Search results for " border"
showing 10 items of 142 documents
Screening for Bacillus thuringiensis Crystal Proteins Active against the Cabbage Looper, Trichoplusia ni
2000
Abstract Toxicity tests were performed to find among Cry1 and Cry2 Bacillus thuringiensis crystal proteins those with high activity against the cabbage looper. Tests were performed with neonate larvae on surface-contaminated artificial diet. The crystal proteins found to be toxic were, from higher to lower toxicity: Cry1Ac, Cry1Ab, Cry1C, Cry2Aa, Cry1J, and Cry1F (LC50 of 1.1–4.1, 3.4–4.4, 12, 34, 87, and 250 ng/cm2, respectively). Cry1B, Cry1D, and Cry1E can be considered nontoxic (LC50 higher than 2500 ng/cm2). Cry1Aa was moderately toxic to nontoxic, depending on the source (LC50 of 420 ng/cm2 from PGS and 8100 ng/cm2 from Ecogen). In vitro binding assays with trypsin-activated 125I-labe…
Overproduction of the Bacillus thuringiensis Vip3Aa16 toxin and study of its insecticidal activity against the carob moth Ectomyelois ceratoniae
2015
Abstract The vip3Aa16 gene of Bacillus thuringiensis strain BUPM95 was cloned and expressed in Escherichia coli . Optimization of Vip3A16 protein expression was conducted using Plackett–Burman design and response surface methodology. Accordingly, the optimum Vip3A16 toxin production was 170 μg/ml at 18 h post-induction time and 39 °C post-induction temperature. This corresponds to an improvement of 21 times compared to the starting conditions. The insecticidal activity, evaluated against Ectomyelois ceratoniae , displayed an LC 50 value of 40 ng/cm 2 and the midgut histopathology of Vip3Aa16 fed larvae showed vacuolization of the cytoplasm, brush border membrane destruction, vesicle formati…
A geographical note about the journey
2022
This paper summarizes the contents of the in print monographic volume entitled About the journey. Stasis, chiasmus and interruptions. A geography essay. It aims at being part of the epistemological debate on the role of the journey intended as a complex action through which relationships between man and space are strengthened and the fundamental cognitive processes are activated. In the first chapter, we want to foster a consideration on the tripartite scan that has the journey as the terminal outcome: stasis, trigger, experience. The paper has its roots in the international and national debates of New Cultural Geography, an essential theoretical prerequisite for setting up a theoretically …
About the journey. Stasis, chiasmus and interruptions A geography essay
2022
The journey is a very stimulating function to read the congeries of relationships that intersect and signify the bonds of men with places. A device that activates, in Geography, different heuristic perspectives. The first chapter then aims at being part of the epistemological debate on the function of journey intended as a complex action through which relationships between man and space are strengthened and the fundamental cognitive processes are activated. In particular, in the first chapter, we want to foster a consideration on the tripartite scan that has the terminal outcome in the journey: stasis, trigger, experience. The second chapter then follows some literary suggestions to try to …
Solitary keratoacanthoma involving upper lip: a diagnostic dilemma - case report and a brief review
2010
Keratoacanthoma (KA) is a benign epithelial proliferative lesion which frequently occurs on the sun exposed areas of the skin. KA originates within the pilosebaceous apparatus of the skin and may be solitary or multiple. Solitary KA can be difficult to differentiate from squamous cell carcinoma (SCC) both clinically and microscopically. However, the clinical course of the lesion and its ability to self involute makes it a distinct clinical entity. Solitary KA appears on the vermilion border of the lower lips with some frequency. A case of keratoacanthoma involving the upper lip is reported presenting as an exophytic growth that resolved after excisional biopsy. Photo- graphic documentation …
Historia i mitologia Kresów Wschodnich Rzeczypospolitej
2020
Celem artykułu jest ukazanie dorobku historiograficznego na temat Kresów Wschodnich Rzeczypospolitej na przestrzeni ostatnich trzydziestu lat, kiedy po usunięciu cenzury wielu badaczy w polskich środowiskach akademickich oraz wielu literatów, publicystów, dokumentalistów, autorów wspomnień i pamiętników, poczęło publikować ważne prace naukowe, popularnonaukowe i publicystyczne. W artykule przedstawiony jest dorobek najwybitniejszych i najpłodniejszych badaczy Kresów. Autor poddaje analizie najważniejsze wypowiedzi i dzieła m.in. prof. Jacka Kolbuszewskiego, Bolesława Hadaczka, Janusza Tazbira, Jerzego Janickiego, Witolda Szolgini, Tadeusza Olszańskiego, Romana Aftanazego, Daniela Beauvois o…
Monitoring of Bacillus Thuringiensis Cry3Aa Toxin Pore Formation using Artificial Bilayer Array with Fused Brush Border Membrane Vesicles from Colora…
2017
Study of the bacillus thuringiensis Cry1Ia protein oligomerization promoted by midgut brush border membrane vesicles of lepidopteran and coleopteran …
2020
Bacillus thuringiensis (Bt) produces insecticidal proteins that are either secreted during the vegetative growth phase or accumulated in the crystal inclusions (Cry proteins) in the stationary phase. Cry1I proteins share the three domain (3D) structure typical of crystal proteins but are secreted to the media early in the stationary growth phase. In the generally accepted mode of action of 3D Cry proteins (sequential binding model), the formation of an oligomer (tetramer) has been described as a major step, necessary for pore formation and subsequent toxicity. To know if this could be extended to Cry1I proteins, the formation of Cry1Ia oligomers was studied by Western blot, after the incuba…
Identità russe in transito nella New York di Ljudmila Ulickaja
2021
The essay analyses the atypical concept of border in the povest' by Ljudmila Ulickaja 'Veselye pochorony' (1997). This short novel offers several images related to the symbolic setting represented mainly by the city of New York, which embodies an idealized 'border city', where some Russians immigrants arrive and meet in 1991. First of all my research aims to to examine the role played by a few peculiar places represented in 'Veselye pochorony', in order to highlight the emotional dynamics of some Ulickaja's characters, who have to face the difficulties of integration in a cultural and social environment, totally different from the one they come from. Moreover this essay investigates the dev…
Między Starą a Nową Ojczyzną – muzyczna aktywność cymbalistów na Dolnym Śląsku w latach 50. XX wieku
2022
W artykule omówiono zagadnienie muzycznej aktywności cymbalistów, którzy zmuszeni byli do migracji z przedwojennych kresów wschodnich (Wileńszczyzny, Galicji Wschodniej) na Dolny Śląsk po II wojnie światowej. Będąc nowymi osadnikami na tzw. Ziemiach Odzyskanych, otoczeni ludnością o różnym pochodzeniu regionalnym, cymbaliści próbowali w nowych warunkach kontynuować muzyczną działalność. W tekście ukazane są różne sposoby adaptacji do nowej sytuacji wykonawczej w nowym miejscu zamieszkania: budowanie kolejnych instrumentów, granie w kapelach ludowych składających się z członków o różnym pochodzeniu regionalnym, próby indywidualnego muzykowania oparte na wykonywaniu muzyki tanecznej z regionu…