindern mit chronischer Hepatitis B

1992

206 sera from 172 children with chronic hepatitis B infection were tested for HBV DNA by dot blot hybridization. 111 were positive and 95 negative for HBV DNA. 103 (78.6%) of the positive patients had HBeAg and 5 (7.7%) anti-HBe. In 60 (92.3%) of the anti-HBe positive sera no HBV DNA could be detected. Children with elevated liver enzymes had HBV DNA in 80.1%, whereas in 71.6% of the chronic HBsAg carriers with normal liver enzymes no HBV DNA was found. In 87 of the 95 dot blot negative patients polymerase chain reaction was performed. 73 (83.9%) children of this group were HBV DNA positive. All HBeAg positive patients and those with elevated aminotransferases had HBV DNA in their serum. 56…