Search results for " Threshold"
showing 10 items of 497 documents
Automated analysis of images for molecular quantification in immunohistochemistry.
2018
The quantification of the expression of different molecules is a key question in both basic and applied sciences. While protein quantification through molecular techniques leads to the loss of spatial information and resolution, immunohistochemistry is usually associated with time-consuming image analysis and human bias. In addition, the scarce automatic software analysis is often proprietary and expensive and relies on a fixed threshold binarization. Here we describe and share a set of macros ready for automated fluorescence analysis of large batches of fixed tissue samples using FIJI/ImageJ. The quantification of the molecules of interest are based on an automatic threshold analysis of im…
Work places with stipend programme and its impact on poverty risk decrease: Case of Latvia
2012
The aim of the paper is to analyze theoretical concepts of poverty risk and to estimate the impact of the Workplaces with Stipend Programme (hereinafter WWS) on poverty risk decrease in Latvia. In order to assess the effects of the WWS three different surveys were carried out: of households with WWS participants, of experts in Latvian labour market issues, and of municipality representatives. The results were analyzed, together with analysis of statistical data. The paper concludes that in general the WWS has a very positive impact on WWS participants? household budgets (more than 95% of the WWS participants in all Latvian regions noted a significant impact of the WWS on their household bud…
Romanian Households Dealing with Precariousness: A life-course approach
2016
This paper addresses the main pathways through which households avoid slipping into poverty in Romania by employing a life-course approach. Recent researches on social stratification found that in every country we can delineate a particular social layer composed of households living just above the poverty threshold, whose members struggle to reach a more secure prosperity while facing constant threats of downward mobility. Drawing on recent precarious prosperity research, and based on in-depth interviews carried out in 2013 with 25 households situated in between poverty and prosperity from a Romanian city (Cluj-Napoca), we use a life-course approach in order to account for the main routes i…
No linealidad y asimetría en el proceso generador del Índice Ibex35
2013
This paper analyzes the behavior of Ibex35 from January 1999 to December 2001, in order to check if it follows a different process from random walk so its return is not a white noise and it can be predictable, against the efficient market hypothesis. For that, a nonlinear generating process of return will be considered and a STAR-APARCH model will be specified. This model allows a nonlinear behavior in the conditional mean and in the conditional variance. The empirical results show that the Ibex35 follows a nonlinear and asymmetric process, both in the conditional mean as in the conditional variance, so the weak-version of efficient market hypothesis is rejected. El trabajo analiza el compo…
Microbial environmental contamination in Italian dental clinics: A multicenter study yielding recommendations for standardized sampling methods and t…
2012
A microbiological environmental investigation was carried out in ten dental clinics in Italy. Microbial contamination of water, air and surfaces was assessed in each clinic during the five working days, for one week per month, for a three-month period. Water and surfaces were sampled before and after clinical activity; air was sampled before, after, and during clinical activity. A wide variation was found in microbial environmental contamination, both within the participating clinics and for the different sampling times. Before clinical activity, microbial water contamination in tap water reached 51,200cfu/mL (colony forming units per milliliter), and that in Dental Unit Water Systems (DUWS…
Skin innervation at different depths correlates with small fibre function but not with pain in neuropathic pain patients
2012
Background: Neuropathy can lead not only to impaired function but also to sensory sensitization. We aimed to link reduced skin nerve fibre density in different levels to layer-specific functional impairment in neuropathic pain patients and tried to identify pain-specific functional and structural markers. Methods: In 12 healthy controls and 36 patients with neuropathic pain, we assessed clinical characteristics, thermal thresholds (quantitative sensory testing) and electrically induced pain and axon reflex erythema. At the most painful sites and at intra-individual control sites, skin biopsies were taken and innervation densities in the different skin layers were assessed. Moreover, neurona…
Local Extinction of Dragonfly and Damselfly Populations in Low- and High-Quality Habitat Patches
2010
Understanding the risk of extinction of a single population is an important problem in both theoretical and applied ecology. Local extinction risk depends on several factors, including population size, demographic or environmental stochasticity, natural catastrophe, or the loss of genetic diversity. The probability of local extinction may also be higher in low-quality sink habitats than in high-quality source habitats. We tested this hypothesis by comparing local extinction rates of 15 species of Odonata (dragonflies and damselflies) between 1930-1975 and 1995-2003 in central Finland. Local extinction rates were higher in low-quality than in high-quality habitats. Nevertheless, for the thre…
Epidemic spreading and aging in temporal networks with memory
2018
Time-varying network topologies can deeply influence dynamical processes mediated by them. Memory effects in the pattern of interactions among individuals are also known to affect how diffusive and spreading phenomena take place. In this paper we analyze the combined effect of these two ingredients on epidemic dynamics on networks. We study the susceptible-infected-susceptible (SIS) and the susceptible-infected-removed (SIR) models on the recently introduced activity-driven networks with memory. By means of an activity-based mean-field approach we derive, in the long time limit, analytical predictions for the epidemic threshold as a function of the parameters describing the distribution of …
A heuristic fuzzy algorithm for assessing and managing tourism sustainability
2019
“Smartness” and “sustainability” are gaining growing attention from both practitioners and policy makers. “Smartness” and “sustainability” assessments are of crucial importance for directing, in a systemic perspective, the decision-making process toward sustainability and smart growth objectives. Sustainability assessment is a major challenge due to the multidisciplinary aspects involved that make the evaluation process complex and hinder the effectiveness of available monitoring tools. To achieve the assessment objective, we introduce an enhanced fuzzy logic-based framework for handling the inherent uncertainty and vagueness of the involved variables: we apply our approach to Italy, and we…
Alternative Polyadenylation Events Contribute to the Induction of NF-ATc in Effector T Cells
1999
Abstract The transcription factor NF-ATc is synthesized in three prominent isoforms. These differ in the length of their C terminal peptides and mode of synthesis. Due to a switch from the use of a 3′ polyA site to a more proximal polyA site, NF-ATc expression switches from the synthesis of the two longer isoforms in naive T cells to that of short isoform A in T effector cells. The relative low binding affinity of cleavage stimulation factor CstF-64 to the proximal polyA site seems to contribute to its neglect in naive T cells. These alternative polyadenylation events ensure the rapid accumulation of high concentrations of NF-ATc necessary to exceed critical threshold levels of NF-ATc for g…