Search results for " culture"
showing 10 items of 5779 documents
Integrated signaling network involving calcium, nitric oxide, active oxygen species but not mitogen-activated protein kinases in BcPG1-elicited grape…
2006
We have already reported the identification of the endopolygalacturonase 1 (BcPG1) from Botrytis cinerea as a potent elicitor of defense responses in grapevine, independently of its enzymatic activity. The aim of the present study is the analysis of the signaling pathways triggered by BcPG1 in grapevine cells. Our data indicate that BcPG1 induces a Ca2+ entry from the apoplasm, which triggers a phosphorylation-dependent nitric oxide (NO) production via an enzyme probably related to a NO synthase. Then NO is involved in i) cytosolic calcium homeostasis, by activating Ca2+ release from internal stores and regulating Ca2+ fluxes across the plasma membrane, ii) plasma membrane potential variat…
Serratula tinctoria (L.) (Dyer's savory): In vitro culture and the production of ecdysteroids and other secondary metabolites
1996
The name Serratula is derived from the Latin word serra, which means saw. It designates perennial plants of the Compositae family with medium-sized, serrated leaves and purple flowers (Loste 1937). More than 40 species have been described in Europe, North Africa, and Asia. The most common is Serratula tinctoria, also known as dyer’s savory. This species, 30 to 80 cm high, grows on all kind of soils, and is widely distributed in central and northern Europe (synonym: Carduus tinctoria, Scop; French name: serratule des teinturiers; German name: Faberscharte). In France, for example, Serratula tinctoria is widespread but with an irregular distribution, rare in the north and near the Mediterrane…
Agro-ecological benefits of faba bean for rainfed Mediterranean cropping systems
2017
This paper reviews the main results from a set of experiments carried out in a semiarid Mediterranean environment during the past 25 years on faba bean (<em>Vicia faba</em> L.), a crop traditionally grown in southern Italy and Sicily under rainfed conditions. These experiments focused on the residual effects of faba bean on subsequent crop(s) and assessment of the nitrogen (N) balance during the crop cycle, paying attention to both the environmental release of N (losses via volatilisation and denitrification) and estimates of N2 fixation as influenced by tillage system, intercropping, and presence/absence of mycorrhizal inoculum. Faba bean relied on N2 fixation more than other g…
Contribution to the knowledge of Mediterranean wetland vegetation: Lemnetea and Potamogetonetea classes in Western Sicily
2021
The freshwater aquatic vegetation of the Lemnetea and Potamogetonetea classes in Western Sicily was surveyed and analyzed. 85 lakes and small pools were investigated collecting 147 phytosociological unpublished relevés to integrate the very scarce available data (only 3 relevés). By applying statistical analyses on abundances data and on the bases of physiognomy and dominant species, two alliances (Lemnion minoris and Stratiotion) and four different coenoses have been identify for the Lemnetea class; while 11 associations, assigned to two orders (Potamogetonetalia pectinati and Callitricho hamulatae-Ranunculetalia aquatilis) and four alliances (Potamogetonion, Nymphaeion albae, Ranunculion …
Fire and Plant Diversification in Mediterranean-Climate Regions
2018
Despite decades of broad interest in global patterns of biodiversity, little attention has been given to understanding the remarkable levels of plant diversity present in the world’s five Mediterranean-type climate (MTC) regions, all of which are considered to be biodiversity hotspots. Comprising the Mediterranean Basin, California, central Chile, the Cape Region of South Africa, and southwestern Australia, these regions share the unusual climatic regime of mild wet winters and warm dry summers. Despite their small extent, covering only about 2.2% of world land area, these regions are home to approximately one-sixth of the world vascular plant flora. The onset of MTCs in the middle Miocene …
Iodine Biofortification Counters Micronutrient Deficiency and Improve Functional Quality of Open Field Grown Curly Endive
2021
Human iodine (I) shortage disorders are documented as an imperative world-wide health issue for a great number of people. The World Health Organization (WHO) recommends I consumption through ingestion of seafood and biofortified food such as vegetables. The current work was carried out to appraise the effects of different I concentrations (0, 50, 250, and 500 mg L−1), supplied via foliar spray on curly endive grown in the fall or spring–summer season. Head fresh weight, stem diameter, head height, and soluble solid content (SSC) were negatively correlated to I dosage. The highest head dry matter content was recorded in plants supplied with 250 mg I L−1, both in the fall and spring–summer se…
Evidence for a geranyl-diphosphate synthase located within the plastids of Vitis vinifera L. cultivated in vitro
1992
Intact plastids from cell suspensions of Vitis vinifera L. cv. Muscat de Frontignan, free of detectable contamination by other particles as judged by the distribution of organelle-specific marker enzymes and by electron microscopy, exhibit geranyl-diphosphate synthase activity (EC 2.5.1.1). This synthase activity remains stable after tryptic digestion of unlysed organelles and is enhanced by plastid disruption. We conclude that the enzyme is located within the organelle. The possibility of an isopentenyl diphosphate/dimethylallyl diphosphate translocating system which would play a major role in the regulation of monoterpene metabolism is discussed.
Influence of Preharvest Gibberellic Acid Treatments on Postharvest Quality of Minimally Processed Leaf Lettuce and Rocket
2019
Plant growth regulators are used in high-value vegetable crops during cultivation and after harvest to increase yield, enhance crop management, and improve or retain the produce quality. The aim of this work was to evaluate the quality characteristics during cold storage of minimally processed leaf lettuce and rocket, obtained from plants grown in a hydroponic floating system with mineral nutrient solutions (MNS) containing different levels of gibberellic acid (GA3). Plants were grown in greenhouse conditions on nutrient solutions containing 0, 10&minus
Regulation of Nicotiana tabacum osmotic stress-activated protein kinase and its cellular partner GAPDH by nitric oxide in response to salinity
2010
Several studies focusing on elucidating the mechanism of NO (nitric oxide) signalling in plant cells have highlighted that its biological effects are partly mediated by protein kinases. The identity of these kinases and details of how NO modulates their activities, however, remain poorly investigated. In the present study, we have attempted to clarify the mechanisms underlying NO action in the regulation of NtOSAK (Nicotiana tabacum osmotic stress-activated protein kinase), a member of the SNF1 (sucrose non-fermenting 1)-related protein kinase 2 family. We found that in tobacco BY-2 (bright-yellow 2) cells exposed to salt stress, NtOSAK is rapidly activated, partly through a NO-dependent pr…
Bacterial rhamnolipids are novel MAMPs conferring resistance to Botrytis cinerea in grapevine
2009
International audience; Rhamnolipids produced by the bacteria Pseudomonas aeruginosa are known as very efficient biosurfactant molecules. They are used for a wide range of industrial applications, especially in food, cosmetics and pharmaceutical formulations as well as in bioremediation of pollutants. In this paper, the role of rhamnolipids as novel molecules triggering defence responses and protection against the fungus Botrytis cinerea in grapevine is presented. The effect of rhamnolipids was assessed in grapevine using cell suspension cultures and vitro-plantlets. Ca2+ influx, mitogen-activated protein kinase activation and reactive oxygen species production form part of early signalling…