Search results for " STRESS"
showing 10 items of 3936 documents
Glicerīna cikla ietekme uz maizes rauga šūnu fenotipu
2017
Šī darba mērķis bija novērtēt redoks kofaktora NADPH inženierijas mēģinājuma ietekmi uz rekombinantu Saccharomyces cerevisiae celmu fenotipu eksponenciālās augšanas laikā. Šajos CEN.PK2-1C rekombinantajos celmos tika ekspresēta NADP+ atkarīga glicerīna dehidrogenāze (Gld2p) ar mērķi atgriezt daļu glicerīna glikolīzē, kombinācijā ar glutationa antioksidatīvās sistēmas un NADPH kofaktora reģenerāciju. Rekombinantajiem celmiem tika noteikta subletālo stresu noturība, NADPH/NADP+ kofaktora un GSH/GSSG savstarpējās relatīvās attiecības, izmantojot spektrofotometriju un fluorometriju, kā arī rekombinantu augšanas dinamika ar ksilozi kā vienīgo oglekļa avotu. Darbā novērots, ka izveidotais „glicer…
Characterization of Sicilian Olive Genotypes by Multivariate Analysis of Leaf and Fruit Chemical and Morphological Properties
2013
Leaf and fruit size and shape were measured and mannitol, glucose, sucrose and malic acid were quantified in leaf, bark and fruit of 25 Sicilian olive genotypes. Multivariate analysis was used to individuate groups with similar chemical composition and morphological traits suggesting potential for stress tolerance and/or oil yield and quality. Mannitol content varied greatly among genotypes and was the most abundant carbohydrate in leaf and bark, whereas glucose was the most abundant in fruit. Sucrose and malic acid were generally low indicating a marginal role in olive tissues. Mannitol and glucose were directly related in both leaf and fruit tissues. Genotypes also differed for carbohydra…
Using exomarkers to assess mitochondrial reactive species in vivo
2014
Background:\ud The ability to measure the concentrations of small damaging and signalling molecules such as reactive oxygen species (ROS) in vivo is essential to understanding their biological roles. While a range of methods can be applied to in vitro systems, measuring the levels and relative changes in reactive species in vivo is challenging.\ud \ud Scope of review:\ud One approach towards achieving this goal is the use of exomarkers. In this, exogenous probe compounds are administered to the intact organism and are then transformed by the reactive molecules in vivo to produce a diagnostic exomarker. The exomarker and the precursor probe can be analysed ex vivo to infer the identity and a…
Endocrine effects of sauna bath
2020
Sauna bath brings about numerous acute changes in hormone levels, partly akin to other stressful situations, partly specific for sauna. Norepinephrine increases in those accustomed to sauna bath. Sweating increases the production of antidiuretic hormone, and the renin-angiotensin system becomes activated. Of the anterior pituitary hormones, growth hormone (GH) and prolactin (PRL) secretion is increased. Also β-endorphin has been frequently reported to increase, whereas the responses of ACTH and cortisol are variable, probably depending on the type of sauna exposure. Sperm production decreases in particular in sauna-naïve men, but reduced fertility has not been associated with regular sauna …
Exogenous Growth Regulators and Water Stress Enhance Long-Term Storage Quality Characteristics of Onion
2023
Exogenous growth regulators (GRs) play a crucial role in alleviating water stress and sustaining crop yields in water-stressed areas. However, their effects on onions post-harvest quality, particularly post-monsoon onion—often preferred for long-term storage—were never studied. Therefore, this led us to investigate the interaction between water stress and GRs on the physiochemical and functional quality attributes of onions during long-term storage (9 months, at 25 ± 1 °C and 65 ± 5% RH). Onion crop was raised under four water stress levels i.e., 1.00–0.85, 0.84–0.70, 0.69–0.40, and 0.39–0.10 IW: CPE, designated as no, low, medium, and s…
Prolonging in utero-like oxygenation after birth diminishes oxidative stress in the lung and brain of mice pups☆
2013
Background Fetal-to-neonatal transition is associated with oxidative stress. In preterm infants, immaturity of the antioxidant system favours supplemental oxygen-derived morbidity and mortality. Objectives To assess if prolonging in utero-like oxygenation during the fetal-to-neonatal transition limits oxidative stress in the lung and brain, improving postnatal adaptation of mice pups. Material and methods Inspiratory oxygen fraction (FiO2) in pregnant mice was reduced from 21% (room air) to 14% (hypoxia) 8–12 h prior to delivery and reset to 21% 6–8 h after birth. The control group was kept at 21% during the procedure. Reduced (GSH) and oxidized (GSSG) glutathione and its precursors [γ-glut…
Applying the Anna Karenina principle for wild animal gut microbiota : temporal stability of the bank vole gut microbiota in a disturbed environment
2020
Gut microbiota play an important role in host health. Yet, the drivers and patterns of microbiota imbalance (dysbiosis) in wild animals remain largely unexplored. One hypothesised outcome of stress on animal microbiomes is a destabilised microbial community that is characterised by an increase in inter-individual differences compared with microbiomes of healthy animals, which are expected to be (i) temporally stable and (ii) relatively similar among individuals. This set of predictions for response of microbiomes to stressors is known as the Anna Karenina principle (AKP) for animal microbiomes. We examine the AKP in a wild mammal inhabiting disturbed environments by conducting a capture-mar…
Protective Effects of a Discontinuous Treatment with Alpha-Lipoic Acid in Obesity-Related Heart Failure with Preserved Ejection Fraction, in Rats
2020
Obesity induces hemodynamic and humoral changes that are associated with functional and structural cardiac remodeling, which ultimately result in the development of heart failure (HF) with preserved ejection fraction (HFpEF). In recent years, pharmacological studies in patients with HFpEF were mostly unsatisfactory. In these conditions, alternative new therapeutic approaches are necessary. The aim of our study was (1) to assess the effects of obesity on heart function in an experimental model and (2) to evaluate the efficacy of an alpha-lipoic acid (ALA) antioxidant treatment. Sprague-Dawley rats (7 weeks old) were either included in the control group (n = 6) or subjected to abdominal aorti…
Ischemia Cardiaca e Psicosomatica: ruolo di eventi stressanti e stili di vita
2009
Objective: the aim of our study was to evaluate the role of stressful events, lifestyles and various socio-environmental factors in the beginning of ischemic cardiac diseases, together with cardiovascular factors. Materials and methods: 64 patients with recent cardiac ischemia and 64 controls matched 1:1, according to their sex and age, have been evaluated. The study required the filling in of clinico-anamnestic reports and the evaluation of stressful events, using the Holmes Rahe scale. Results: in the 44% of the patients who had a heart ischemia, an emotional striking event occurred few day before, with a 28% incidence of work and family problems. The mean score of the Holmes Social Readj…