Search results for " affinity"

Study of the morphological affinity between two species of benthic foraminifera from restricted environments: Rosalina douvillei (Cushman, 1928) and …

2020

A biometric comparison is carried out on selected tests of the foraminiferal species Rosalina douvillei (Cushman, 1928) and Trichohyalus aguayoi (Bermudez, 1935). The Rosalina douvillei specimens were recovered from the marginal marine Oligocene/Miocene site of Russingen (Mainz Basin, Germany), the lacustrine beds of the lower Miocene site of Bunol (Valencian Community, Spain) and the middle/ upper Miocene site of Fuendetodos (Aragon, Spain). The Trichohyalus aguayoi tests were collected in the current coastal lagoon of Torreblanca and recovered from Holocene cores sampled in the Peniscola marsh and in the coastal lagoon of l’Albufera de Valencia, all them in the Valencian Community (Spain)…

The oocyte derived growth factors, GDF9 and GDF9B, and their biological activities in 'in vitro' cell models

2007

Plato and Antisthenes in the Phaedo: A Reflexive Reading. Part One

2019

The purpose of this study is not so much to show the presence of Antisthenes in the dialogue, but rather to examine what Plato alludes to. The controversy over ideas between the two Socratics is historically very well-attested, as can already be seen in the Cratylus. Thus, it is reasonable to assume that this controversy must have affected Plato when he was writing a dialogue in which the importance of ideas and his new logic is undeniable. Hence, this paper will investigate the following question: what impact could Antisthenes’ denominative and definitory logic have on the equally denominative and definitory logic presented in the Phaedo given that the latter work in all probability preced…

Sulle orme del consumatore affinity analysis e knowledge visualization per il processo decisionale

2016

L’articolo analizza i reali comportamenti di acquisto dell’acquirente all’interno del punto vendita e individua i pattern latenti più significativi attraverso un’analisi di affinità di 128.364 scontrini estratti da un dataset di 715.662 transazioni effettuate in un anno. Lo studio individua le strutture associative più ricorrenti relativamente alla singola merceologia. Attraverso la tecnica della Market Basket Analysis è stato possibile individuare le categorie merceologiche che creano “poli d’attrazione” sul punto vendita stimolando acquisti di altre categorie. In particolar modo, applicando le regole associative sul punto vendita è stato possibile identificare le “aree calde” e le “aree f…

Immunoaffinity purification and characterization of mitochondrial membrane-bound D-3-hydroxybutyrate dehydrogenase from Jaculus orientalis.

2008

Abstract Background The interconversion of two important energy metabolites, 3-hydroxybutyrate and acetoacetate (the major ketone bodies), is catalyzed by D-3-hydroxybutyrate dehydrogenase (BDH1: EC 1.1.1.30), a NAD+-dependent enzyme. The eukaryotic enzyme is bound to the mitochondrial inner membrane and harbors a unique lecithin-dependent activity. Here, we report an advanced purification method of the mammalian BDH applied to the liver enzyme from jerboa (Jaculus orientalis), a hibernating rodent adapted to extreme diet and environmental conditions. Results Purifying BDH from jerboa liver overcomes its low specific activity in mitochondria for further biochemical characterization of the e…

The affinities of monoclonal antibodies against core antigen of hepatitis B virus

1994

Four monoclonal antibodies generated against the recombinant core antigen of hepatitis B virus are investigated for antigen binding. All exhibit a similar affinity to polystyrene-sorbed antigen but only one of them interacts with native form of HBcAg (an assembled particle) in solution. The presence of 0.1% sodium dodecylsulphate is required for the binding of other three antibodies. The phenomenon can be interpreted as inaccessibility of the corresponding epitopes unless the multimeric antigen structure is disrupted. The core antigen coated on polystyrene is considered as a similar exposed structure.

Search for autoantibodies to the human bradykinin B2 receptor.

1997

Screening for colorectal cancer with immunochemical faecal occult blood tests.

2012

Population-based studies have shown that guaiac faecal occult blood testing followed by colonoscopy in case of positivity can reduce colorectal cancer mortality. However these tests have been criticised for their fairly low sensitivity. For this reason attention has been given to alternative tests. The aim of this paper is to review the evidence for screening for colorectal cancer using qualitative immunochemical faecal occult blood tests. For the complete range of tested cut-off values, immunochemical faecal occult blood tests lead to higher diagnostic yield, improved sensitivity and greater participation. The optimal number of samples and the optimal cut-off value has to suit local resour…

Synthesis of biotin-labelled dexamethasone derivatives. Novel hormone-affinity probes.

1983

A new, general methodology for 'sandwich' affinity chromatography of steroid hormone receptors is proposed, the part purification of the human spleen tumor glucocorticoid receptor is quoted as an illustration. 9-Fluoro-16 alpha-methyl-11 beta, 17-dihydroxy-1,4-androstadiene-3-one-17 beta-carboxylic acid was coupled to biotin using pentamethylenediamine (BioDex 1) as a spacer. The bifunctional derivative binds to glucocorticoid receptors and avidin-Sepharose and efficiently protects the glucocorticoid receptor against inactivation when previously added during homogenisation. We have standardized the capacity and optimum conditions for elution of receptor-BioDex-1 complexes which are bound to…

Gas-phase ion chemistry of protonated melatonin

2005

The gas-phase ion chemistry of protonated melatonin has been investigated by mass spectrometric (MS) techniques involving chemical ionisation, fast atom bombardment and electrospray ionisation. Either naturally- occurring or collision-induced decomposition e-D derivatives obtained by exchange with D(2)O. The analysis of experimental results allows definite pathways for the formation of the ion at m/z 174 to be assigned and sheds some more light on the overall fragmentation pathways. Experiments on labelled derivatives evidenced H-D scrambling processes during fragmentation