Search results for " Recovery"

2020

Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a s…

People with Disabilities and Disasters

2017

Investigations have collectively established that disasters affect people with disabilities both disproportionately and negatively. In addition, perceived disability status is often associated with stigma, creating a separate and unique barrier across societies and cultures, which can augment vulnerability to disasters. Relevant research is reviewed by academic genesis areas in this chapter, namely; 1) disaster-focused research, 2) mental health, 3) epidemiology and public health, and 4) disability studies. Research emanating from these disciplines reflects different epistemological assumptions about disability as well as varying knowledge about disability as a social and cultural const…

Corticospinal Tract Integrity and Long-Term Hand Function Prognosis in Patients With Stroke

2019

Background: The restoration of hand function is an important goal for patients with stroke. This study investigated the relationship between corticospinal tract (CST) integrity and the functional status of the hand in patients with stroke 6 months after onset and evaluated which of the following values would be useful for predicting hand function: fiber number (FN), fractional anisotropy (FA) at the mid-pons, and FA at the pontomedullary junction. Methods: The present retrospective cross-sectional observational study assessed 44 patients with stroke who were able to walk without using a walking aid or orthosis. The final hand function results were classified into three groups: no recovery (…

The language of recovery

2020

Abstract The present study attempts to make a comparative analysis of two Spanish and American political speeches, which belong to two different debate traditions, in terms of the metaphors used. For that purpose, we analyze the Economy sections of the 2015 State of the Union Address in the US and in the 2015 State of the Nation Debate in Spain. The present study aims at answering the following research questions: What metaphors do President Obama and Prime Minister Rajoy use in the American and Spanish political speeches to convince their audiences of America’s and Spain’s economic victory? What are the similarities and differences between the representations depicted by metaphor use in th…

Thermal response test analysis for U-pipe vertical borehole heat exchangers under groundwater flow conditions

2021

[EN] Conventional models used in the analysis of thermal response test data only consider conduction as heat transfer mechanism. In cases where presence of groundwater is detected, convection heat transmission plays an important role, so its influence must be determined in the calculation of the effective thermal conductivity, usually overestimated in these situations, increasing its value the higher the power injected and the time elapsed. In this work, based on the data collected in a borehole located at UPV (Valencia) in which have been carried out three thermal response tests with different characteristics, has been implemented a variation of the finite line source model introducing an …

Plant-wide modelling in wastewater treatment: showcasing experiences using the Biological Nutrient Removal Model

2020

Abstract Plant-wide modelling can be considered an appropriate approach to represent the current complexity in water resource recovery facilities, reproducing all known phenomena in the different process units. Nonetheless, novel processes and new treatment schemes are still being developed and need to be fully incorporated in these models. This work presents a short chronological overview of some of the most relevant plant-wide models for wastewater treatment, as well as the authors' experience in plant-wide modelling using the general model BNRM (Biological Nutrient Removal Model), illustrating the key role of general models (also known as supermodels) in the field of wastewater treatment…

TECHNOLOGIES FOR THE BUILT HERITAGE ENHANCEMENT AND TOOLS FOR THE LOCAL ECONOMIC DEVELOPMENT

2012

Cultural and environmental goods are a “structured value capital” with its own distinctive elements of identity and authenticity. The need to give centrality to these territorial resources means to improve their keeping and protection processes and meantime to increase their usage and their sustainable development. So from the starting point of the sentence that the cultural heritage can determine positive falls on local development processes and that resources must be managed, organized and communicated following integrated logics, we need to focus those theoretical assumptions and those operative methodologies useful to start a clever circle between cultural heritage and economic developm…

Impact of t-PA administration on brain BDNF levels in physiological conditions and in circulating BDNF levels in ischemic conditions : Human and anim…

2014

Our objective was to test the hypothesis that the beneficial effect of the administration of the recombinant form of tissue plasminogen activator (rt-PA) in ischemic stroke patient not only results from its fibrinolytic activity but also from its ability to increase brain-derived neurotrophic factor (BDNF) in the brain. To this end, we conducted an initial study to evaluate the effect of rt-PA on brain BDNF levels in healthy animals. In a second study, we investigated the effect of rt-PA on serum BDNF levels in ischemic stroke patients and in animals subjected to permanent focal cerebral ischemia. Blood samples were obtained from patient on admission (D0), D1, D7 and D90 after stroke and in…

Renal Function Recovery Strategies Following Marathon in Amateur Runners

2022

Long distance races have a physiological impact on runners. Up to now, studies analyzing these physiological repercussions have been mainly focused on muscle and cardiac damage, as well as on its recovery. Therefore, a limited number of studies have been done to explore acute kidney failure and recovery after performing extreme exercises. Here, we monitored renal function in 76 marathon finishers (14 females) from the day before participating in a marathon until 192 h after crossing the finish line (FL). Renal function was evaluated by measuring serum creatinine (sCr) and the glomerular filtration rate (GFR). We randomly grouped our cohort into three intervention groups to compare three dif…

Active recovery shows favorable IGF-I and IGF binding protein responses following heavy resistance exercise compared to passive recovery

2019

IGF-I and IGFBPs have important physiological modulatory effects and this study sought to examine the influence of active vs. passive recovery following a heavy resistance exercise on IGF-I and IGF binding protein (IGFBP) recovery responses. It was hypothesized that increased IGF-I and decreased inhibitory IGFBPs during active recovery may be reflective of cascades promoting physiological recovery. 18 untrained men ((AR n = 7, PR n = 11), age: 26 ± 4 years, height: 174 ± 8 cm, body mass: 75 ± 13 kg) performed either a protocol-specific 10 × 10 × 30% 1RM active (AR) or passive recovery (PR) session following a heavy resistance exercise session performed on a leg press device (10 × 10 1RM). M…