Search results for " Pseudomonas aeruginosa"
showing 10 items of 21 documents
Nanometric ion pair complexes of tobramycin forming microparticles for the treatment of Pseudomonas aeruginosa infections in cystic fibrosis
2019
Abstract Sustained pulmonary delivery of tobramycin from microparticles composed of drug/polymer nanocomplexes offers several advantages against traditional delivery methods. Namely, in patients with cystic fibrosis, microparticle delivery can protect the tobramycin being delivered from strong mucoadhesive interactions, thus avoiding effects on its diffusion toward the infection site. Polymeric ion-pair complexes were obtained starting from two synthetic polyanions, through impregnation of their solid dissociated forms with tobramycin in aqueous solution. The structure of these polymeric systems was characterized, and their activities were examined against various biofilm-forming Pseudomona…
Paracentrin1, a synthetic antimicrobial peptide fragment of a beta-thymosin from the sea-urchin Paracentrotus lividus, interferes whith staphylococca…
2014
ATTIVITÀ ANTIBATTERICA DI UNA SOLUZIONE OLEOSA: possibili applicazioni nell’industria alimentare per la prevenzione della formazione di biofilm
2014
Le malattie che derivano dal consumo di alimenti hanno sempre minacciato la salute umana. Oggi il loro aumento è diventato un problema per salute pubblica, emergente in tutto il mondo. Molte di queste malattie sono state associate alla presenza di biofilm batterici, comunità microbiche complesse, nell’industria alimentare. Il biofilm è diventato un grosso problema per l’industria della lavorazione del pesce, del latte e derivati, del pollame, della carne e dei cibi “pronti da mangiare”, perché, in seguito alla sua formazione, i batteri a esso associati sono particolarmente resistenti agli agenti antimicrobici e alle operazioni di pulizia. Anche se fino ad oggi sono ancora utilizzate, e sono…
Uso del tonometro e rischio infettivo ; valutazione dell'efficacia di differenziali tipi di disinfettanti.
2006
Obiettivo: valutare e confrontare l’efficacia dei più comuni tipi di disinfettanti per la disinfezione dei coni tonometrici e trarre alcune indicazioni sulla applicabilità alla realtà clinico-diagnostica in campo oftalmologico. Materiali e metodi: coni tonometrici monouso sterili, previamente contaminati con ceppi standard di Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa e Candida albicans sono stati testati, dopo semplice detersione o disinfezione con acqua ossigenata al 3%, benzalconio cloruro allo 0.5%, Minuten wipes (Alpro® ), Pantasept® allo 0.5% secondo le linee guida del CDC di Atlanta. Risultati: la semplice detersione è stata inefficace contro tutte le specie tes…
A multifunctional peptidomimetic macromolecule to fight polymicrobial infections
2018
Curcumin derivatives as inhibitors of Gram positive and Gram negative biofilm formation
2015
Nano into Micro Formulations of Tobramycin for the Treatment of Pseudomonas aeruginosa Infections in Cystic Fibrosis.
2017
Here, nano into micro formulations (NiMs) of tobramycin for the treatment of Pseudomonas aeruginosa airway infections in cystic fibrosis (CF) are described. NiMs were produced by spray drying a solution containing polymers or sugars and a nanometric polyanion–tobramcyin complex (PTC), able to achieve a prolonged antibiotic release. NiMs properties were compared to TOBIPodhaler(Novartis), the only one commercially available dry powder inhalatory formulation based on porous microparticles. Produced NiMs showed adequate characteristics for pulmonary administration, as spherical shape, micrometric size, and high cytocompatibility toward human bronchial epithelial cells. Contrarily to TOBIPodhal…
Developing Antibiofilm Fibrillar Scaffold with Intrinsic Capacity to Produce Silver Nanoparticles
2022
The development of biomedical systems with antimicrobial and antibiofilm properties is a difficult medical task for preventing bacterial adhesion and growth on implanted devices. In this work, a fibrillar scaffold was produced by electrospinning a polymeric organic dispersion of polylactic acid (PLA) and poly(α,β-(N-(3,4-dihydroxyphenethyl)-L-aspartamide-co-α,β-N-(2-hydroxyethyl)-L-aspartamide) (PDAEA). The pendant catechol groups of PDAEA were used to reduce silver ions in situ and produce silver nanoparticles onto the surface of the electrospun fibers through a simple and reproducible procedure. The morphological and physicochemical characterization of the obtained s…
Risk of Bacillus cereus and Pseudomonas aeruginosa nosocomial infections in a Burns Centre: the microbiological monitoring of water supplies for a pr…
1995
This paper reports on an extensive epidemiological survey of the microbiological monitoring of the environment, staff, and patients in the Intensive Care Unit of the Palermo Bums Centre (Italy). The aim of the survey was to evaluate the presence and distribution of environmental sources of pathogens and opportunistic bacterial agents of nosocomial infection in immunocompromised hosts. Strains collected from air, tap water, and medical and nursing staff were compared with strains isolated from burn patients in order to study the potential transmission route of bacteria. The results showed environmental strains presenting a profile identical to that of the clinical strains, suggesting a link …
Immunoaffinity purification and characterization of mitochondrial membrane-bound D-3-hydroxybutyrate dehydrogenase from Jaculus orientalis.
2008
Abstract Background The interconversion of two important energy metabolites, 3-hydroxybutyrate and acetoacetate (the major ketone bodies), is catalyzed by D-3-hydroxybutyrate dehydrogenase (BDH1: EC 1.1.1.30), a NAD+-dependent enzyme. The eukaryotic enzyme is bound to the mitochondrial inner membrane and harbors a unique lecithin-dependent activity. Here, we report an advanced purification method of the mammalian BDH applied to the liver enzyme from jerboa (Jaculus orientalis), a hibernating rodent adapted to extreme diet and environmental conditions. Results Purifying BDH from jerboa liver overcomes its low specific activity in mitochondria for further biochemical characterization of the e…