Search results for " Variation"
showing 10 items of 1712 documents
Early gene m18, a novel player in the immune response to murine cytomegalovirus
2002
The identification of all antigenic peptides encoded by a pathogen, its T cell ‘immunome’, is a research aim for rational vaccine design. Screening of proteome-spanning peptide libraries or computational prediction is used to identify antigenic peptides recognized by CD8 T cells. Based on their high coding capacity, cytomegaloviruses (CMVs) could specify numerous antigenic peptides. Yet, current evidence indicates that the memory CD8 T cell response in a given haplotype is actually focused on a few viral proteins. CMVs actively interfere with antigen processing and presentation by the expression of immune evasion proteins. In the case of murine CMV (mCMV), these proteins are effectual in th…
Variability in the cadherin gene in an Ostrinia nubilalis strain selected for Cry1Ab resistance
2008
Transgenic corn expressing Cry1Ab (a Bacillus thuringiensis toxin) is highly effective in the control of Ostrinia nubilalis. For its toxic action, Cry1Ab has to bind to specific insect midgut proteins. To date, in three Lepidoptera species resistance to a Cry1A toxin has been conferred by mutations in cadherin, a protein of the Lepidoptera midgut membrane. The implication of cadherin in the resistance of an Ostrinia nubilalis colony (Europe-R) selected with Bacillus thuringiensis Cry1Ab protoxin was investigated. Several major mutations in the cadherin (cdh) gene were found, which introduced premature termination codons and/or large deletions (ranging from 1383 to 1701bp). The contribution …
Temperature dependence of spontaneous mutation rates.
2021
Mutation is the source of genetic variation and the fundament of evolution. Temperature has long been suggested to have a direct impact on realized spontaneous mutation rates. If mutation rates vary in response to environmental conditions, such as the variation of the ambient temperature through space and time, they should no longer be described as species-specific constants. By combining mutation accumulation with whole-genome sequencing in a multicellular organism, we provide empirical support to reject the null hypothesis of a constant, temperature-independent mutation rate. Instead, mutation rates depended on temperature in a U-shaped manner with increasing rates toward both temperature…
P58 Differential molecular diagnosis of uterine leiomyomas and leiomyosarcomas using DNA and RNA sequencing
2019
Introduction/Background Nowadays, the absence of standardized criteria to identify and differentiate uterine leiomyomas (LM) and leiomyosarcomas (LMS) prior to surgery, cause a significant stress in the patient, leading to unnecessary invasive procedures and additional costs to the National Health System. As consequence, the development of an accurate and non-invasive differential diagnostic methods in patients with surgical indication is needed to avoid the potential dissemination of hidden LMS from morcellation. We aim to identify differential genetic targets in LMS vs LM using Next Generation Sequencing to advance our knowledge in their differential diagnosis. Methodology A total of 13 L…
Influence of carbon to nitrogen ratio on nitrous oxide emission in an Integrated Fixed Film Activated Sludge Membrane BioReactor plant
2018
Abstract In this study a University of Cape Town (UCT) Integrated Fixed Film Activated Sludge (IFAS) Membrane BioReactor (MBR) wastewater treatment plant was monitored in terms of nitrous oxide (N2O) emissions. The short term effect on the N2O emission due to the influent carbon-to-nitrogen (C/N) ratio variation (C/N ratios of 2, 5 and 10 gCOD/gN) was evaluated. Since in a previous study, the effect of the C/N ratio was studied in the same system without biofilm (UCT-MBR configuration) the main aim here was to investigate the role of biofilms on N2O emissions. Under all the investigated C/N ratios, the N2O fluxes and the average emission factors were lower than that of previous studies with…
Lotus tenuis x L. corniculatus interspecific hybridization as a means to breed bloat-safe pastures and gain insight into the genetic control of proan…
2014
Background: Proanthocyanidins (PAs) are secondary metabolites that strongly affect plant quality traits. The concentration and the structure of these metabolites influence the palatability and nutritional value of forage legumes. Hence, modulating PAs in the leaves of forage legumes is of paramount relevance for forage breeders worldwide. The lack of genetic variation in the leaf PA trait within the most important forage species and the difficulties in engineering this pathway via the ectopic expression of regulatory genes, prompted us to pursue alternative strategies to enhance this trait in forage legumes of agronomic interest. The Lotus genus includes forage species which accumulate PAs …
Variabilidad en la utilización de los servicios de urgencias hospitalarios del Sistema Nacional de Salud
2010
ResumenObjetivoLos objetivos de este estudio fueron estimar las tasas de frecuentación a los servicios de urgencias hospitalarios (SUH) del Sistema Nacional de Salud (SNS) por áreas de salud, el porcentaje de ingresos, las razones estandarizadas de utilización de urgencias y analizar la relación con los recursos hospitalarios.MétodosEstudio ecológico combinando información de diversas fuentes (Encuesta de Establecimientos Sanitarios con Régimen de Internado 2006 y Conjunto Mínimo de Datos Básicos 2006) para estimar la frecuentación a los SUH y el porcentaje de ingresos asociado en 164 áreas de salud de 14 comunidades autónomas (CC.AA.).ResultadosLos 35,3 millones de habitantes de las 164 ár…
DISTRIBUTION AND CHARACTERIZATION OF MELANISM IN GRASS SNAKES FROM ITALY
2022
In snakes, melanism can improve thermoregulation efficiency, reproductive success and provide additional protection to internal organs from solar radiation. For these reasons, such conditions could be beneficial for animals living in both cold and warm environments. For widely distributed species of grass snake (genus Natrix), several single encounters of melanic individuals are reported in the literature. In the period 2015-2021 a significant number of unpublished observations of melanic Natrix helvetica and N. natrix were collected on the Italian territory and integrated with bibliographic data. The present work illustrates the chromatic variation within melanic individuals and investigat…
Morphological variation of the Oncocyclus irises (Iris: Iridaceae) in the southern Levant
2002
Morphological traits of Iris section Oncocyclus (Siems.) Baker in the southern Levant (Israel, Jordan, The Palestinian Authority and Sinai/Egypt) were analysed in order to clarify taxonomic relationships among taxa and the validity of diagnostic characters. Floral and vegetative characters were measured in 42 populations belonging to nine species during the peak of the flowering season in 1998‐2000. Pearson’s Coefficient of Racial Likelihood (CRL) was used to calculate morphological distances between populations. Twelve of the measured populations, distributed along the north-south aridity gradient in Israel, were further explored for morphological changes along the gradient. Cluster analys…
Defining the role of common variation in the genomic and biological architecture of adult human height
2014
Item does not contain fulltext Using genome-wide data from 253,288 individuals, we identified 697 variants at genome-wide significance that together explained one-fifth of the heritability for adult height. By testing different numbers of variants in independent studies, we show that the most strongly associated approximately 2,000, approximately 3,700 and approximately 9,500 SNPs explained approximately 21%, approximately 24% and approximately 29% of phenotypic variance. Furthermore, all common variants together captured 60% of heritability. The 697 variants clustered in 423 loci were enriched for genes, pathways and tissue types known to be involved in growth and together implicated genes…