Search results for " Regulation"
showing 10 items of 3187 documents
Derepressing muscleblind expression by miRNA sponges ameliorates myotonic dystrophy-like phenotypes in Drosophila
2016
AbstractMyotonic Dystrophy type 1 (DM1) originates from alleles of the DMPK gene with hundreds of extra CTG repeats in the 3′ untranslated region (3′ UTR). CUG repeat RNAs accumulate in foci that sequester Muscleblind-like (MBNL) proteins away from their functional target transcripts. Endogenous upregulation of MBNL proteins is, thus, a potential therapeutic approach to DM1. Here we identify two miRNAs, dme-miR-277 and dme-miR-304, that differentially regulate muscleblind RNA isoforms in miRNA sensor constructs. We also show that their sequestration by sponge constructs derepresses endogenous muscleblind not only in a wild type background but also in a DM1 Drosophila model expressing non-co…
Sense and Antisense DMPK RNA Foci Accumulate in DM1 Tissues during Development.
2015
International audience; Myotonic dystrophy type 1 (DM1) is caused by an unstable expanded CTG repeat located within the DMPK gene 3'UTR. The nature, severity and age at onset of DM1 symptoms are very variable in patients. Different forms of the disease are described, among which the congenital form (CDM) is the most severe. Molecular mechanisms of DM1 are well characterized for the adult form and involve accumulation of mutant DMPK RNA forming foci in the nucleus. These RNA foci sequester proteins from the MBNL family and deregulate CELF proteins. These proteins are involved in many cellular mechanisms such as alternative splicing, transcriptional, translational and post-translational regul…
Deficiency of Nrf2 accelerates the effector phase of arthritis and aggravates joint disease
2011
14 páginas, 8 figuras, 1 tabla.-- et al.
Sequence analysis of the DRB1 promoter reveals limited polymorphism with no influence on gene expression.
2001
HLA-class II promoters contain a set of conserved regulatory regions necessary for constitutive and induced gene expression. For the HLA-DQB as well as for the DRB1 promoter sequence, polymorphisms with influence on gene expression have been reported. In contrast to these data we could show that there is very limited allele-specific polymorphism among the HLA-DRB1 promoter alleles. In a long range PCR we amplified a DNA sequence containing the promoter and the second exon of the DRB1 gene in one fragment. Nested PCR products of this PCR fragment for the promoter and for the second exon were analysed by DNA sequencing to allow the linkage of a promoter to its DR allele. Most investigated DRB…
Development of the first marmoset-specific DNA microarray (EUMAMA): a new genetic tool for large-scale expression profiling in a non-human primate
2007
Abstract Background The common marmoset monkey (Callithrix jacchus), a small non-endangered New World primate native to eastern Brazil, is becoming increasingly used as a non-human primate model in biomedical research, drug development and safety assessment. In contrast to the growing interest for the marmoset as an animal model, the molecular tools for genetic analysis are extremely limited. Results Here we report the development of the first marmoset-specific oligonucleotide microarray (EUMAMA) containing probe sets targeting 1541 different marmoset transcripts expressed in hippocampus. These 1541 transcripts represent a wide variety of different functional gene classes. Hybridisation of …
Extracellular matrix regulation of PTHrP and PTH/PTHrP receptor in a human breast cancer cell line
1999
AbstractIt was previously reported that 8701-BC breast cancer cells express the gene for parathyroid hormone-related peptide (PTHrP) and its cognate receptor (PTHrP-R), and release immunoreactive PTHrP in the extracellular medium; it was also found that PTHrP, in turn, exerts a role on the proliferative and invasive behavior in vitro of the same cell line. On the other hand, evidence has been produced that adhesion of 8701-BC cells onto different collagen substrates influences in various ways a number of phenotypic expressions, such as cell growth, motility, invasion of reconstituted basement membrane and production of lytic enzymes of the extracellular matrix (ECM). In light of these previ…
Music-Related Nostalgic Experiences of Young Migrants
2013
Music is a powerful means for constructing identity – both formulating and remembering who one is – and migration can be seen as a particular situation creating challenges for this sense of identity. This qualitative study focused on investigating music-related nostalgic experiences of young migrants. The aim of the study was to identify features characteristic to music-related nostalgia particularly in relation to the experience of migration. Ten university students (Iranians currently living in migration) participated in the study. Data was collected through spoken interviews with an additional short questionnaire and a task to listen to self-selected nostalgic music. The interviews focus…
Internet two sided market regulation: A zero-ratong analysis from a theoretical and experimental approach
2022
El objetivo de esta tesis es estudiar las prácticas discriminatorias empleadas en el Mercado de Internet. Dichas prácticas han sido reguladas por la neutralidad de red en medio de un amplio y polémico debate político, económico y técnico. Con esta investigación, hemos querido analizar los efectos de los servicios de zero-rating desde una perspectiva teórica y experimental. Así mismo, proponemos un replanteamiento de la neutralidad de red a través de un estudio experimental que replica las interacciones entre agentes en el actual ecosistema digital. Capítulo 2: El objetivo de este capítulo es estudiar el efecto de los contratos de zero-rating en un mercado bilateral de duopolio de Internet. …
The corticotrophin-releasing factor/urocortin system regulates white fat browning in mice through paracrine mechanisms.
2015
Objectives:\ud The corticotrophin-releasing factor (CRF)/urocortin system is expressed in the adipose tissue of mammals, but its functional role in this tissue remains unknown.\ud \ud Methods:\ud Pharmacological manipulation of the activity of CRF receptors, CRF1 and CRF2, was performed in 3T3L1 white pre-adipocytes and T37i brown pre-adipocytes during in vitro differentiation. The expression of genes of the CRF/urocortin system and of markers of white and brown adipocytes was evaluated along with mitochondrial biogenesis and cellular oxygen consumption. Metabolic evaluation of corticosterone-deficient or supplemented Crhr1-null (Crhr1−/−) mice and their wild-type controls was performed alo…
Vecākā pirmsskolas vecuma bērnu satiksmes noteikumu apguves sekmēšana pedagoģiskajā procesā
2018
Darba autors: Alvīne Mārtinsone Darba temats: Vecākā pirmsskolas vecuma bērnu satiksmes noteikumu apguves sekmēšana pedagoģiskajā procesā. Darba mērķis: analizēt un pētīt vecākā pirmsskolas vecuma bērna satiksmes noteikumu apguves sekmēšanas iespējas rotaļās (rotaļnodarbībā). Lai atklātu izvirzīto mērķi, pētījumā tika analizētas dažādas pedagoģiskās, psiholoģiskās literatūras analīzes. Pirmajā daļā tiek apskatīti dažādu psihologu, pedagogu konkrētā vecuma posma bērnu raksturojums. Apskatītas vadlīnijas un Ministru kabineta noteikumi, kā arī dažādu pedagogu, psihologu nosacījumi, kuri satiksmes noteikumi jāmāca pirmsskolas vecuma bērniem. Lai noskaidrotu, kādi pedagoģiskie paņēmieni sekmēs b…