Search results for " SIT"

showing 10 items of 2985 documents

Cellular distribution of nicotinic acetylcholine receptor subunit mRNAs in the human cerebral cortex as revealed by non-isotopic in situ hybridizatio…

1994

The pharmacology of telencephalic nicotinic acetylcholine receptors (nAChRs) has become an important issue in recent years. While in the human brain a direct pharmacological assessment is difficult to achieve the visualization of nAChRs has been enabled by histochemical techniques providing an ever increasing and improving resolution. Receptor autoradiography was used to visualize binding sites on the level of cortical layers whereas immunohistochemistry has allowed for the cell type-specific and ultrastructural localization of receptor protein. Further investigations have to elucidate the cellular sites of NAChR biosynthesis by visualizing subunit-specific transcripts. Using autopsy sample…

AdultMaleIn situ hybridizationBiologyReceptors NicotinicCellular and Molecular NeuroscienceCortex (anatomy)medicineHumansRNA MessengerReceptorMolecular BiologyIn Situ HybridizationAcetylcholine receptorAgedCerebral CortexNeuronsSequence Homology Amino AcidHuman brainRNA ProbesMiddle AgedAlkaline PhosphataseNicotinic acetylcholine receptormedicine.anatomical_structureNicotinic agonistCerebral cortexFemaleNeuroscienceBrain research. Molecular brain research
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Cancer incidence in Italian contaminated sites

2014

INTRODUCTION: The incidence of cancer among residents in sites contaminated by pollutants with a possible health impact is not adequately studied. In Italy, SENTIERI Project (Epidemiological study of residents in National Priority Contaminated Sites, NPCSs) was implemented to study major health outcomes for residents in 44 NPCSs. METHODS: The Italian Association of Cancer Registries (AIRTUM) records cancer incidence in 23 NPCSs. For each NPCSs, the incidence of all malignant cancers combined and 35 cancer sites (coded according to ICD-10), was analysed (1996-2005). The observed cases were compared to the expected based on age (5-year period,18 classes), gender, calendar period (1996-2000; 2…

AdultMaleIncidencecontaminated sites; Cancer incidenceAdult; Aged; Environmental Exposure; Environmental Pollution; Female; Humans; Incidence; Italy; Male; Middle Aged; Neoplasmscancerc incidencecancerc incidence; contaminated sites; Cancer in ItalyEnvironmental ExposureMiddle AgedSettore MED/42 - Igiene Generale E ApplicataContaminated sitesCancer incidence; Contaminated sites; Environmental pollution;ItalyNeoplasmsHumansFemaleEnvironmental Pollutioncontaminated siteCancer in ItalyCancer incidenceAged
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FISH screening of aneuploidies in preimplantation embryos to improve IVF outcome

2005

Preimplantation genetic diagnosis (PGD) has transformed the approach to the infertility patient in the IVF setting. Although the principal applications of PGD have been to prevent the transmission of sex-linked diseases, in time and with growing knowledge of the chromosomal abnormalities observed in preimplantation embryos, its applications have widened. Nowadays, apart from its implications in the prevention of transmission of chromosomal and genetic abnormalities, PGD is being used with increased frequency to improve the IVF outcome in patients with advanced maternal age (or =38 years of age), recurrent miscarriage (or =2 miscarriages), recurrent IVF failure (or =3 failed IVF attempts) an…

AdultMaleInfertilityAbortion Habitualmedicine.medical_specialtyTime FactorsBiopsyAneuploidyFertilization in VitroBiologyAbortionPreimplantation genetic diagnosisChromosomesMale infertilityPregnancyRecurrent miscarriagemedicineHumansAdvanced maternal ageIn Situ Hybridization FluorescenceInfertility MaleChromosome AberrationsGynecologyPregnancyObstetricsPregnancy OutcomeObstetrics and GynecologyMiddle AgedAneuploidyEmbryo Mammalianmedicine.diseaseBlastocystTreatment OutcomeReproductive MedicineInfertilityFemaleInfertility FemaleMaternal AgeDevelopmental BiologyReproductive BioMedicine Online
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Intraocular pressure after excimer laser myopic refractive surgery

2001

The aim of this study was to determine whether intraocular pressure (IOP), as measured by Goldmann applanation or non-contact tonometry, shows systematic changes in patients who have undergone photorefractive keratectomy (PRK) or laser in situ keratomileusis (LASIK). IOP was measured by central Goldmann and non-contact tonometry in 54 patients pre and post-PRK, and in 43 patients pre- and post-LASIK. An interval of 12 months was allowed after surgery. Patients were selected to have one of four specific initial values of refractive error (-2.5, -5.0, -7.5 and -10.0 D). Fellow unoperated eyes were used as controls. A paired Student's t-test and a one-way ANOVA test were used for statistical a…

AdultMaleIntraocular pressuremedicine.medical_specialtyRefractive errorgenetic structuresmedicine.medical_treatmentEye diseaseKeratomileusis Laser In SituContext (language use)KeratomileusisPhotorefractive KeratectomySensitivity and SpecificityTonometry OcularRefractive surgeryOphthalmologyMyopiamedicineHumansIntraocular PressureAnalysis of Variancebusiness.industryLASIKmedicine.diseaseeye diseasesSensory SystemsPhotorefractive keratectomySurgeryOphthalmologyFemaleLasers Excimersense organsbusinessOptometryOphthalmic and Physiological Optics
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Event-related potentials reflecting the processing of phonological constraint violations

2009

How are violations of phonological constraints processed in word comprehension? The present article reports the results of an event-related potentials (ERP) study on a phonological constraint of German that disallows identical segments within a syllable or word (CC(i)VC(i)). We examined three types of monosyllabic late positive CCVC words: (a) existing words [see text], (b) wellformed novel words [see text] and component (c) illformed novel words [see text] as instances of Obligatory Contour Principle non-word (OCP) violations. Wellformed and illformed novel words evoked an N400 effect processing in comparison to existing words. In addition, illformed words produced an enhanced late posteri…

AdultMaleLinguistics and LanguageSociology and Political ScienceSpeech recognitionWord processingPhonological wordVocabularyLanguage and LinguisticsYoung AdultSpeech and HearingCognitionPhoneticsHumansSpeechDeoxyribonucleases Type II Site-SpecificEvoked PotentialsLate positive componentLanguageMathematicsPhonotacticsBrainElectroencephalographyGeneral MedicineN400LinguisticsAcoustic StimulationWord recognitionSpeech PerceptionFemaleSyllableObligatory Contour PrincipleLanguage and Speech
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Translocation (X;18) in a Biphasic Synovial Sarcoma with Morphologic Features of Neural Differentiation

1998

The authors report a recurred neoplasm showing distinctive histologic, immunophenotypic, and ultrastructural features characteristic of biphasic synovial sarcoma with neural differentiation. The features include areas with a growth pattern of densely packed spindle cells in irregularly intersecting, broad fascicles, diffuse vimentin and HBA 71 immunoreactivity, expression of S-100 protein, and other neural markers. Moreover, areas with glandular structures and cellular expression of cytokeratin and epithelial membrane antigen were noted. Additionally, areas of neural-like growth pattern were positive for neuron-specific enolase, HNK-1, and protein gene product 9.5. Furthermore, cytogenetic …

AdultMaleLung NeoplasmsX ChromosomeBiphasic Synovial SarcomaEnolaseSoft Tissue NeoplasmsChromosomal translocationVimentinPolymerase Chain ReactionTranslocation GeneticImmunophenotypingPathology and Forensic MedicineGene productSarcoma SynovialCytokeratinTumor Cells CulturedmedicineHumansMolecular BiologyIn Situ Hybridization FluorescenceNeuronsmedicine.diagnostic_testbiologyChemistryCell DifferentiationPatellaCell BiologyMolecular biologyReverse transcription polymerase chain reactionKaryotypingbiology.proteinChromosomes Human Pair 18Fluorescence in situ hybridizationDiagnostic Molecular Pathology
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Long-time expression of DNA repair enzymes MGMT and APE in human peripheral blood mononuclear cells.

2001

The DNA repair enzymes O6-methylguanine-DNA methyltransferase (MGMT) and apurinic/apyrimidinic endonuclease (APE, also known as Ref-1) play an important role in cellular defense against the mutagenic and carcinogenic effects of DNA-damaging agents. Cells with low enzyme activity are more sensitive to induced DNA damage and may confer a higher carcinogenic risk to the individuals in question. To study the level of variability of MGMT and APE expression in human, we analyzed in a long-time study MGMT and APE expression in peripheral blood mononuclear cells (PBMC) from healthy individuals. The data revealed high inter- and intraindividual variability of MGMT but not of APE. For MGMT, the inter…

AdultMaleMethyltransferaseTime FactorsDNA LigasesDNA repairDNA damageHealth Toxicology and MutagenesisBlotting WesternCarbon-Oxygen LyasesBiologyToxicologyPeripheral blood mononuclear cellMonocytesEndonucleaseO(6)-Methylguanine-DNA MethyltransferaseGene expressionDNA-(Apurinic or Apyrimidinic Site) LyaseHumansneoplasmsCarcinogenSmokingGeneral MedicineDNA-(apurinic or apyrimidinic site) lyaseMolecular biologydigestive system diseasesDeoxyribonuclease IV (Phage T4-Induced)biology.proteinFemaleArchives of toxicology
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Prevalence of surgical site infections before and after the implementation of a multimodal infection control programme

2012

Objectives: In order to assess the prevalence of surgical site infections (SSIs) before and after the implementation of a multimodal infection control programme including the realization of a campaign to increase compliance with guidelines for antimicrobial prophylaxis, we designed and conducted the present study involving all 20 of the surgical departments of a large teaching hospital in Catania, Italy. Patients and methods: SSI definitions of the Hospital in Europe Link for Infection Control through Surveillance (HELICS) protocol were used in four 1 day point-prevalence surveys. After the first survey, an infection control programme was implemented involving the active commitment of surge…

AdultMaleMicrobiology (medical)medicine.medical_specialtyEvidence-based practiceSettore MED/42 - Igiene Generale E ApplicataSurgical sitePrevalencemedicineHumansSurgical Wound InfectionInfection controlPharmacology (medical)Antibiotic prophylaxisIntensive care medicineAgedPharmacologyInfection Controlbusiness.industryAntibiotic ProphylaxisMiddle AgedHospitalsantimicrobial prophylaxis guidelines compliance SSIsAnti-Bacterial AgentsInfectious disease prevention / controlInfectious DiseasesItalyFemaleGuideline AdherenceHealth Services ResearchbusinessJournal of Antimicrobial Chemotherapy
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HIV-related non-Hodgkin lymphomas affecting the oral cavity: a clinicopathologic study of 11 cases

2021

HIV-related non-Hodgkin lymphomas of the oral cavity are rare lesions with aggressive clinical behaviour. The aim of this study is to describe the clinicopathological features of a series of HIV-related oral non-Hodgkin lymphomas. Eleven cases of oral lymphomas affecting HIV-positive patients were retrieved from 2012 to 2019. Clinicopathological features regarding age, sex, tumour location, clinical presentation, laboratory findings, disease stage and follow-up were obtained. Histologic, immunohistochemical and in situ hybridization for EBV detection were done for diagnosis confirmation. Overall survival was estimated by Kaplan?Meier curve. Males predominated, with a mean age of 40.3 years-…

AdultMaleMouthimpacted toothLymphoma Non-HodgkinHIV InfectionsOtorhinolaryngologypanoramic radiographydental cariesHumansSurgerythird molarLymphoma Large B-Cell Diffusedigital radiographyGeneral DentistryUNESCO:CIENCIAS MÉDICASIn Situ HybridizationMedicina Oral Patología Oral y Cirugia Bucal
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Optical and Visual Simulation of Standard and Modified Spherical Aberration Implantable Collamer Lens Post Myopic LASIK Surgery

2013

To evaluate the visual quality achieved in patients undergoing standard or modified implantable Collamer lens (ICL) to correct residual myopic error after laser-assisted in situ keratomileusis (LASIK) surgery.The adaptive optics visual simulator was used to simulate LASIK surgery of moderate and high myopia with a myopic regression corrected by a standard and modified ICL. Visual acuity (VA) and contrast sensitivity (CS) were measured in 14 subjects at 3- and 4.5-mm pupil. Point spread function and simulated retinal images were calculated.Comparing LASIK plus standard ICL and LASIK plus modified ICL simulations, for moderate myopia, VA improvement was less than 1 line in all VA contrasts an…

AdultMaleOptics and PhotonicsPhakic Intraocular Lensesmedicine.medical_specialtyCorneal Wavefront AberrationAdolescentgenetic structuresmedicine.medical_treatmentKeratomileusis Laser In SituVisual AcuityKeratomileusisPhakic intraocular lensContrast SensitivityYoung AdultLens Implantation IntraocularAberrometryMyopiamedicineHumansIn patientPostoperative PeriodImplantable collamer lensbusiness.industryAberrometryLASIKGeneral Medicineeye diseasesSurgeryOphthalmologySpherical aberrationOptometryFemaleLasers ExcimerbusinessEuropean Journal of Ophthalmology
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