Search results for " anatomia"
showing 10 items of 1072 documents
Therapeutic afucosylated monoclonal antibody and bispecific T-cell engagers for T-cell acute lymphoblastic leukemia
2021
BackgroundT-cell acute lymphoblastic leukemia (T-ALL) is an aggressive disease with a poor cure rate for relapsed/resistant patients. Due to the lack of T-cell restricted targetable antigens, effective immune-therapeutics are not presently available and the treatment of chemo-refractory T-ALL is still an unmet clinical need. To develop novel immune-therapy for T-ALL, we generated an afucosylated monoclonal antibody (mAb) (ahuUMG1) and two different bispecific T-cell engagers (BTCEs) against UMG1, a unique CD43-epitope highly and selectively expressed by T-ALL cells from pediatric and adult patients.MethodsUMG1 expression was assessed by immunohistochemistry (IHC) on a wide panel of normal t…
P-selectin glycoprotein ligand-1 as a potential target for humoral immunotherapy of multiple myeloma.
2008
Monoclonal antibodies (mAbs), successfully adopted in the treatment of several haematological malignancies, have proved almost ineffective in multiple myeloma (MM), because of the lack of an appropriate antigen for targeting and killing MM cells. Here, we demonstrate that PSGL1, the major ligand of P-Selectin, a marker of plasmacytic differentiation expressed at high levels on normal and neoplastic plasma cells, may represent a novel target for mAb-mediated MM immunotherapy. The primary effectors of mAb-induced cell-death, complement-mediated lysis (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC), were investigated using U266B1 and LP1 cell-lines as models. Along with immunolo…
DNA IN FORENSIC GENETICS
2008
Forensic molecular genetics has been evolved from a rapidly developing field into accepted forensic science, leading DNA databases with DNA profiles from suspects and convicted offenders. DNA evidence has taken a central role by carrying a significant weight for convictions in the court of justice, as well as by excluding innocent suspects early on in a criminal investigation. At present, a robust system for DNA typing has been established for paternity testing and forensic stain analysis in criminal casework. In addition, forensic biologist should be aware about the ethical and legal consequences of their research on the society and the criminal justice system. New technologies will only b…
DNA TYPING OF BLOODSTAIN: A CASEWORK OF MORTAL CRASH
2008
Since 1997 the analysis of DNA recovered from handled objects, or trace DNA has become routine and is frequently demanded from crime scene examination. However, this analysis often produces unpredictable results. The factor affecting the recovery of full profiles are numerous, and as demonstrated in casework and research, retrieving useable profiles from trace DNA exhibits is not guaranteed. There are numerous factors that affect the rate of recovery of full profiles, such as the ability of the individual to shed cells, the substrate surface, the time of contact and delay until analysis, and the environment. In addition to these external conditions, there are many varying methods used to co…
DNA typing from vaginal smears slides and stained sperm-positive evidence in suspected rape cases.
2009
Rape is a largely un-witnessed crime, and may be difficult to obtained statements from the victim, since most of the victims suffer from emotional damage as well as physical injury. The physical examination by forensic specialist is extremely important.
Cisplatin-induced endoreduplication in CHO cells: DNA damage and inhibition of topoisomerase II.
2006
It has been proposed that polyploid cells that arise during a variety of pathological conditions and as a result of exposure to genotoxicants, typically in the liver, become aneuploid through genetic instability. Aneuploidy contributes to, or even drives, tumour development. We have assessed the capacity of the drug cisplatin, one of the most commonly used compounds for the treatment of malignancies, to induce endoreduplication, a particular type of polyploidy, in cultured Chinese hamster AA8 cells. Taking into account that any interference with DNA topoisomerase II (topo II) function leads to endoreduplication, we have found that treatment of the cells with this platinum compound results i…
The Histone Deacetylase Inhibitor JAHA Down-Regulates pERK and Global DNA Methylation in MDA-MB231 Breast Cancer Cells
2015
The histone deacetylase inhibitor N-1-(ferrocenyl)-N-8-hydroxyoctanediamide (JAHA) down-regulates extracellular-signal-regulated kinase (ERK) and its activated form in triple-negative MDA-MB231 breast cancer cells after 18 h and up to 30 h of treatment, and to a lesser extent AKT and phospho-AKT after 30 h and up to 48 h of treatment. Also, DNA methyltransferase 1 (DNMT1), 3b and, to a lesser extent, 3a, downstream ERK targets, were down-regulated already at 18 h with an increase up to 48 h of exposure. Methylation-sensitive restriction arbitrarily-primed (MeSAP) polymerase chain reaction (PCR) analysis confirmed the ability of JAHA to induce genome-wide DNA hypomethylation at 48 h of expos…
Decorin transfection induces proteomic and phenotypic modulation in breast cancer cells 8701-BC
2008
Decorin is a prototype member of the small leucine-rich proteoglycan family widely distributed in the extracellular matrices of many connective tissues, where it has been shown to play multiple important roles in the matrix assembly process, as well as in some cellular activities. A major interest for decorin function concerns its role in tumorigenesis, as growth-inhibitor of different neoplastic cells, and potential antimetastatic agent. The aim of our research was to investigate wide-ranged effects of transgenic decorin on breast cancer cells. To this purpose we utilized the well-characterized 8701-BC cell line, isolated from a ductal infiltrating carcinoma of the breast, and two derived …
Le funzioni delle reti mirabili encefaliche
2009
I numerosi spiaggiamenti di delfini e balenottere potrebbero collegarsi a fenomeni d’ipossia cerebrale causata dalla vasodilatazione in vaste aree delle reti mirabili encefaliche. Questa vasodilatazione riduce il controllo su di CBF e MAP e non è compensata da un apporto aggiuntivo diretto di sangue come avviene per il Gatto, ruminanti, Giraffa ed altri mammiferi provvisti di reti mirabili encefaliche. La pressione intracranica sarebbe l’effettiva pressione di flusso della circolazione cerebrale. Studi su altri organi hanno invece trovato che l’effettiva pressione di flusso è data dalla pressione critica di chiusura (storno), localizzata nelle arteriole. Weyland A. et all.(2000), affermano …
Mononuclear Perfluoroalkyl-Heterocyclic Complexes of Pd(II): Synthesis, Structural Characterization and Antimicrobial Activity
2020
Two mononuclear Pd(II) complexes [PdCl2(pfptp)] (1) and [PdCl2(pfhtp)] (2), with ligands 2-(3-perfluoropropyl-1-methyl-1,2,4-triazole-5yl)-pyridine (pfptp) and 2-(3-perfluoroheptyl-1-methyl-1,2,4-triazole-5yl)-pyridine (pfhtp), were synthesized and structurally characterized. The two complexes showed a bidentate coordination of the ligand occurring through N atom of pyridine ring and N4 atom of 1,2,4-triazole. Both complexes showed antimicrobial activity when tested against both Gram-negative and Gram-positive bacterial strains.