Search results for " South"

Trends of mean temperatures and warm extremes in northern tropical Africa (1961-2014) from observed and PPCA-reconstructed time series

2016

Trends in daily maximum (TX) and minimum (TN) temperatures and indices of warm extremes are studied in tropical North Africa, west of the eastern African highlands, from 1961 to 2014. The analysis is based on the concatenation and cross-checking of two observed databases. Due to the large number of missing entries (~25%), a statistical infilling using probabilistic principal component analysis was applied. Averaged over 90 stations, the linear trends of annual mean TX and TN equal respectively +0.021 °C/yr and +0.028 °C/yr. The frequency of very hot days (TX > 35°C) and tropical nights (TN > 20°C), as well as the frequency of daily TX and TN above the 90th percentile (p90) (“warm days” and …

Women’s empowerment and child mortality: the case of Bangladesh

2018

Cloning and characterization of the phenylalanyl-tRNA synthetase β subunit gene fromCandida albicans

1998

A Candida albicans expression library was constructed from RNA isolated from regenerating protoplasts. A 1.4-kb cDNA clone was used to isolate a genomic fragment. Sequence analysis revealed an open reading frame of 593 amino acids with an overall identity of 63.6% with the phenylalanyl-tRNA synthetase beta subunit (FRS1) of Saccharomyces cerevisiae. We named it CaFRS1. It is located in a single copy in chromosome R, SfiI fragment M. Its expression showed a decrease during the cell wall regeneration process in protoplasts of both yeast and mycelial cells of C. albicans, suggesting its requirement thereof in initial steps of the cell wall synthesis.

Artificial chromosomes for antibiotic-producing actinomycetes.

2000

Bacteria belonging to the order Actinomycetales produce most microbial metabolites thus far described, several of which have found applications in medicine and agriculture. However, most strains were discovered by their ability to produce a given molecule and are, therefore, poorly characterized physiologically and genetically. Thus, methodologies for genetic manipulation of actinomycetes are not available and efficient tools have been developed for just a few strains. This constitutes a serious limitation to applying molecular genetics approaches to strain development and structural manipulation of microbial metabolites. To overcome this hurdle, we have developed bacterial artificial chrom…

DNA methylation and histone acetylation of rat methionine adenosyltransferase 1A and 2A genes is tissue-specific.

2000

Methionine adenosyltransferase (MAT) catalyzes the biosynthesis of S-adenosylmethionine (AdoMet). In mammals MAT activity derives from two separate genes which display a tissue-specific pattern of expression. While MAT1A is expressed only in the adult liver, MAT2A is expressed in non-hepatic tissues. The mechanisms behind the selective expression of these two genes are not fully understood. In the present report we have evaluated MAT1A and MAT2A methylation in liver and in other tissues, such as kidney, by methylation-sensitive restriction enzyme digestion of genomic DNA. Our data indicate that MAT1A is hypomethylated in liver and hypermethylated in non-expressing tissues. The opposite situ…

Altered expression of nonclassical HLA class Ib antigens in human renal cell carcinoma and its association with impaired immune response

2003

Abstract An optimal antitumoral immune response requires the activation of both CD8 + and CD4 + T lymphocytes by the peptide antigen presentation via the human leukocyte antigen (HLA) class I and class II molecules, respectively. Downregulation or loss of HLA molecules has been found in human renal cell carcinoma (RCC) and provides a strategy of these tumors to evade T-cell mediated immunosurveillance. In addition, a tumor-specific upregulation of HLA-G has been recently described in RCC, which also leads to an impaired immune response. We here summarize the frequency of the constitutive and/or interferon-γ (IFN-γ) inducible expression of nonclassical HLA class Ib antigens in RCC cell lines…

Characterization of a disulphide-bound Pir-cell wall protein (Pir-CWP) ofYarrowia lipolytica

2003

In this work we have studied the disulphide-bound group of cell wall mannoproteins of Yarrowia lipolytica and Candida albicans. In the case of Y. lipolytica, SDS-PAGE analysis of the beta-mercaptoethanol-extracted material from the purified cell walls of the yeast form, showed the presence of a main polypeptide of 45 kDa and some minor bands in the 100-200 kDa range. This pattern of bands is similar to that obtained in identical extracts in Saccharomyces cerevisiae (Moukadiri et al., 1999), and besides, all these bands cross-react with an antibody raised against beta-mercaptoethanol-extracted material from the purified cell walls of S. cerevisiae, suggesting that the 45 kDa band could be th…

An Anthropometric Analysis of Seated and Standing People

2012

Thermal radiative exchanges of the human body with surrounding surfaces play an essential role in describing the thermal conditions of people in a given environment. Moreover, they could be induced by, among other causes, the presence of high intensity radiant sources, like lighting spots or infrared heating panels. This implies that a suitable set of radiation data related to human body anthropometry is required. This set of data mainly comprises the body surface area, the clothing area factor, the effective radiation area factor, and projected area factors. Several analytical or experimental methods may be utilized in order to compute these parameters. A detailed description of the most c…

Survey of the bryophyte diversity of the Carnic Alps, including some interesting records for the Italian bryoflora

2010

Pratique juridique grecque et économie dans la Calabre post-byzantine (XIIe-XIIIe s.)

2014

Fonti recentemente edite conservate nell'Archivio Ducal de Medinaceli consentono di studiare sotto l'aspetto linguistico, sociale ed economico una zona della Calabria meridionale che nel regno normanno-svevo mantenne una popolazione di lingua rito e diritto privato bizantino. Recently published sources preserved in the Archivio Ducal de Medinaceli allow to study a linguistic, social and economic aspect of an area of ​​southern Calabria that in the Norman-Swabian kingdom maintained a population of rite and Byzantine private language. À partir des nouvelles sources, disponibles suite à la publication des actes privés grecs de l’Archivo Ducal de Medinaceli, on étudie ici la société rurale de l…