Search results for " Seconda"

Decoding vibrational states of Concanavalin A amyloid fibrils.

2015

International audience; Amyloid and amyloid-like fibrils are a general class of protein aggregates and represent a central topic in life sciences for their involvement in several neurodegenerative disorders and their unique mechanical and supramolecular morphological properties. Both their biological role and their physical properties, including their high mechanical stability and thermodynamic inertia, are related to the structural arrangement of proteins in the aggregates at molecular level. Significant variations may exist in the supramolecular organization of the commonly termed cross-β structure that constitutes the amyloid core. In this context, a fine knowledge of the structural deta…

Cholesterol facilitates interactions between α‐synuclein oligomers and charge‐neutral membranes

2015

AbstractOligomeric species formed during α-synuclein fibrillation are suggested to be membrane-disrupting agents, and have been associated with cytotoxicity in Parkinson’s disease. The majority of studies, however, have revealed that the effect of α-synuclein oligomers is only noticeable on systems composed of anionic lipids, while the more physiologically relevant zwitterionic lipids remain intact. We present experimental evidence for significant morphological changes in zwitterionic membranes containing cholesterol, induced by α-synuclein oligomers. Depending on the lipid composition, model membranes are either unperturbed, disrupt, or undergo dramatic morphological changes and segregate …

Self-assembly of 3,5-bis(ethoxycarbonyl)pyrazolate anions and ammonium cations of β-phenylethylamine or homoveratrylamine into hetero-double-stranded…

2009

Hydrogen-bonded double-stranded hetero-helices are formed when reacting sodium 3,5-bis(ethoxycarbonyl)pyrazolate with beta-phenethylammonium or homoveratrylammonium chloride, in which one of the strands is defined by the ammonium cations and the other one by the pyrazolate anions.

Characterization of liver cytokeratin as a major target antigen of anti-SLA antibodies.

1990

Abstract Anti-SLA antibodies characterize a newly defined subgroup of patients with autoimmune chronic active hepatitis. The aim of the present study was the immunochemical characterization of the target antigen(s) of anti-SLA antibodies. Anti-SLA-positive sera were found to contain high titres of anti-cytokeratin antibodies. In immunoblotting analyses with 100 000 × g supernatants of human liver homogenates (S-100) these sera recognized various proteins with a molecular mass of 40–60 kDa. These proteins were also recognized by monoclonal anti-cytokeratin antibodies. Two-dimensional co-electrophoresis and immunoblotting analysis of S-100 and liver cytokeratins showed that anti-SLA antibodie…

I microrganismi di interesse biotecnologico

2008

Polyclonal antibodies to mannan from yeast also recognize the carbohydrate structure of gp120 of the AIDS virus: an approach to raise neutralizing an…

1990

This study initiates a new method of developing an antigen which might be useful in the prevention of HIV-1 infection. Using a mannan preparation from Saccharomyces cerevisiae neutralizing antiserum was raised in rabbits which prevents HIV-1 infection in vitro up to a titre of 1:128. The corresponding antibody preparation neutralized the in vitro infectivity down to a concentration of 5 micrograms/ml. Analytical studies suggest that the antibodies are directed against the mannose residues of the HIV-1 glycoprotein (gp) 120 and its precursor gp 160.

Interaction of theEscherichia colitransporter DctA with the sensor kinase DcuS: presence of functional DctA/DcuS sensor units

2012

The aerobic Escherichia coli C(4) -dicarboxylate transporter DctA and the anaerobic fumarate/succinate antiporter DcuB function as obligate co-sensors of the fumarate responsive sensor kinase DcuS under aerobic or anaerobic conditions respectively. Overproduction under anaerobic conditions allowed DctA to replace DcuB in co-sensing, indicating their functional equivalence in this capacity. In vivo interaction studies between DctA and DcuS using FRET or a bacterial two-hybrid system (BACTH) demonstrated their interaction. DctA-YFP bound to an affinity column and was able to retain DcuS. DctA shows substantial sequence and secondary structure conservation to Glt(Ph), the Na(+)/glutamate sympo…

Les procédures d'orientation dans le système scolaire et leurs implications dans la compréhension des relations entre la formation et l'emploi

1981

International audience; Le texte ci-après cherche à explorer en quoi les procédures d'orientation qui sont fondamentales dans la constitution de la segmentation de la population scolaire sont importantes pour la compréhension des phénomènes d'orientation. Il comprend trois parties. La première propose une réflexion d'ordre théorique sur l'orientation au niveau de la classe de 3ème et tente de poser des questions objectives, ayant un contenu empirique opératoire. La seconde partie donne des éléments empiriques sur l'orientation à la fin de La c lasse de 3ème. L'ensemble des questions posées dans la première partie aurait dû y trouver une réponse. Toutefois, des raisons techniques ont retardé…

Le procédures d'orientation dans le système scolaire et leurs implications dans la compréhension des relations entre la formation et l'emploi - 3ème …

1981

International audience

L'insertion professionnelle des jeunes apprentis en Champagne-Ardenne en 1979

1983

Référence interne : 83006; Dans une première partie, nous nous proposons d'examiner la place relative de l'apprentissage en Champagne-Ardenne et en France, d'une part à travers l'évolution des effectifs sur plusieurs années ; et d'autre part, à travers son importance au sein de la population scolaire et la population active. Une deuxième partie décrit les principales caractéristiques de la population interrogée : de façon à approcher au mieux cette population, nous nous sommes appuyés sur les effectifs en année terminale de formation, aussi souvent que possible. Une troisième partie tente de dégager quelques données relatives à l'insertion professionnelle en 1979.