Search results for " Regulation"
showing 10 items of 3187 documents
Intratumoral Heterogeneity for hsp90β mRNA Levels in a Breast Cancer Cell Line
1997
BC-3A and BC-61 are two breast cancer cell lines that have been cloned from parental 8701-BC cells and exhibit different biosynthetic, proliferative, and invasive properties in vitro. In the attempt to search whether alterations in the profiles of gene expression could be detected, we have submitted both cytotypes to identification of differentially expressed cDNAs. In addition, steroid hormone receptor mRNA arrays and in vivo tumorigenesis of the two lines have been checked. The technique used allowed identification of changes in the expression of the 90-kD heat shock protein-beta (hsp90beta) which is prominently down-regulated in BC-61 cells. Because we have also found that these cells, w…
Short-term response to waterlogging in Quercus petraea and Quercus robur: A study of the root hydraulic responses and the transcriptional pattern of …
2015
International audience; We characterized the short-term response to waterlogging in Quercus petraea (Matt.) Liebl. and Quercus robur L. as the initial response towards their known long-term differences in tolerance to waterlogging. One-month old seedlings were subjected to hypoxic stress and leaf gas exchange, shoot water potential (Psi(s)) and root hydraulic conductivity (Lp(r)) were measured. In parallel, the expression of nine aquaporins (AQPs) along the primary root was analysed by quantitative RT-PCR. Results showed a similar reduction in net assimilation (A) and stomatal conductance (g(s)) for the two species. Notably, the response of Lpr differed temporally between the two species. Q…
The Sensor Kinase DctS Forms a Tripartite Sensor Unit with DctB and DctA for Sensing C4-Dicarboxylates in Bacillus subtilis
2013
The DctSR two-component system of Bacillus subtilis controls the expression of the aerobic C4-dicarboxylate transporter DctA. Deletion of DctA leads to an increased dctA expression. The inactivation of DctB, an extracellular binding protein, is known to inhibit the expression of dctA. Here, interaction between the sensor kinase DctS and the transporter DctA as well as the binding protein DctB was demonstrated in vivo using streptavidin (Strep) or His protein interaction experiments (mSPINE or mHPINE), and the data suggest that DctA and DctB act as cosensors for DctS. The interaction between DctS and DctB was also confirmed by the bacterial two-hybrid system (BACTH). In contrast, no indicati…
Long Non Coding RNA H19: A New Player in Hypoxia-Induced Multiple Myeloma Cell Dissemination
2019
The long non-coding RNA H19 (lncH19) is broadly transcribed in the first stage of development and silenced in most cells of an adult organism
REGULATORY ELEMENTS OF THE LEUKAEMIA INHIBITORY FACTOR (LIF) PROMOTER IN MURINE BONE MARROW STROMAL CELLS
1999
Leukaemia inhibitory factor (LIF) plays an important role as a haematopoietically active cytokine. As described earlier in a murine model, interleukin 1 (IL-1) induced LIF mRNA and protein expression. We utilized the murine cell line +/+-1.LDA11 to further define regulatory mechanisms of LIF expression in bone marrow stromal cells. The production of LIF mRNA is stimulated by IL-1beta, TNF-alpha, and the cAMP analogue 8-bromoadenosine 3':5'-monophosphate (8BrcAMP). LIF mRNA expression is controlled at the transcriptional level. Different fragments from -542 to -45 bp 5' upstream of the transcriptional start site of the murine LIF gene were fused to the luciferase gene. All LIF-promoter lucif…
Some reflections on non-contractual obligations in cyberspace considering the Rome II regulation
2014
Internet penetration has created a revolutionary change in people's communication nowadays. By creating immaterial and elusive cyberspace, without borders and limits of communication a three-dimensional territorial concept has been transformed by globalizing social relations and reducing importance of regional or national dimensions. All situations taking place on the Internet also occur somewhere in the reality. Sometimes it is difficult to measure whether the events are random or unrelated to particular events. Technological progress offers new possibilities to change a user's real location either consciously or subconsciously. However, the law is highly territorial in nature. This articl…
Superparamagnetic γ-Fe2O3 nanoparticles with tailored functionality for protein separation
2007
Polymer coated superparamagnetic gamma-Fe(2)O(3) nanoparticles were derivatized with a synthetic double-stranded RNA [poly(IC)], a known allosteric activator of the latent (2-5)A synthetase, to separate a single 35 kDa protein from a crude extract which cross reacted with antibodies raised against the sponge enzyme.
Definitions of «Sustainability» and «Sustainable Technology». A Seemiotic and Narrative Approach on Agricultural Regulations
2018
In this paper, we focus on food production guidelines provided by two European sustainable regulations : the European Union (EU) Organic and the Biodiversity Friend certification. Drawing upon recent developments in the study of "technologies of law", this chapter seeks to examine the role and the meaning of technology in the expanding sector of sustainable standards in food production. Our claim is that agricultural regulations and certifications are semiotic devices which performatively define different technological systems which channel “human” and “non-human” forces in order to smooth out tensions between environmental and economic constraints. In the first paragraph, we show how cruci…
Regulating and Deregulating Sharing Mobility in Europe
2020
This chapter gives an account of the many legal issues related to the rise of sharing mobility in Europe, as part of the activities carried out within the 2018–2020 Jean Monnet Project “Regulating and Deregulating Sharing Mobility in Europe” (RIDER). In the first part, it outlines the most fundamental economic transformations that make the case for regulating sharing mobility services quite different from conventional transport. By also referring to the activities carried out during the project, it identifies those emerging legal issues stemming from the advent of sharing mobility, in order to categorize the competing claims that regulators should be considering when facing these innovative…
Municipal Building Regulations for Energy Efficiency in Southern Italy
2014
The building sector is still one of the most energy consuming sectors in Italy, like developed countries in Europe. At European level, the main policy driver related to the energy use in buildings is the Energy Performance of Buildings Directive (EPBD, 2002/91/EC) and its recast. Through the EPBD in- troduction, requirements for certification, inspections, training or renovation are now imposed in Member States. In order to fulfill the expected changes, local regulations are a key factor aiming at sustainable territorial planning. It is thus required support the issue of local rules at municipal level in order to guide local administrators and technicians and to limit discretional power of …