Search results for " RAF"
showing 10 items of 142 documents
Regulation of raft-derived endocytic pathways : studies on echovirus 1 and baculovirus
2009
Elina Kakkonen tutki väitöskirjassaan kahden erilaisen viruksen, echovirus 1:n ja bakuloviruksen soluun sisäänmenon reittejä ja säätelijöitä. Echovirus 1 (EV1) on ihmisiä infektoiva pikornavirus, joka voi aiheuttaa esimerkiksi aivokalvontulehdusta, sydänlihastulehdusta sekä lievempiä hengitystieinfektioita. Bakulovirus puolestaan on ihmisille vaaraton, hyönteisiä infektoiva virus, joka on osoittautunut lupaavaksi geeninsiirtokuljettajaksi geeniterapiassa.Virukset voivat käyttää soluun tunkeutumisessaan hyväkseen soluissa valmiina olevia erilaisia reittejä, joita solut itse tarvitsevat esimerkiksi ravintoaineiden sisäänottoon. Näin virukset pääsevät solun sisälle solun oman koneiston kuljett…
Blue-copper binding proteins of Medicago truncatula: Characterization of the expression of two genes related to the arbuscular mycorrhizal symbiosis
2009
International audience; Expression profiling of two paralogous arbuscular mycorrhizal (AM)-specific blue copper-binding gene (MtBcpla and MtBcp1b) isoforms was performed by real-time quantitative polymerase chain reaction in wild-type Medicago truncatula Jemalong 5 (J5) during the mycorrhizal development with Glomus intraradices for up to 7 weeks. Timecourse analysis in J5 showed that expression of both MtBcp1 genes increased continuously and correlated strongly with the colonization intensity and arbuscule content. MtPT4, selected as a reference gene of the functional plant-fungus association, showed a weaker correlation to mycorrhizal development. In a second experiment, a range of mycorr…
Role of RNA Motifs in RNA Interaction with Membrane Lipid Rafts: Implications for Therapeutic Applications of Exosomal RNAs
2021
RNA motifs may promote interactions with exosomes (EXO-motifs) and lipid rafts (RAFT-motifs) that are enriched in exosomal membranes. These interactions can promote selective RNA loading into exosomes. We quantified the affinity between RNA aptamers containing various EXO- and RAFT-motifs and membrane lipid rafts in a liposome model of exosomes by determining the dissociation constants. Analysis of the secondary structure of RNA molecules provided data about the possible location of EXO- and RAFT-motifs within the RNA structure. The affinity of RNAs containing RAFT-motifs (UUGU, UCCC, CUCC, CCCU) and some EXO-motifs (CCCU, UCCU) to rafted liposomes is higher in comparison to aptamers withou…
Binding of RNA Aptamers to Membrane Lipid Rafts: Implications for Exosomal miRNAs Transfer from Cancer to Immune Cells
2020
Intraluminal vesicles (ILVs) are released into the extracellular space as exosomes after the fusion of multivesicular bodies (MVBs) with the plasma membrane. miRNAs are delivered to the raft-like region of MVB by RNA-binding proteins (RBPs). RNA loading into exosomes can be either through direct interaction between RNA and the raft-like region of the MVB membrane, or through interaction between an RBP&ndash
Orthostatic hypotension and health outcomes: an umbrella review of observational studies
2019
To investigate potential relationships between orthostatic hypotension (OH) and negative health outcomes and mortality, through an umbrella review with integrated meta-analyses. Orthostatic hypotension is significantly associated with several negative outcomes in older people, but a suggestive evidence is available only for higher risk of coronary heart disease congestive heart failure, stroke, falls dementia, and all-cause mortality. Orthostatic hypotension seems to be significantly associated with several negative health outcomes in older people, even if only associations with coronary heart disease, congestive heart failure, stroke, falls, dementia, and all-cause mortality are supported …
Biomonitoring aktywny z zastosowaniem mchów w pobliżu Miasteczka Śląskiego
2017
Celem przeprowadzonych badań była ocena przyrostu stężeń wybranych analitów w próbkach mchów. Badania biomonitoringowe zanieczyszczenia aerozolu atmosferycznego prowadzono na terenie miejscowości Miasteczko Śląskie (województwo śląskie). Potencjalnym emitorem zanieczyszczeń na obszarze badań jest Huta Cynku „Miasteczko Śląskie”. Zastosowano metodę biomontoringu aktywnego (metoda woreczkowa), wykorzystując mchy z gatunku Pleurozium schreberi (rokietnik pospolity). Metale ciężkie w próbkach mchów oznaczono za pomocą absorpcyjnej spektrometrii atomowej (AAS). Wyniki badań zinterpretowano, wyznaczając współczynnik akumulacji względnej (RAF). Podczas prowadzenia eksperymentu uwzględniono odległo…
Wpływ warunków prowadzenia eksperymentu na wyniki badań biomonitoringowych z zastosowaniem mchów
2017
Ripostigli “misti” dalla Sicilia centro-meridionale
2011
It is remarkable the discovery in the hinterland of Sicily of three “mixed” hoards, including not only coins, but also of bronze scraps and small fragmentary objects. They demonstrate that in these centers, still in the 4th century BC, the coin was considered a worthless piece of metal and that other bronze objects were used as medium of exchange. The subject of further study is the so-called closet of Milocca-Milena (IGCH 2162), which is attributed to the site of Monte Raffe
Kolorismin voima : Rafael Wardin taiteen ja kuvallisen ajattelun taustatekijöitä
2010
Taidemaalari Rafael Wardi (s. 1928) tunnetaan parhaiten koloristisista maalauksistaan ja keltaisesta väristään. Hänet muistetaan myös sairauden ja vanhuuden esiin nostamisesta 1990-luvun lopulla sekä muotokuvaperinteen rikkomisesta presidentti Tarja Halosen muotokuvalla vuonna 2002. Selvitän pro gradussani Rafael Wardin taiteellisia lähtökohtia ja henkistä taustaa sekä tarkastelen hänen taiteensa kehitykseen vaikuttaneita asioita. Asetan Wardin hänen oman aikansa historialliseen ja kulttuuriseen kontekstiin. Tutkin hänen kotitaustaansa, opiskeluaikaansa matkoineen sekä 1940-50 -lukujen modernismin merkitystä Wardin taiteelle. Kartoitan myös juutalaisen kulttuurin perinnön vaikutusta hänen t…
El regeneracionismo educativo en Rafael Altamira : estudio aproximativo
1983
Acercamiento a la ideología y obra de Rafael Altamira, uno de los máximos representantes del Regeneracionismo Educativo hispano de finales del siglo XIX, principios del XX. Regeneracionismo Educativo hispano de Altamira. Aproximación histórica, social y cultural a la España de la época. Aproximación bibliográfica y análisis del tema educativo a través de su ideario pedagógico. Propuestas de Altamira para la regeneración de la educación tanto en la Enseñanza Primaria como en la Superior. Bibliografía. Documentos de archivo. Investigación histórica. Análisis descriptivo. La vida y obra de Altamira tienen su origen en el Krausismo positivo, que actúa desde unos principios y volación definidas …