Search results for " RR"

Evidence for a novel cytoplasmic processing event in ribosome maturation in the sea urchin Paracentrotus lividus

2010

In this work, we demonstrate the existence of a cytoplasmic processing step, never before described, involving both the pre-ribosomal subunits in the sea urchin Paracentrotus lividus. Northern-blot hybridization, primer extension, S1 mapping experiments and in situ hybridizations allowed us to demonstrate that cytoplasmic processed particles are successively re-imported into the nucleus, where maturation of their RNAs is completed prior to being exported to the cytoplasm. Our findings lead to the proposal of a new model of ribosome maturation and shuttling. Moreover, preliminary data from our laboratory suggest that the maturation pathway we propose in P. lividus may not be unique to the se…

GENOTYPIC AND PHENOTYPIC DIVERSITY OF LACTOBACILLUS ROSSIAE STRAINS ISOLATED FROM SOURDOUGH

2007

Cost effectiveness of boceprevir or telaprevir for previously treated patients with genotype 1 chronic hepatitis C.

2013

Background & Aims Randomised controlled trials (RCTs) show that triple therapy (TT) with peginterferon alfa, ribavirin, and boceprevir (BOC) or telaprevir (TVR) is more effective than peginterferon-ribavirin dual therapy (DT) in the treatment of genotype 1 (G1) chronic hepatitis C (CHC) patients with previous relapse (RR), partial response (PAR), and null-response (NR). We assess the cost-effectiveness of TT compared to no therapy in the treatment of patients previously treated with G1 CHC. Methods The available published literature provided the data source. The target population was made up of previously treated Caucasian patients with G1 CHC and these were evaluated over a lifetime horizo…

Fēču mikrobiotas īpatnības bērniem ar alerģiju

2021

Ievads.Zarnu mikrobiota ir daudzpusīgs un dinamisks process, kas sastāv no mikroorganismu sugu kopuma. Kuņģa-zarnu trakta mikrobiota ir viena no lielākajām un tā spēj darboties caur vairākām organismu asīm. Pašreizējie dati liecina, ka bērna agrīnai zarnu trakta mikrobiotai ir cieša saikne ar imūno sistēmu un alerģisko slimību attīstības risku. Noteicošais faktors bērna tālākai mikrobioma kolonizācijai ir pirmais dzīves gads, kad sākas intensīvākā mikroorganismu kolonizācija zarnās. Darba mērķis. Izpētīt fēču mikrobiotu bērniem ar un bez alerģijas, kā arī analizēt mikrobiotu ietekmējošus faktorus. Materiāli un metodes. Šķērsgriezuma pētījums tika veikts primārajos veselības aprūpes centros.…

Molecular phylogeny of Malagasy poison frogs, genus Mantella (Anura: Mantellidae): homoplastic evolution of colour pattern in aposematic amphibians

2002

Abstract We studied the evolution of colour pattern in Malagasy poison frogs, genus Mantella , a group of diurnal and toxic frogs endemic to Madagascar. Based on a phylogeny reconstructed using 1130 bp of the mitochondrial 16S rRNA gene, the genus can be divided into five species groups. Within some of these groups, interspecific genetic divergences were very low (1.2–2.8% sequence divergence) while colour patterns were markedly different. In contrast, Mantella madagascariensis and M . baroni , two species which show extremely similar dorsal coloration patterns, were not included in the same clade. This conclusion was supported by high bootstrap values and by significant rejection of altern…

New contribution to the systematic status of various Mediterranean scorpionfish, as inferred from a mitochondrial DNA sequence.

2014

This study investigated the molecular phylogeny of 6 Mediterranean species of scorpionfish, belonging to the Scorpaenidae and Sebastidae family. Neighbor-Joining and Maximum Parsimony phylogenetic analyse, based on 424 base pairs of partial mitochondrial DNA sequences of the 12S-rRNA gene, revealed 2 main clades. One clade is represented by the Scorpaena genera (with the species S. notata, S. porcus, and S. scrofa) and another clade consists of the genera Helicolenus, Pterois, and Scorpaenodes. The molecular phylogeny showed that the Scorpaenodes genus (sub-family Scorpaeninae) is found within the clade of the species belonging to the other two sub-families (Pteroninae and Sebastinae). This…

A round trip to the desert: In situ nanopore sequencing informs targeted bioprospecting

2021

Bioprospecting expeditions are often performed in remote locations, in order to access previously unexplored samples. Nevertheless, the actual potential of those samples is only assessed once scientists are back in the laboratory, where a time-consuming screening must take place. This work evaluates the suitability of using Nanopore sequencing during a journey to the Tabernas Desert (Spain) for forecasting the potential of specific samples in terms of bacterial diversity and prevalence of radiation- and desiccation-resistant taxa, which were the target of the bioprospecting activities. Samples collected during the first day were analyzed through 16S rRNA gene sequencing using a mobile labor…

Microbiota Variation Across Life Stages of European Field-Caught Anopheles atroparvus and During Laboratory Colonization: New Insights for Malaria Re…

2021

The potential use of bacteria for developing novel vector control approaches has awakened new interests in the study of the microbiota associated with vector species. To set a baseline for future malaria research, a high-throughput sequencing of the bacterial 16S ribosomal gene V3-V4 region was used to profile the microbiota associated with late-instar larvae, newly emerged females, and wild-caught females of a sylvan Anopheles atroparvus population from a former malaria transmission area of Spain. Field-acquired microbiota was then assessed in non-blood-fed laboratory-reared females from the second, sixth, and 10th generations. Diversity analyses revealed that bacterial communities varied …

Beyond archaea: The table salt bacteriome

2021

Commercial table salt is a condiment with food preservative properties by decreasing water activity and increasing osmotic pressure. Salt is also a source of halophilic bacteria and archaea. In the present research, the diversity of halotolerant and halophilic microorganisms was studied in six commercial table salts by culture-dependent and culture-independent techniques. Three table salts were obtained from marine origins: Atlantic Ocean, Mediterranean (Ibiza Island), and Odiel marshes (supermarket marine salt). Other salts supplemented with mineral and nutritional ingredients were also used: Himalayan pink, Hawaiian black, and one with dried vegetables known as Viking salt. The results of…

Impervious Surfaces Alter Soil Bacterial Communities in Urban Areas: A Case Study in Beijing, China

2018

The rapid expansion of urbanization has caused land cover change, especially the increasing area of impervious surfaces. Such alterations have significant effects on the soil ecosystem by impeding the exchange of gasses, water, and materials between soil and the atmosphere. It is unclear whether impervious surfaces have any effects on soil bacterial diversity and community composition. In the present study, we conducted an investigation of bacterial communities across five typical land cover types, including impervious surfaces (concrete), permeable pavement (bricks with round holes), shrub coverage (Buxus megistophylla Levl.), lawns (Festuca elata Keng ex E. Alexeev), and roadside trees (S…