Search results for " assembly."

Dynamic remodeling of histone modifications in response to osmotic stress in Saccharomyces cerevisiae.

2014

Abstract Background Specific histone modifications play important roles in chromatin functions; i.e., activation or repression of gene transcription. This participation must occur as a dynamic process. Nevertheless, most of the histone modification maps reported to date provide only static pictures that link certain modifications with active or silenced states. This study, however, focuses on the global histone modification variation that occurs in response to the transcriptional reprogramming produced by a physiological perturbation in yeast. Results We did a genome-wide chromatin immunoprecipitation analysis for eight specific histone modifications before and after saline stress. The most…

Advanced Monitoring of Rotor Broken Bar in Double Squirrel Cage Induction Machines Based on Wavelet Analysis

2012

The diagnosis of induction machine faults is commonly realized through Motor Current Signature Analysis (MCSA), i.e. by classical spectrum analysis of the input currents. In case of large or double cage induction motors, rotor broken bars fault detection based on sideband current components may fail due to the presence of inter bar currents that reduce the degree of rotor asymmetry, leading to reduced relevance of these spectral components. But inter bar currents produce core vibrations in the axial direction, which can be detected using vibration analysis techniques, in order to overcome the limits of the classical MCSA for these purposes. In this paper, an advanced use of the Discrete wav…

YK:n legitimiteetti yleiskokouksen kautta tarkasteltuna

2022

Legitimiteetti tai legitiimisyys on yksi Yhdistyneiden kansakuntien (YK) toiminnan kannalta keskeisistä käsitteistä, joka linkittyy myös monenkeskiseen yhteistyöhön ja siihen sitoutumiseen. Tässä katsausartikkelissa tarkastellaan YK:n legitimiteettiä yleiskokouksen näkökulmasta ja kysytään, miten yleiskokouksen uudistamista koskeva keskustelu vaikuttaa puolestaan koko YK:n legitimiteettiin. Erityisenä kiinnostuksen kohteena on kirjallisuudessa vähälle huomiolle jäänyt YK:n yleiskokous kansainvälisen politiikan toimijana ja yleiskokouksen mandaatti toimia eri kansainvälisen politiikan alaan kuuluvissa kysymyksissä. Artikkeli osoittaa, miten yleiskokouksella on tärkeä tehtävä koko YK:n kannal…

PIL.20-21-Unit 7. The role of the United Nations

2020

Public International Law. Unit 7. The role of the United Nations. Powerpoint of the Academic course 2020-2021.

Low-temperature molecular layer deposition using monifunctional aromatic precursors and ozone-based ring-opening reactions

2017

Molecular layer deposition (MLD) is an increasingly used deposition technique for producing thin coatings consisting of purely organic or hybrid inorganic-organic materials. When organic materials are prepared, low deposition temperatures are often required to avoid decomposition, thus causing problems with low vapor pressure precursors. Monofunctional compounds have higher vapor pressures than traditional bi- or trifunctional MLD precursors, but do not offer the required functional groups for continuing the MLD growth in subsequent deposition cycles. In this study, we have used high vapor pressure monofunctional aromatic precursors in combination with ozone-triggered ring-opening reactions…

Purified Membrane-Containing Procapsids of Bacteriophage PRD1 Package the Viral Genome

2009

Icosahedral-tailed double-stranded DNA (dsDNA) bacteriophages and herpesviruses translocate viral DNA into a preformed procapsid in an ATP-driven reaction by a packaging complex that operates at a portal vertex. A similar packaging system operates in the tailless dsDNA phage PRD1 (Tectiviridae family), except that there is an internal membrane vesicle in the procapsid. The unit-length linear dsDNA genome with covalently linked 5'-terminal proteins enters the procapsid through a unique vertex. Two small integral membrane proteins, P20 and P22, provide a conduit for DNA translocation. The packaging machinery also contains the packaging ATPase P9 and the packaging efficiency factor P6. Here we…

Fragmentation and Coverage Variation in Viral Metagenome Assemblies, and Their Effect in Diversity Calculations

2015

Metagenomic libraries consist of DNA fragments from diverse species, with varying genome size and abundance. High-throughput sequencing platforms produce large volumes of reads from these libraries, which may be assembled into contigs, ideally resembling the original larger genomic sequences. The uneven species distribution, along with the stochasticity in sample processing and sequencing bias, impacts the success of accurate sequence assembly. Several assemblers enable the processing of viral metagenomic data de novo, generally using overlap layout consensus or de Bruijn graph approaches for contig assembly. The success of viral genomic reconstruction in these datasets is limited by the de…

Replication of hepatitis C virus

2000

The evolution of collective infectious units in viruses

2019

Viruses frequently spread among cells or hosts in groups, with multiple viral genomes inside the same infectious unit. These collective infectious units can consist of multiple viral genomes inside the same virion, or multiple virions inside a larger structure such as a vesicle. Collective infectious units deliver multiple viral genomes to the same cell simultaneously, which can have important implications for viral pathogenesis, antiviral resistance, and social evolution. However, little is known about why some viruses transmit in collective infectious units, whereas others do not. We used a simple evolutionary approach to model the potential costs and benefits of transmitting in a collect…

De novogenome assembly of the land snailCandidula unifasciata(Mollusca: Gastropoda)

2021

AbstractAmong all molluscs, land snails are an economically and scientifically interesting group comprising edible species, alien species and agricultural pests. Yet, despite its high diversity, the number of whole genomes publicly available is still scarce. Here, we present the draft genome assembly of the land snailCandidula unifasciata, a widely distributed species along central Europe, which belongs to Geomitridae family, a group highly diversified in the Western-Palearctic region. We performed a whole genome sequencing, assembly and annotation of an adult specimen based on PacBio and Oxford Nanopore long read sequences as well as Illumina data. A genome of about 1.29 Gb was generated w…