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showing 10 items of 6903 documents

A genomic reappraisal of symbiotic function in the aphid/Buchnera symbiosis: reduced transporter sets and variable membrane organisations.

2011

International audience; Buchnera aphidicola is an obligate symbiotic bacterium that sustains the physiology of aphids by complementing their exclusive phloem sap diet. In this study, we reappraised the transport function of different Buchnera strains, from the aphids Acyrthosiphon pisum, Schizaphis graminum, Baizongia pistaciae and Cinara cedri, using the re-annotation of their transmembrane proteins coupled with an exploration of their metabolic networks. Although metabolic analyses revealed high interdependencies between the host and the bacteria, we demonstrate here that transport in Buchnera is assured by low transporter diversity, when compared to free-living bacteria, being mostly bas…

multidisciplinary scienceslcsh:MedicinePlant ScienceinterdépendanceBiochemistryTransmembrane Transport ProteinsBacterial Physiologylcsh:ScienceIntegral membrane proteinGeneticsbactérie0303 health sciencesPlant PestsMultidisciplinaryMicroscopy ConfocalbiologyMembrane transport protein030302 biochemistry & molecular biologybuchnera aphidicolamicroscopy confocalGenomicsHydrogen-Ion ConcentrationTransmembrane proteinFunctional GenomicsBiochemistrysymbiosis geneticsMetabolic PathwaysMetabolic Networks and PathwaysResearch Articlecell membrane ultrastructurescience and technologyMicrobiology03 medical and health sciencesMetabolic NetworksBuchneraAnimalsSymbiosisBiology030304 developmental biologyObligateCell Membranelcsh:RProteinsComputational BiologyMembrane Transport ProteinsBiological TransportBacteriologyPlant Pathologybiochemical phenomena metabolism and nutritionbiology.organism_classificationAcyrthosiphon pisumTransmembrane ProteinsaphidsMESH: SymbioseMetabolismMembrane proteinGenes Bacterialbiology.proteinlcsh:QBuchnerabuchnera aphidicola;aphids;microscopy confocal;symbiosis genetics;cell membrane ultrastructure;multidisciplinary sciences;science and technologyFunction (biology)[SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/SymbiosisPLoS ONE
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Cardiolipin content controls mitochondrial coupling and energetic efficiency in muscle

2020

Decreasing mitochondrial energy-production efficiency in skeletal muscle can confer protection against diet-induced obesity.

muscle[SDV]Life Sciences [q-bio]Respiratory chainDiseases and DisordersOxidative phosphorylation[SDV.BC]Life Sciences [q-bio]/Cellular Biology030204 cardiovascular system & hematology03 medical and health scienceschemistry.chemical_compound0302 clinical medicinemedicineCardiolipin[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyInner mitochondrial membrane[SDV.BC] Life Sciences [q-bio]/Cellular BiologyResearch ArticlesFatty acid synthesisComputingMilieux_MISCELLANEOUS030304 developmental biology2. Zero hungerchemistry.chemical_classification0303 health sciencesMultidisciplinary[SDV.MHEP] Life Sciences [q-bio]/Human health and pathologyATP synthasebiologyfungifood and beveragesSciAdv r-articlesSkeletal muscleFatty acidCell BiologymitochondrialCell biologymedicine.anatomical_structurechemistryCardiolipinbiology.protein[SDV.MHEP]Life Sciences [q-bio]/Human health and pathologyResearch Article
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Dairy calcium intake and lifestyle risk factors for bone loss in hiv-infected and uninfected mediterranean subjects

2012

Abstract Background Despite the reported high prevalence of osteoporosis in the human immunodeficiency virus (HIV)-population, there have been no previous studies examining dairy calcium intake and bone mineral density (BMD) in HIV-subjects. We assessed the prevalence of low BMD in HIV-infected and uninfected subjects and analyzed the effects of calcium intake, lifestyle and HIV-related risk factors on BMD. Methods One hundred and twelve HIV-infected subjects were consecutively enrolled. Seventy- six HIV-uninfected subjects matched for age and sex were enrolled as the control group. The HIV-subjects were interviewed about lifestyle habits and completed a weekly food-frequency questionnaire …

musculoskeletal diseasesAdultMalemedicine.medical_specialtyFRAXSettore MED/09 - Medicina InternaBone densitySettore MED/17 - Malattie InfettiveOsteoporosisHIV Infectionslcsh:Infectious and parasitic diseasesBone remodelingAbsorptiometry PhotonBone DensityRisk FactorsSurveys and QuestionnairesInternal medicineVitamin D and neurologyBone mineral densityHumansMedicinelcsh:RC109-216Life StyleBone mineralMediterranean Regionbusiness.industryOsteopeniaCase-control studyOsteoporosiHIVMiddle AgedDairy intakemedicine.diseaseDietOsteopeniaInfectious DiseasesEndocrinologyCase-Control StudiesOsteoporosisCalciumFemalebusinessSettore MED/36 - Diagnostica Per Immagini E RadioterapiaResearch Article
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Abnormalities in alpha-dystroglycan expression in MDC1C and LGMD2I muscular dystrophies

2004

We recently identified mutations in the fukutin related protein (FKRP) gene in patients with congenital muscular dystrophy type 1C (MDC1C) and limb girdle muscular dystrophy type 2I (LGMD2I). The sarcolemma of these patients typically displays an immunocytochemical reduction of alpha-dystroglycan. In this report we extend these observations and report a clear correlation between the residual expression of alpha-dystroglycan and the phenotype. Three broad categories were identified. Patients at the severe end of the clinical spectrum (MDC1C) were compound heterozygote between a null allele and a missense mutation or carried two missense mutations and displayed a profound depletion of alpha-d…

musculoskeletal diseasesAdultPathologymedicine.medical_specialtyNonsense mutationBlotting WesternDNA Mutational AnalysisMedizinCompound heterozygosityPolymerase Chain ReactionMuscular DystrophiesPathology and Forensic MedicineFetusDystroglycanmedicineMissense mutationHumansPentosyltransferasesMuscular dystrophyChildDystroglycansMuscle SkeletalGeneticsFukutin-related proteinMembrane GlycoproteinsbiologyProteinsmedicine.diseasemusculoskeletal systemImmunohistochemistryCytoskeletal ProteinsPhenotypeMutationbiology.proteinCongenital muscular dystrophyLimb-girdle muscular dystrophyRegular Articles
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Reliability and methodology of quantitative assessment of harvested and unharvested patellar tendons of ACL injured athletes using ultrasound tissue …

2019

Ultrasound tissue characterization (UTC) imaging has been previously used to describe the characteristics of patellar and Achilles tendons. UTC imaging compares and correlates successive ultrasonographic transverse tendon images to calculate the distribution of four color-coded echo-types that represent different tendon tissue types. However, UTC has not been used to describe the characteristics of patellar tendons after anterior cruciate ligament reconstruction (ACLR). The aim of this cross-sectional study was to assess the intra and inter-rater reliability of the UTC in unharvested and harvested patellar tendons of patients undergoing ACLR. Intra and inter-rater reliability of both UTC da…

musculoskeletal diseasesAllied health and rehabilitation scienceEcho-types distributionTendon qualityanterior cruciate ligamentpolvilumpioecho-types distributionmusculoskeletal systemtendon qualitykudoksetGraftgraftultraäänitutkimuslcsh:Sports medicineAnterior cruciate ligamentlcsh:RC1200-1245Research ArticleUTC
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Sense and Antisense DMPK RNA Foci Accumulate in DM1 Tissues during Development.

2015

International audience; Myotonic dystrophy type 1 (DM1) is caused by an unstable expanded CTG repeat located within the DMPK gene 3'UTR. The nature, severity and age at onset of DM1 symptoms are very variable in patients. Different forms of the disease are described, among which the congenital form (CDM) is the most severe. Molecular mechanisms of DM1 are well characterized for the adult form and involve accumulation of mutant DMPK RNA forming foci in the nucleus. These RNA foci sequester proteins from the MBNL family and deregulate CELF proteins. These proteins are involved in many cellular mechanisms such as alternative splicing, transcriptional, translational and post-translational regul…

musculoskeletal diseasesCCAAT-Enhancer-Binding Protein-deltacongenital hereditary and neonatal diseases and abnormalities[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiologylcsh:MedicineMice Transgenic[SDV.GEN.GH] Life Sciences [q-bio]/Genetics/Human genetics[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyMyotonin-Protein KinaseMice[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]AnimalsHumansMyotonic DystrophyRNA AntisenseRNA Messengerlcsh:ScienceMuscle SkeletalCell NucleusMyocardiumlcsh:R[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/NeurobiologyBrainGene Expression Regulation DevelopmentalRNA-Binding Proteins[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyEmbryo MammalianAlternative SplicingDisease Models Animal[SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human geneticsAnimals Newborn[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]lcsh:QTrinucleotide Repeat ExpansionSignal TransductionResearch ArticlePloS one
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Bone and cartilage characteristics in postmenopausal women with mild knee radiographic osteoarthritis and those without radiographic osteoarthritis

2015

Objectives: To evaluate the association between radiographically-assessed knee osteoarthritis and femoral neck bone characteristics in women with mild knee radiographic osteoarthritis and those without radiographic osteoarthritis. Methods: Ninety postmenopausal women (mean age [SD], 58 [4] years; height, 163 [6] cm; weight, 71 [11] kg) participated in this cross-sectional study. The severity of radiographic knee osteoarthritis was defined using Kellgren-Lawrence grades 0=normal (n=12), 1=doubtful (n=25) or 2=minimal (n=53). Femoral neck bone mineral content (BMC), section modulus (Z), and cross-sectional area (CSA) were measured with DXA. The biochemical composition of ipsilateral knee cart…

musculoskeletal diseasesKnee JointFemur NeckQuantitative MRIMiddle AgedOsteoarthritis Kneemusculoskeletal systemBone StrengthBone and BonesPostmenopausal WomenPostmenopauseAbsorptiometry PhotonCartilageCross-Sectional StudiesBone DensityKellgren and Lawrence GradeOsteoarthritisHumansOsteoporosisFemaleOriginal Articlehuman activitiesAged
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Ultrasound-guided percutaneous treatment of a calcific acromioclavicular joint

2020

Abstract Rationale: Calcific tendinopathy is one of the most frequent causes of nontraumatic shoulder pain. However, intra-articular calcifications appear to be an infrequent condition. We herein report a rare case study of an intra-articular calcification of the acromioclavicular joint. Patient concerns: A 46-year-old man presented with an acute pain in the anterior superior region of the left shoulder which also radiated to the left cervical region. The man during the physical evaluation also presented severe functional limitation of the shoulder movements in all planes of motion. Diagnoses: The diagnosis was carried out through a radiographic and an echotomographic examination, highlight…

musculoskeletal diseasesMaleacromioclavicular joint ;ultrasound-guided percutaneous treatmentshoulderCalcinosisMiddle Aged6300Acromioclavicular JointTendinopathyHumanspainClinical Case ReportUltrasonography InterventionalResearch ArticleMedicine
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MRI evaluation to predict tendon size for knee ligament reconstruction.

2017

Background The aim of this study is to evaluate a possible correlation between specific anthropometric parameters and sizes of knee tendons commonly used for ACL reconstruction. We hypothesized that specific clinical and radiological knee measurements could be better tendon sizes predictors than age, gender, height and weight. Materials and methods 100 consecutive patients were enrolled and 77 patients met the inclusion criteria of the study. All patients underwent a MRI of the knee with a 1.5 T super conducting MR System. For each patient, anthropometric data such as gender, height, weight, body mass index (BMI) and knee circumference were recorded. Specific MRI knee measurements were perf…

musculoskeletal diseasesOrthodontics030222 orthopedicsbusiness.industry05 social sciences050801 communication & media studiesmusculoskeletal systemCircumferenceTendon03 medical and health sciences0508 media and communications0302 clinical medicinemedicine.anatomical_structureGracilis tendonKnee ligamentquadriceps tendon autograft patellar tendon autograft acl reconstruction mri graft size measurement preoperative measures hamstringmedicineOrthopedics and Sports MedicinePatellaOriginal ArticleQuadriceps tendonbusinessBody mass indexHamstringMuscles, ligaments and tendons journal
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Identification of the Yersinia enterocolitica urease beta subunit as a target antigen for human synovial T lymphocytes in reactive arthritis.

1993

The local T-cell response to bacterial antigens is involved in the pathogenesis of reactive arthritis (ReA). Here, we have identified a 19-kDa antigen of Yersinia enterocolitica O:9 recognized by Yersinia-specific synovial fluid CD4+ T cells in two patients with Yersinia-induced ReA. N-terminal amino acid sequencing of this protein revealed that it was identical to the 19-kDa urease beta subunit of Y. enterocolitica O:9. This protein has previously been shown to be arthritogenic in preimmunized rats after intra-articular injection. Analysis of the T-cell response to this protein showed that it contains several T-cell epitopes, one of which cross-reacts with other enterobacteria not able to …

musculoskeletal diseasesProtein subunitT-LymphocytesImmunologyMolecular Sequence DataBiologyLymphocyte ActivationMicrobiologyEpitopeMicrobiologyAntigenProhibitinsSynovial FluidSynovial fluidHumansAmino Acid SequenceYersinia enterocoliticaHLA-DR AntigenYersinia enterocoliticaAntigens BacterialSequence Homology Amino AcidArthritisT lymphocyteHLA-DR Antigensbiology.organism_classificationbacterial infections and mycosesUreaseInfectious DiseasesParasitologyBacterial antigenResearch Article
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